A new amplified π-shape electrochemical aptasensor for ultrasensitive detection of aflatoxin B1

Abnous, Khalil; Danesh, Noor Mohammad; Alibolandi, Mona; Ramezani, Mohammad; Emrani, Ahmad Sarreshtehdar; Zolfaghari, Reza; Taghdisi, Seyed Mohammad

doi:10.1016/j.bios.2017.03.028

Cited by 114 publications

(63 citation statements)

References 34 publications

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“…Abnous et al [31] built an electrochemical biosensor for accurate detection of AFB 1 . AFB 1 is based on aptamer to form a π-shape complementary strand of aptamer (CSs) complex on the surface of electrode and exonuclease I (Exo I).…”

Section: Electrochemical Aptamermentioning

confidence: 99%

A New Approach for Detection of Aflatoxin B1

Wang¹

2020

Aflatoxin B1 Occurrence, Detection and Toxicological Effects

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Aflatoxin B 1 (AFB 1 ) is harmful to human health, mainly resulting from its toxic effects on the liver. AFB 1 can lead to liver cell necrosis, hemorrhage, fibrosis, cirrhosis, etc. Acute AFB 1 exposure at high levels can lead to hepatitis, whereas chronic exposure can result in liver cancer. In the past decades, a series of methods and techniques for detecting AFB 1 , including enzyme-linked immunosorbent assay (ELISA), high-performance liquid chromatography (HPLC), and thin-layer chromatography (TLC), have been developed. This study reviewed the detection methods of AFB 1 and the corresponding utilization and summarizes all methods for evaluating the toxification of AFB 1 .

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Section: Electrochemical Aptamermentioning

confidence: 99%

A New Approach for Detection of Aflatoxin B1

Wang¹

2020

Aflatoxin B1 Occurrence, Detection and Toxicological Effects

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“…Among different aptamer‐based techniques, electrochemical aptasensors have been commonly used for routine analysis because of their simple signal production, high sensitivity and easy fabrication .…”

Section: Introductionmentioning

confidence: 99%

A Novel Electrochemical Aptasensor for Carcinoembryonic Antigen Detection Based on Target‐induced Bridge Assembly

Taghdisi

Danesh²,

Ramezani

et al. 2018

Electroanalysis

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The present study describes a novel electrochemical aptasensor for detection of carcinoembryonic antigen (CEA), a key cancer biomarker. The sensing strategy relied on the CEA‐induced bridge assembly, as a physical barrier, on the surface of gold electrode, resulting in a significant increase of the sensor sensitivity. Under optimal conditions, the aptasensing platform showed a wide linear range (3 pg/mL to 40 ng/mL) and a low detection limit (0.9 pg/mL). Some possible interfering materials were also assessed and the results indicated that the designed aptasensor had good specificity toward CEA. The quantitation of CEA in the spiked human serum samples confirmed the reliability and applicability of the electrochemical aptasensor. So, the developed sensing method has a potential application in the clinical diagnosis.

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“…Currently, high‐performance liquid chromatography (HPLC) with fluorescence detection, HPLC‐tandem mass spectrometry (HPLC‐MS/MS) , gas chromatography‐MS/MS (GC‐MS/MS), and thin layer chromatography (TLC) are conventional methods for ZEN detection . Although these methods have high sensitivity and reliability, they require complex pretreatments, long analytical times, large solvent consumption, well‐trained operators, and expensive equipment .…”

Section: Introductionmentioning

confidence: 99%

Rapid and sensitive double‐label based immunochromatographic assay for zearalenone detection in cereals

Wei

Zhang

et al. 2018

Electrophoresis

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A double-label immunochromatographic based assay (DL-ICA) was developed to monitor zearalenone (ZEN) levels in cereals, based on Eu nanoparticles (EuNP). The DL-ICA exhibited excellent sensitivity, reliability and selectivity in real samples. It showed low limits of detection (0.21-0.25 μg/kg) and broad analytical ranges (up to 120 μg/kg). The total analytical time, including sample preparation and DL-ICA execution, was reduced by 15 min compared with HPLC. The recovery rates ranged from 95.0-118.4%, with relative standard deviations (RSD) <11.6%. Inter- and intra-day validations were assessed, recovery rates of 89.3-106.9% and RSD of 2.3-9.7% were obtained, suggesting considerable stability and reliability for the assay. An excellent correlation was observed between DL-ICA and a reference HPLC method (R = 0.9899). Compared to current immunoassays, the current DL-ICA is inexpensive, highly sensitive, and rapid. Therefore, DL-ICA constitutes a novel tool for monitoring mycotoxins in food and feed to ensure safety.

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A new amplified π-shape electrochemical aptasensor for ultrasensitive detection of aflatoxin B1

Cited by 114 publications

References 34 publications

A New Approach for Detection of Aflatoxin B1

A New Approach for Detection of Aflatoxin B1

A Novel Electrochemical Aptasensor for Carcinoembryonic Antigen Detection Based on Target‐induced Bridge Assembly

Rapid and sensitive double‐label based immunochromatographic assay for zearalenone detection in cereals

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