A New Actinomycete Species, Nocardiopsis lucentensis sp. nov.

Yassin, A. F.; Galinski, Erwin A.; Wohlfarth, Axel; Jahnke, K.-D.; Schaal, K. P.; Trüper, Hans G.

doi:10.1099/00207713-43-2-266

Cited by 77 publications

(62 citation statements)

References 32 publications

Supporting

Mentioning

Contrasting

Order By: Relevance

“…Grund and Kroppenstedt (10) found that Nocardiopsis dassonvillei and the two subspecies of Nocardiopsis alba have the same menaquinone composition. A different menaquinone composition was found for Nocardiopsis alborubida and Nocardiopsis listen (lo), while a third type was reported for Nocardiopsis lucentensis (27). This apparent lack of congruence provides another example of the difficulties involved in comparing the evolution of menaquinones (9).…”

Section: Taccgcggc[t/g]gctg3') 536f (5'cagc[c/a]gccgcggtaat[t/a]cmentioning

confidence: 99%

“…To date, a comprehensive DNA-DNA hybridization study of all Nocardiopsis species has not been carried out. However, the limited DNA-DNA hybridization data presented in the description of Nocardiopsis lucentensis (27) could be compared with the 16s rDNA sequence analysis data. These DNA-DNA hybridization data demonstrated that Nocardiopsis lucentensis is a true species when this organism was compared with Nocardiopsis dassonvillei, Nocardiopsis alborubida, Nocardiopsis listen', Nocardiopsis alba subsp.…”

Section: Taccgcggc[t/g]gctg3') 536f (5'cagc[c/a]gccgcggtaat[t/a]cmentioning

confidence: 99%

See 1 more Smart Citation

The Genus Nocardiopsis Represents a Phylogenetically Coherent Taxon and a Distinct Actinomycete Lineage: Proposal of Nocardiopsaceae fam. nov.

Rainey

Ward-Rainey²,

Kroppenstedt³

et al. 1996

International Journal of Systematic Bacteriology

942

630

View full text Add to dashboard Cite

The genus Nocardiopsis was shown to be phylogenetically coherent and to represent a distinct lineage within the radiation of the order Actinomycetales. The closest relatives of the genus Nocardiopsis are members of the genera Actinomadura, Thermomonospora, Streptosporangium, and Microtetraspora. The intrageneric structure of the genus Nucardiupsis is shown to consist of a highly related species group containing Nucardiupsis dassonvillei, Nocardiopsis alborubida, and Nocardiopsis antarctica and a second group of less highly related species comprising Nocardiopsis alba subs p. alba, Nocardiopsis alba subs p. prasina, and Nocardiopsis listeri. Nocardiopsis lucentensis occupies a position intermediate between the two species groups. The results of a 16s ribosomal DNA sequence analysis are generally consistent with the available chemotaxonomic, phenotypic, and DNA-DNA hybridization data. The phylogenetic position and the morpho-and chemotaxonomic properties of Nocardiopsis species support the creation of a family for the genus Nocardiopsis, Nocardiopsaceae fam. nov.

show abstract

Section: Taccgcggc[t/g]gctg3') 536f (5'cagc[c/a]gccgcggtaat[t/a]cmentioning

confidence: 99%

Section: Taccgcggc[t/g]gctg3') 536f (5'cagc[c/a]gccgcggtaat[t/a]cmentioning

confidence: 99%

The Genus Nocardiopsis Represents a Phylogenetically Coherent Taxon and a Distinct Actinomycete Lineage: Proposal of Nocardiopsaceae fam. nov.

Rainey

Ward-Rainey²,

Kroppenstedt³

et al. 1996

International Journal of Systematic Bacteriology

942

630

View full text Add to dashboard Cite

show abstract

“…At the time of writing, the taxon encompasses 28 recognized species and 4 subspecies, which form a distinct clade within the evolutionary radiation occupied by members of the family Nocardiopsaceae (Rainey et al, 1996). Nocardiopsis strains are frequently isolated from saline soils (Yassin et al, 1993a;Li et al, , 2006, but they have also been recovered from an alkaline slag dump (Schippers et al, 2002), indoor environments (Peltola et al, 2001), the atmosphere of a composting facility (Kämpfer et al, 2002) and clinical material (Bernatchez & Lebreux, 1991;Yassin et al, 1997). In the present polyphasic study, an actinomycete isolated from household waste was shown to represent a novel species of the genus Nocardiopsis.…”

mentioning

confidence: 99%

Nocardiopsis potens sp. nov., isolated from household waste

Yassin

Spröer

Hupfer

et al. 2009

INTERNATIONAL JOURNAL OF SYSTEMATIC AND EVOLUTIONARY MICROBIOLO

Self Cite

View full text Add to dashboard Cite

The taxonomic position of an actinomycete, designated strain IMMIB L-21 T , was determined using a polyphasic taxonomic approach. The organism, which had phenotypic properties consistent with its classification in the genus Nocardiopsis, formed a distinct clade in the 16S rRNA gene sequence tree together with the type strain of Nocardiopsis composta, but was readily distinguished from this species using DNA-DNA relatedness and phenotypic data. The genotypic and phenotypic data show that the organism represents a novel species of the genus Nocardiopsis, for which the name Nocardiopsis potens sp. nov. is proposed. The type strain is IMMIB L-21 T (5DSM 45234 T 5CCUG 56587 T ).The genus Nocardiopsis was proposed by Meyer (1976) on the basis of chemotaxonomic and morphological characteristics. At the time of writing, the taxon encompasses 28 recognized species and 4 subspecies, which form a distinct clade within the evolutionary radiation occupied by members of the family Nocardiopsaceae (Rainey et al., 1996). Nocardiopsis strains are frequently isolated from saline soils (Yassin et al., 1993a;Li et al., , 2006, but they have also been recovered from an alkaline slag dump (Schippers et al., 2002), indoor environments (Peltola et al., 2001), the atmosphere of a composting facility (Kämpfer et al., 2002) and clinical material (Bernatchez & Lebreux, 1991;Yassin et al., 1997). In the present polyphasic study, an actinomycete isolated from household waste was shown to represent a novel species of the genus Nocardiopsis.Strain IMMIB L-21 T was isolated from a household dustbin on Brain Heart Infusion (BHI; BD) agar, glucose-yeast extract-malt extract agar (GYM; medium 65; DSMZ) and Columbia agar (BD) supplemented with 5 % sheep blood. The organism was grown on yeast extract-malt extract agar (ISP 2), oatmeal agar (ISP 3) and inorganic salts-starch agar (ISP 4) as described by Shirling & Gottlieb (1966) and examined for pigmentation and colour of aerial and substrate mycelia. Gram and ZiehlNeelsen stains were applied to hyphae grown on Columbia agar. Growth at different temperatures was determined at 10, 20, 27, 37 and 42 u C and a range of phenotypic characteristics was examined using standard procedures (Gordon, 1966(Gordon, , 1967Gordon & Mihm, 1957;Yassin et al., 1995). Tolerance to NaCl (0-16 %) was tested in GYM broth. Biomass for chemotaxonomic studies was derived from a 7-day-old BHI broth shake culture incubated at 37 u C, harvested by centrifugation and washed with distilled water. Standard procedures were used to determine the isomeric form of diaminopimelic acid (Becker et al., 1964), whole-cell sugars (Lechevalier, 1968), nonhydroxylated fatty acids (Minnikin et al., 1980;Yassin et al., 2007), polar lipids (Yassin et al., 1993b) and isoprenoid quinones (Collins et al., 1977;Yassin & Hupfer, 2006). Genomic DNA extraction and PCR-mediated amplification of the 16S rRNA gene sequence were carried out using established procedures (Rainey et al., 1996). The purified PCR products were sequenced using a Taq DyeDeoxy...

show abstract

“…alba, Nocardiopsis alba subsp. prasina and Nocardiopsis synnemataformans (Meyer, 1976 ;Grund & Kroppenstedt, 1990 ;Abyzov et al, 1983 ;Yassin et al, 1993Yassin et al, , 1997Al-Tai & Ruan, 1994 ;Miyashita et al, 1984). The subspecies N. alba subsp.…”

Section: Introductionmentioning

confidence: 99%