Suitable conditions for passive sampler collection of NO isotopes are in environments ranging from 11 to 34 °C and 1 to 78% RH. The passive NO isotope measurement technique provides an accurate method to determine variations in atmospheric δ N-NO values and a precise method for determining atmospheric δ O-NO values. The ability to measure NO isotopes over spatial gradients at the same temporal resolution provides a unique perspective on the extent and seasonality of fluctuations in atmospheric NO isotopic compositions. Copyright © 2017 John Wiley & Sons, Ltd.