A New 42-Kda Protein-Binding to the Growth Suppressor Protein-P53

Appel, K; Schneider, Eberhard; Wagner, P.; Jo, Höög; Montenarh, Mathias; Karlsson, Christina

doi:10.3892/ijo.5.3.667

Cited by 3 publications

(1 citation statement)

References 0 publications

Supporting

Mentioning

Contrasting

Order By: Relevance

“…In addition this tmsl-related 42 kDa protein was localized to the nucleus of various mammalian cell lines [28]. More recently we were able to establish the p53 binding site on the tmsl protein to the sequence YITTEDFCT (aa 1 lf~125) in the vicinity of a well conserved cell division motif [29].…”

Section: Introductionmentioning

confidence: 93%

Precise mapping of the tms1 binding site on p53

et al. 1995

View full text Add to dashboard Cite

Originally identified as multicopy suppressor of a lethal growth arrest caused by expression of a tumour mutant cDNA of p53 in fission yeast the tmsl gene product was found to form stable complexes with p53 in yeast. By using purified recombinant proteins multimeric complexes of tmsl and p53 could be demonstrated and recently the p53 binding site on the tmsl protein was established to the sequence YYITTEDFCT (aa 116-125) in the vicinity of a well conserved cell division motif. Here we report the precise mapping of the tmsl binding site on the p53 protein to the sequence LQIRGRERFE (aa 330-339) which defines a new functional domain on the p53 protein.

show abstract

Section: Introductionmentioning

confidence: 93%

Precise mapping of the tms1 binding site on p53

et al. 1995

View full text Add to dashboard Cite

show abstract

Protein kinase CK2 interacts with a multi-protein binding domain of p53

Götz

Scholtes

Prowald

et al. 1999

A Molecular and Cellular View of Protein Kinase CK2

View full text Add to dashboard Cite

Fission yeast tmsl protein abrogates normal development in Xenopus laevis embryos

Wagner

Hoever

Appel

et al. 1995

Roux's Arch Dev Biol

View full text Add to dashboard Cite

Recently we cloned tms1 (a putative dehydrogenase) by complementation of a human tumour-derived mutant p53 induced growth arrest in fission yeast. Microinjection of purified tmsl protein into Xenopus laevis embryos abrogated normal embryo development by causing cleavage retardation or cleavage arrest of injected blastomeres in a concentration dependant manner, whereas injection of specific affinity purified tms1 antiserum showed no significant morphological defects. Microinjection of tms1 protein together with affinity purified tms1 antibody resulted in a significantly reduced number of cleavage arrested embryos.

show abstract

A New 42-Kda Protein-Binding to the Growth Suppressor Protein-P53

Cited by 3 publications

References 0 publications

Precise mapping of the tms1 binding site on p53

Precise mapping of the tms1 binding site on p53

Protein kinase CK2 interacts with a multi-protein binding domain of p53

Fission yeast tmsl protein abrogates normal development in Xenopus laevis embryos

Contact Info

Product

Resources

About