2007
DOI: 10.1016/j.gene.2007.02.044
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A neuroglobin-overexpressing transgenic mouse

Abstract: Neuroglobin (Ngb) is a recently discovered vertebrate globin expressed primarily in neurons. Ngb expression is induced by hypoxia and ischemia, and Ngb protects neurons from these insults. However, its normal physiological role and the mechanism underlying its neuroprotective action are uncertain. We report production of a transgenic mouse in which Ngb is overexpressed under the control of the chicken β-actin promoter. This mouse should prove helpful for studying Ngb-mediated effects in vitro and in vivo.

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Cited by 29 publications
(38 citation statements)
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“…All previous experimental stroke reports of neuroprotection afforded by Ngb have been determined in normal mice or rats or using transgenic overexpression. [8][9][10][11] In using SHRSP, which exhibit a number of stroke-related comorbidities, we have addressed STAIR guidelines 5 as hypertension was reported as the strongest risk factor for stroke in a global case study. 7 Furthermore, all analyses were performed under masked conditions with animals randomly assigned to experimental groups and blood pressure monitoring throughout, in accordance with the preclinical stroke study guidelines.…”
Section: Discussionmentioning
confidence: 99%
“…All previous experimental stroke reports of neuroprotection afforded by Ngb have been determined in normal mice or rats or using transgenic overexpression. [8][9][10][11] In using SHRSP, which exhibit a number of stroke-related comorbidities, we have addressed STAIR guidelines 5 as hypertension was reported as the strongest risk factor for stroke in a global case study. 7 Furthermore, all analyses were performed under masked conditions with animals randomly assigned to experimental groups and blood pressure monitoring throughout, in accordance with the preclinical stroke study guidelines.…”
Section: Discussionmentioning
confidence: 99%
“…Ngb-Tg mice were generated as described previously (2,3). Ngb ϩϩ /APP Sw,Ind transgenic mice were produced by crossing homozygous Ngb ϩϩ transgenic mice (FVB ϫ CD1) with heterozygous APP Sw,Ind line J9 transgenic mice (C57BL/6 ϫ DBA/2), which express human APP with Swedish (K670N and M671L) and Indiana (V717F) AD mutations and overproduce human A␤.…”
Section: Methodsmentioning
confidence: 99%
“…Moreover, raft polarization precedes caspase activation and mitochondrial release of cytochrome c, indicating that it is an earlyand, thus, potentially reversible-event in cell-death pathways. Neurons from Ngb-overexpressing transgenic (Ngb-Tg) mice (2,3) do not undergo membrane polarization in response to hypoxia and are resistant to hypoxic cell death, suggesting that Ngb may protect neurons from hypoxia by interfering with this cell-death signaling complex.…”
mentioning
confidence: 99%
“…Thus, enhancing Ngb expression reduces-and knocking down Ngb expression increases-hypoxic neuronal injury in vitro [21] and ischemic cerebral injury in vivo [22]. In addition, Ngb-overexpressing transgenic mice are resistant to cerebral infarction from occlusion of the middle cerebral artery [10,11]. Ngb also reduces the toxicity of H 2 O 2 [5], paraquat [5] and β-amyloid [13] in vitro.…”
Section: Introductionmentioning
confidence: 99%