A new analytical system for quantification scratching behaviour in mice

Orito, Kensuke; Chida, Yoichi; Fujisawa, Chie; Arkwright, Peter D.; Matsuda, Hiroshi

doi:10.1111/j.1365-2133.2004.05744.x

Cited by 39 publications

(37 citation statements)

References 22 publications

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“…Increase in the number of scratching behavior and exacerbation of clinical skin symptoms were closely correlated, suggesting that development of scratching behavior may play a critical role in pathogenesis of atopic dermatitis [9]. Because scratching behavior results from itch [9,11], investigation concerning itch may provide a new aspect of the disease and may contribute much to establishment of novel therapy for atopic dermatitis. A chain of reaction at skin lesions may bring serious exacerbation of atopic dermatitis.…”

Section: Discussionmentioning

confidence: 99%

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Expression of Nerve Growth Factor in Itchy Skins in Atopic NC/NgaTnd Mice

Tanaka

Matsuda

2005

The Journal of Veterinary Medical Science

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ABSTRACT. Although the possible involvement of neurotrophic factors in itchy skins of atopic dermatitis has been predicted, the exact mechanism by which itch is induced remains unclear. Since nerve growth factor (NGF) has crucial effects on development and functions of sensory nerves, we determined production of NGF and extension of nerve fibers in skins of NC/NgaTnd mice with or without atopic dermatitis. NC/NgaTnd mice spontaneously develop atopic dermatitis-like skin lesions when they are raised in air-unregulated conventional circumstances. We quantified scratching behavior of NC/NgaTnd mice during the development of dermatitis using a novel analytical system and compared to clinical skin severity scores. A significant correlation between the severity of dermatitis and the increase in the number of scratches was identified, indicating that scratching behavior may associate with clinical skin conditions. NGF contents in the skin lesions of conventional NC/NgaTnd mice were significantly higher than those in SPF mice. Positive reactions for NGF were observed in keratinocytes and fibroblasts in affected skins of conventional NC/NgaTnd mice. Immunohistochemical analysis showed the extension of protein gene product 9.5-positive nerve fibers from the dermis toward the epidermis at the skin lesions. These results suggest that sensory nerves induced by NGF may contribute to development of itch, and that NGF produced at the affected site may provide abnormal skin sensitivity in atopic dermatitis. KEY WORDS: itch, keratinocyte, mouse model, nerve fiber, neurotorophic factor.J. Vet. Med. Sci. 67(9): 915-919, 2005

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Section: Discussionmentioning

confidence: 99%

“…Scratching behavior of the mice was recorded and analyzed using a SCLABA TM system [11] (Noveltec Inc., Kobe, Japan) at indicated age of weeks according to the manufacture's instructions.…”

Section: Methodsmentioning

confidence: 99%

Expression of Nerve Growth Factor in Itchy Skins in Atopic NC/NgaTnd Mice

Tanaka

Matsuda

2005

The Journal of Veterinary Medical Science

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“…Ointment was then applied, and the number and duration of scratching sessions were counted according to the manufacturer's protocol for 15 minutes. 20 …”

Section: Scratching Behaviormentioning

confidence: 99%

Flaky Tail Mouse Denotes Human Atopic Dermatitis in the Steady State and by Topical Application with Dermatophagoides pteronyssinus Extract

Moniaga

Egawa

Kawasaki

et al. 2010

The American Journal of Pathology

121

126

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“…Scratching frequency and its duration were recorded on videotape for 20 min using a digital video camera (GR-DV700, JVC, Tokyo, Japan). Data were analyzed as previously described and validated in our laboratory [11]. …”

Section: Methodsmentioning

confidence: 99%

Oral Administration of Bepotastine Besilate Suppressed Scratching Behavior of Atopic Dermatitis Model NC/Nga Mice

Tanizaki

Kambe

Nakamura

et al. 2007

Int Arch Allergy Immunol

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Background: Pruritus is the most severe problem in atopic dermatitis. Even though its mechanism is still not fully understood, antihistamines have been prescribed for atopic dermatitis. Objective: To evaluate the effect of antihistamine on atopic dermatitis, we analyzed the scratching behavior in atopic dermatitis model NC/Nga mice. Methods: BALB/c mice, in which scratching behavior was induced by intradermal injection of compound 48/80 (100 µg/100 µl/mouse), and NC/Nga mice, housed in a conventional environment and having developed spontaneous eczematous regions, were monitored with a SCLABA system after oral administration of bepotastine besilate. The number of eosinophils in the ear skin and the serum leukotriene B₄ (LTB₄) levels were also evaluated. Results: Bepotastine at doses of 3 and 10 mg/kg effectively inhibited the compound 48/80-induced scratching behavior of BALB/c mice 1 h after oral administration, comparable with the blood T_max, which was reached within 0.8–1.6 h in humans. Bepotastine also significantly inhibited the scratching behavior of NC/Nga mice 1 h after oral administration. Even though 10 mg/kg bepotastine could not influence the number of tissue eosinophils, it effectively suppressed the serum LTB₄ levels, just comparable with the suppression of scratch behavior of NC/Nga mice. Conclusion: Bepotastin effectively suppressed the scratch behavior of atopic dermatitis model mice, which may not simply be explained by the suppression of histamine but also by the suppression of other mediators like LTB₄. Bepotastine could be useful in the treatment of pruritus, especially early after oral administration.

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A new analytical system for quantification scratching behaviour in mice

Abstract: This system provides a new method of quantifying scratching behaviour in a mouse.

Cited by 39 publications

References 22 publications

Expression of Nerve Growth Factor in Itchy Skins in Atopic NC/NgaTnd Mice

Expression of Nerve Growth Factor in Itchy Skins in Atopic NC/NgaTnd Mice

Flaky Tail Mouse Denotes Human Atopic Dermatitis in the Steady State and by Topical Application with Dermatophagoides pteronyssinus Extract

Oral Administration of Bepotastine Besilate Suppressed Scratching Behavior of Atopic Dermatitis Model NC/Nga Mice

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