A new agglutinating activity from wheat flour inhibited by tryptophan

A comparative study of the localization of wheat-germ agglutinin and its potential receptors in wheat grains

“…Since all haemagglutination activity measured could be inhibited by N-acetyl-D-glucosamine, it is highly probable that wheat-germ agglutinin is the lectin involved. This is further substantiated by evidence (results not shown) that the Pronase-treated erythrocytes used in this assay could not be agglutinated by the polysaccharide agglutinin of endosperm tissues (Minetti et al, 1976).…”

Naturally occurring polysaccharide derivatives which behave as an artificial cell wall on an artificial cell liposome

“…8 In order to mimic the morphology and function of the cell walls of plant and bacterial cell membranes, several water-soluble and naturally occurring polysaccharides have been hydrophobized by fatty acids. For example, Tsumita and Ohashi first demonstrated that a polysaccharide hydrophobized with acyl groups easily adsorbs onto the erythrocyte surface.11 Thereafter, it has been reported that such acylated polysaccharides adhere to the surfaces of cytoplasm membranes,12•13 planar lipid bilayer membranes,14 and liposomes.16"17 Sunamoto and his co-workers have substituted the palmitoyl group as a hydrophobic anchor to several naturally occurring polysaccharides.16 •18 They also confirmed the improved stability of the liposome upon coating with O-palmitoyl-substituted polysaccharide from two aspects: (1) a decrease in the permeability to a water-soluble probe, 6-carboxyfluorescein (CF), which is encapsulated in the interior water pool of the liposome, and (2) an increase in the resistance of the liposome against enzymatic lyses with phospholipase D and lipid peroxidase. 16•18 Furthermore, they have first employed such polysaccharide-coated liposomes as a receptor-mediated drug carrier.18 •19 Other water-soluble polymers such as polypeptides20 also have been utilized to coat the surface of vesicles besides • To whom correspondence should be addressed.…”

Interaction of neutral polysaccharides with phosphatidylcholine multilamellar liposomes. Phase transitions studied by the binding of fluorescein-conjugated dextrans

“…One possible approach to an investigation into the arrangement of polysaccharides on the cell surface and polysaccharide involvement in biological processes is the study of the interaction between membranes and chemically defined polysaccharides. It is known that neutral polysaccharides can interact with cell membranes and induce cell aggregation (Brooks & Seaman, 1972;Brooks, 1973; Jan & Chien, 1973;Minetti et al, 1976). Cell surface interaction with neutral polymer has been used to separate cell populations (Uhlenbruck et al, 1967;Walter et al, 1972).…”