A new alkalophilic isolate of Bacillus as a producer of cyclodextrin glycosyltransferase using cassava flour

Coelho, Sheila Lorena de Araújo; Magalhães, Valter Cruz; Marbach, Phellippe Arthur Santos; Cazetta, Márcia Luciana

doi:10.1016/j.bjm.2015.11.018

Cited by 9 publications

(6 citation statements)

References 55 publications

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“…The formation of the fisetin/cyclodextrin inclusion complex was also reported in order to improve the property of fisetin ( Zhang et al, 2015 ). CGTase can be found in various bacterial strains such as Bacillus ( Arce-Vázquez et al, 2016 ; de Araújo Coelho et al, 2016 ; Tao et al, 2019 ; van der Veen et al, 2000 ), Pseudomonas ( Jiang et al, 2018 ), and Thermoanaerobacterium ( Leemhuis, Dijkstra & Dijkhuizen, 2003 ). CGTase was used to synthesize various flavonoid glycosides using various flavonoid acceptors such as hesperidin ( Kometani et al, 1994 ), hesperetin ( González-Alfonso et al, 2018 ), naringin ( Kometani et al, 1996 ), epicatechin ( Aramsangtienchai et al, 2011 ) and resveratrol ( Torres et al, 2011 ).…”

Section: Introductionmentioning

confidence: 99%

Fisetin glycosides synthesized by cyclodextrin glycosyltransferase from Paenibacillus sp. RB01: characterization, molecular docking, and antioxidant activity

Lorthongpanich

Mahalapbutr

Rungrotmongkol

et al. 2022

PeerJ

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Fisetin is a flavonoid that exhibits high antioxidant activity and is widely employed in the pharmacological industries. However, the application of fisetin is limited due to its low water solubility. In this study, glycoside derivatives of fisetin were synthesized by an enzymatic reaction using cyclodextrin glycosyltransferase (CGTase) from Paenibacillus sp. RB01 in order to improve the water solubility of fisetin. Under optimal conditions, CGTase was able to convert more than 400 mg/L of fisetin to its glycoside derivatives, which is significantly higher than the previous biosynthesis using engineered E. coli. Product characterization by HPLC and LC-MS/MS revealed that the transglycosylated products consisted of at least five fisetin glycoside derivatives, including fisetin mono-, di- and triglucosides, as well as their isomers. Enzymatic analysis by glucoamylase and α-glucosidase showed that these fisetin glycosides were formed by α-1,4-glycosidic linkages. Molecular docking demonstrated that there are two possible binding modes of fisetin in the enzyme active site containing CGTase-glysosyl intermediate, in which O7 and O4’ atoms of fisetin positioned close to the C1 of glycoside donor, corresponding to the isomers of the obtained fisetin monoglucosides. In addition, the water solubility and the antioxidant activity of the fisetin monoglucosides were tested. It was found that their water solubility was increased at least 800 times when compared to that of their parent molecule while still maintaining the antioxidant activity. This study revealed the potential application of CGTase to improve the solubility of flavonoids.

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Section: Introductionmentioning

confidence: 99%

Fisetin glycosides synthesized by cyclodextrin glycosyltransferase from Paenibacillus sp. RB01: characterization, molecular docking, and antioxidant activity

Lorthongpanich

Mahalapbutr

Rungrotmongkol

et al. 2022

PeerJ

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“…According to quantitative screening, 5 potent CGTase-producing strains were selected and identified as B. [51,54,55]. Nowadays, RSM is widely reported as an effective statistical technique for designing experiments, building models, evaluating the interactive effects of variables, and pointing the optimum conditions to improve the complex biological processes, especially enzyme production [56−59].…”

Section: Discussionmentioning

confidence: 99%

Enhancement of ��-Cyclodextrin Production and Fabrication of Edible Antimicrobial Films Incorporated with Clove Essential Oil/��-cyclodextrin Inclusion Complex

Farahat¹

2020

Microbiology and Biotechnology Letters

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Edible films containing antimicrobial agents can be used as safe alternatives to preserve food products. Essential oils are well-recognized antimicrobials. However, their low water solubility, volatility and high sensitivity to oxygen and light limit their application in food preservation. These limitations could be overcome by embedding these essential oils in complexed product matrices exploiting the encapsulation efficiency of β-cyclodextrin. This study focused on the maximization of β-cyclodextrin production using cyclodextrin glucanotransferase (CGTase) and the evaluation of its encapsulation efficacy to fabricate edible antimicrobial films. Response surface methodology (RSM) was used to optimize CGTase production by Brevibacillus brevis AMI-2 isolated from mangrove sediments. This enzyme was partially purified using a starch adsorption method and entrapped in calcium alginate. Cyclodextrin produced by the immobilized enzyme was then confirmed using high performance thin layer chromatography, and its encapsulation efficiency was investigated. The clove oil/β-cyclodextrin inclusion complexes were prepared using the coprecipitation method, and incorporated into chitosan films, and subjected to antimicrobial testing. Results revealed that β-cyclodextrin was produced as a major product of the enzymatic reaction. In addition, the incorporation of clove oil/β-cyclodextrin inclusion complexes significantly increased the antimicrobial activity of chitosan films against Staphylococcus aureus, Staphylococcus epidermidis, Salmonella Typhimurium, Escherichia coli, and Candida albicans. In conclusion, B. brevis AMI-2 is a promising source for CGTase to synthesize β-cyclodextrin with considerable encapsulation efficiency. Further, the obtained results suggest that chitosan films containing clove oils encapsulated in β-cyclodextrin could serve as edible antimicrobial food-packaging materials to combat microbial contamination.

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“…b β-CGTase-H167C was produced with different fermentation temperature with initial pH value at 6.0. c Bacterial strain expressing β-CGTase-H167C was seeded with the indicated inoculum size and the activity of β-CGTase-H167C was detected at each condition. d Different shaking speed was adapted and the production of β-CGTase-H167C was examined Bacillus represented optimum activity at pH 5.0 (Coelho et al 2016). These results indicated that the optimal pH values of CGTase produced by different microbial strains are variable.…”

Section: Initial Phmentioning

confidence: 99%

“…Conventionally, the production of CGTase was achieved by incubating the bacteria with the culture medium that contains essential components for the growth of the microbial. Among the elements in the medium, the carbon and nitrogen sources were obtained with high cost and complex technical issues (Coelho et al 2016). To solve this problem, agro-industrial origins of carbon and nitrogen sources have drawn wide attention (Akindahunsi and Oboh 2003;Brauman et al 1996;Lepiz-Aguilar et al 2013).…”

Section: Introductionmentioning

confidence: 99%

“…Cassava flour, an agro-industrial product rich in starch, can be simply obtained and has been an alternative candidate for the carbon source 1 3 (Akindahunsi and Oboh 2003;Brauman et al 1996;Lepiz-Aguilar et al 2013). Additionally, it has been reported that the corn steep liquor originated from corn processing and is commonly used as the nitrogen source in fermentation (Coelho et al 2016;Ebadipour et al 2016;He et al 2017;Wischral et al 2016). To overall improve the growth of microbial and the synthesis of enzyme, other essential elements in the culture medium including metal ions and phosphate also need to the optimized.…”

Section: Introductionmentioning

confidence: 99%

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Optimization of the fermentation conditions for the mutant strain of β-cyclodextrin glycosyltransferase H167C to produce cyclodextrins

Wang

Zhou

et al. 2018

3 Biotech

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The cyclodextrin glycosyltransferase (CGTase) was used to catalyze the conversion of starch into cyclodextrins (CD) in industry. Improving the activity of CGTase to produce more CD with relative low cost is intensely interesting and has drawn wide attention. Amino acid mutation of His167 into Cys significantly enhanced β-CGTase activity; however, optimization of culture conditions for β-CGTase-H167C remains unclear. To determine this, the medium and culture conditions for β-CGTase-H167C were optimized with response surface methodology. Maximum activity of β-CGTase-H167C was obtained with the medium containing 1.1% corn starch, 4.4% corn steep liquor, 1.1% peptone, 0.02% MgSO·7HO and 0.1% KHPO·3HO that were cultured with the initial pH 8.4, incubation temperature at 37.4 °C, with 5% inoculation size and shaking speed at 202 r/min. Under the optimal conditions, the activity of β-CGTase-H167C was up to 4355 U/mL, which is 1.93-fold in comparison with the initial activity. Our results established the promising culture strategy for the production of cyclodextrins by β-CGTase-H167C.

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A new alkalophilic isolate of Bacillus as a producer of cyclodextrin glycosyltransferase using cassava flour

Cited by 9 publications

References 55 publications

Fisetin glycosides synthesized by cyclodextrin glycosyltransferase from Paenibacillus sp. RB01: characterization, molecular docking, and antioxidant activity

Fisetin glycosides synthesized by cyclodextrin glycosyltransferase from Paenibacillus sp. RB01: characterization, molecular docking, and antioxidant activity

Enhancement of ��-Cyclodextrin Production and Fabrication of Edible Antimicrobial Films Incorporated with Clove Essential Oil/��-cyclodextrin Inclusion Complex

Optimization of the fermentation conditions for the mutant strain of β-cyclodextrin glycosyltransferase H167C to produce cyclodextrins

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