A new amperometric cholesterol biosensor based on poly(3,4-ethylenedioxypyrrole)

Türkarslan, Özlem; Kayahan, Senem Kiralp; Toppare, Levent

doi:10.1016/j.snb.2008.10.016

Cited by 65 publications

(41 citation statements)

References 31 publications

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“…Çalışma sonucunda 0,03 Ünite µL -1 XO optimum enzim miktarı olarak seçildi ve karşılaştırma açısından diğer tip MWCNT'ler ile hazırlanan elektrotlarda da aynı miktar enzim kullanıldı. Enzimlerin aktiviteleri pH ile değiştiğinden tampon pH'sının biyosensör cevabı üzerinde önemli bir etkisi vardır [29]. Bu etkiyi araştırmak amacı ile NH2-MWCNT ile modifiye edilmiş enzim elektrot örnek matriks olarak kullanıldı ve 6,0-8,5 aralığında 0,5 pH birimi değişen pH'larda biyosensörün 0,02 mM ksantine cevabı belirlendi.…”

Section: Elektrot Bileşiminin Optimizasyonuunclassified

Ksantin Biyosensörlerin Performansına Çeşitli Çok Duvarlı Karbon Nanotüplerin Etkisi

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Erden

et al. 2018

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Özet: Bu çalışmada, fonksiyonel grup içermeyen (MWCNT) ve karboksil (COOH-MWCNT), hidroksil (OH-MWCNT) ve amino (NH2-MWCNT) grubu içeren çok duvarlı karbon nanotüplerin amperometrik ksantin biyosensörünün cevabına etkileri incelendi. Bu amaçla, MWCNT'ler ve ksantin oksidaz (XO) enzimi jelatin çözeltisi içinde dağıtıldı ve bu karışımlardan belirli miktarların camsı karbon elektrot yüzeyine damlatılması ile ksantin biyosensörleri hazırlandı. Bu biyosensörlerin optimum pH, enzim miktarı gibi çalışma koşulları belirlendi ve bu koşullarda biyosensörlerin performans faktörleri incelendi. NH2-MWCNT ile hazırlanan biyosensörün en yüksek duyarlık ile ksantine cevap verdiği gözlendi. Bu biyosensör ksantine <15 s gibi kısa bir cevap süresi ile 9,9×10 -7 M -9,4×10 -4 M aralığında doğrusal cevap gösterdi ve gözlenebilme sınırı 8,7×10 -7 M, duyarlığı 23,36 µA mM -1 olarak belirlendi. The Effect of Various Multiwalled Carbon Nanotubes on the Performance of Xanthine BiosensorsKeywords Biosensor, Amperometry, Multiwalled carbon nanotube, Xanthine, Xanthine oxidase Abstract:In this study effects of multiwalled carbon nanotubes (MWCNT), and multiwalled carbon nanotubes with carboxyl (COOH-MWCNT), hydroxyl (OH-MWCNT) and amino (NH2-MWCNT) functional groups on the response of amperometric xanthine biosensors were investigated. For this purpose, MWCNT and xanthine oxidase (XO) enzyme were dispersed in gelatin solution and aliquots of this solution was drop casted onto the surface of glassy carbon electrode in order to prepare the xanthine biosensors. Working conditions such as optimum pH and enzyme amount of these biosensors were investigated and analytical performances of the biosensors were determined under this optimum conditions. The biosensor prepared with NH2-MWCNT exhibited a xanthine response with the highest sensitivity. The response time, linear working range, detection limit and sensitivity of this biosensor was <15 s; 9.9×10 -7 M -9.4×10 -4 M; 8.7×10 -7 M; 23.36 µA mM -1 respectively.

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Section: Elektrot Bileşiminin Optimizasyonuunclassified

Ksantin Biyosensörlerin Performansına Çeşitli Çok Duvarlı Karbon Nanotüplerin Etkisi

Kaçar

Dalkıran

Erden

et al. 2018

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“…Although different temperatures were reported as optimum, it was observed that in cholesterol biosensors based on polymer films were carried out at room temperature. 21,23 For practical applications, following experiments were carried out at room temperature (23 ± 2 °C).…”

Section: Effects Of Buffer Concentration and Phmentioning

confidence: 99%

“…13,17,21,23,36,59 In many studies, cholesterol contents of synthetic serum samples like Accutrol™ were determined. 16, 35 Brahim et al 14 reported a amperometric biosensor to the determination of total cholesterol in serum samples with a correlation coefficient of 0.998.…”

Section: Analysis Of the Total Cholesterol In Blood Serummentioning

confidence: 99%

Development of an amperometric enzyme electrode based on poly(o-phenylenediamine) for the determination of total cholesterol in serum

Zeybek¹,

Zeybek²,

Pekmez³

et al. 2012

J. Braz. Chem. Soc.

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Um eletrodo enzimático amperométrico baseado em polímero foi preparado para detecção amperométrica de colesterol livre. Inicialmente, o filme polimérico poli(o-fenilenodiamina) (PoPD) foi eletropolimerizado no meio acetonitrila-água contendo o monômero o-fenilenodiamina (oPD) e ácido canforsulfônico em eletrodo de Pt através da técnica de voltametria cíclica. A enzima oxidase colesterol foi imobilizada sobre a superfície do eletrodo Pt/PoPD. A determinação de colesterol foi realizada por maio do monitoramento da corrente de oxidação de H 2 O 2 produzido enzimaticamente a +0,7 V vs. Ag/AgCl. A concentração da solução tampão, o valor de pH e a temperatura de trabalho otimizados foram 0,05 mol L −1 , 7,5 and 40 °C, respectivamente. O intervalo de trabalho do eletrodo de enzima para colesterol foi 9,8 × 10 −3 -11 µmol L −1 e tempo de resposta 150 s. Os efeitos de possíveis interferências presentes em amostras de soro em resposta ao eletrodo de enzima foram examinados. A determinação do colesterol total em amostras de soro foi realizada usando o eletrodo de enzima Pt/PoPD/ChOx proposto e os resultados foram em bom acordo com os obtidos pelo método espectrofotométrico.A polymer-based amperometric enzyme electrode (Pt/PoPD/ChOx) was prepared for the amperometric detection of free cholesterol. Firstly, poly(o-phenylenediamine) (PoPD) polymer film was prepared in acetonitrile-water medium containing o-phenylenediamine (oPD) monomer and (±)-10-camphorsulfonic acid (HCSA) on Pt electrode by the use of cyclic voltammetry technique. Cholesterol oxidase (ChOx) enzyme was immobilized onto Pt/PoPD electrode surface. The determination of cholesterol was performed via monitoring of the oxidation current of enzymatically produced H 2 O 2 at +0.7 V vs. Ag/AgCl. Optimum buffer concentration, pH and working temperature were found as 0.05 mol L −1 , 7.5 and 40 °C, respectively. The working range of enzyme electrode to cholesterol was 9.8 × 10 −3 -11 µmol L −1 and response time 150 s. The effects of possible interferences present in serum samples on response of enzyme electrode were examined. The determination of total cholesterol in serum samples was performed by using proposed Pt/PoPD/ChOx enzyme electrode and results were in good agreement with those obtained by spectrophotometric method.

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“…Cholesterol levels, including high density lipoprotein cholesterol 20 (HDL-C) are modifiable risk factors for cardiovascular disease (CVD), a condition which remains the number one global cause of death.…”

Section: Introductionmentioning

confidence: 99%

“…[12][13][14] Electrochemical techniques lend themselves well to the fabrication of low cost, point of care and disposable diagnostic devices. Thus, it would seem a common 20 sense approach to develop electrochemical biosensor methodologies that are capable of measuring HDL-C using a similar principle, with the measurement of H 2 O 2 performed electrochemically. 15,16 While a number of electrochemical biosensors for cholesterol have been developed, [17][18][19][20][20][21][22][23][24][25][26][27][28][29][30] to date, 25 there are just a few published examples of electrochemical biosensors for HDL-C. 31,32 Kinoshita et al, developed an amperometric sensor based on a homogeneous assay in which, a peroxidase-entrapped and ferrocene-embeded carbon paste electrode was used to measure the H 2 O 2 produced after enzymatic 30 reaction of HDL-C. PEG-modified enzymes in the presence of -cyclodextrin sulphate and MgCl 2 were employed to impart selectivity to the measurement of HDL-C.…”

Section: Introductionmentioning

confidence: 99%

A biosensor for the determination of high density lipoprotein cholesterol employing combined surfactant-derived selectivity and sensitivity enhancements

2014

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A biosensor for the determination of high density lipoprotein cholesterol employing combined surfactant-derived selectivity and sensitivity enhancements. Analytical Methods, 6. pp. 3975-3981. ISSN 1759-9660 Available from: http://eprints.uwe.ac.uk/23377We recommend you cite the published version. The publisher's URL is: http://dx.doi.org/10.1039/C3AY42262C Refereed: YesPublished online first 7 Mar 2014 Disclaimer UWE has obtained warranties from all depositors as to their title in the material deposited and as to their right to deposit such material. UWE makes no representation or warranties of commercial utility, title, or fitness for a particular purpose or any other warranty, express or implied in respect of any material deposited. UWE makes no representation that the use of the materials will not infringe any patent, copyright, trademark or other property or proprietary rights. UWE accepts no liability for any infringement of intellectual property rights in any material deposited but will remove such material from public view pending investigation in the event of an allegation of any such infringement. PLEASE SCROLL DOWN FOR TEXT.Journal Name High density lipoprotein cholesterol (HDL-C) is a modifiable risk factor in cardiovascular disease and devices suitable for its determination at the point of care are critical to the future management of hypercholesterolaemia. An electrochemical biosensor for measuring HDL-C was developed. The 10 biosensor was based on a homogeneous assay methodology for selective determination of HDL-C in combination with a printed electrochemical sensor for measuring the reduction of hydrogen peroxide at a silver paste electrode. The polyoxyethylene alkylene tribenzylphenyl ether surfactant (Emulgen B-66) was found to be capable of both the selective dissolution of HDL particles, as well as the enhanced electrocatalytic reduction of hydrogen peroxide. The resulting biosensor was shown to have a linear 15 response to HDL-C from 0.5 to 4 mM (r 2 =0.998) with an average r.s.d. of 7%. The biosensor was also used to analyse HDL-C in thirteen serum samples and had good agreement with a commercial spectrophotometric precipitation-based assay (r=0.7222; p < 0.058). IntroductionCholesterol levels, including high density lipoprotein cholesterol 20 (HDL-C) are modifiable risk factors for cardiovascular disease (CVD), a condition which remains the number one global cause of death. 1-3 Levels of HDL-C above 60 mg/dL (1.55 mM) are considered to have a positive protective role in heart disease, while low HDL-C levels (less than 40 mg/dL or about 1 mM) are 25 linked to an increase in heart attack risk. For this reason, the importance of measurement of HDL-C has been emphasized by the National Cholesterol Education Programme (NCEP) since the late 1980s. 4,5 The measurement of HDL-C is also important for two other purposes: Network (CRMLN) developed a simpler method based on a modified dextran sulphate procedure. 8 However, this technique, like the CDC method, also required large sample volumes and a...

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A new amperometric cholesterol biosensor based on poly(3,4-ethylenedioxypyrrole)

Cited by 65 publications

References 31 publications

Ksantin Biyosensörlerin Performansına Çeşitli Çok Duvarlı Karbon Nanotüplerin Etkisi

Ksantin Biyosensörlerin Performansına Çeşitli Çok Duvarlı Karbon Nanotüplerin Etkisi

Development of an amperometric enzyme electrode based on poly(o-phenylenediamine) for the determination of total cholesterol in serum

A biosensor for the determination of high density lipoprotein cholesterol employing combined surfactant-derived selectivity and sensitivity enhancements

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