A New and Sensitive Catalytic Resonance Scattering Spectral Assay for the Detection of Laccase Activity Using H2O2-I−-TDMAC System

Abstract

In pH 3.8 acetic acid-sodium acetate (HAC-NaAC) buffer solution, laccase exhibited a strong catalytic effect on the H 2 O 2 oxidation of I -to form I 2 , and I 2 combined with excess I -to form 3 I -that reacted with cationic surfactants of tetradecyl dimethylbenzyl ammonium chloride (TDMAC) to produce the (TDMAC-I 3 ) n association complex particles, which exhibited a strong resonance scattering (RS) peak at 468 nm. Under the chosen conditions, as the concentration of laccase activity increased, the RS intensity at 468 nm (I 468 nm ) increased linearly. The increased RS intensity ∆I 468 nm was linear to laccase activity in the range of 0.08-0.96 U/mL, with a regression equation of ∆I 468 nm ＝88.8U-1.9, and a detection limit of 0.02 U/mL laccase. This proposed method was applied to detect laccase activity in waste water, with satisfactory results.

A sensitive enzyme-catalytic nanogold-resonance scattering spectral assay for alkaline phosphate

“…However, determination of protein mostly based on simple intermolecular interactions between protein molecules and small molecules of dyestuff, the selectivity should be improved. Enzymatic reaction exhibited highly selective and enlarge analysis signal, it was combined with Rayleigh light scattering technique to develop some enzymatic-resonance scattering spectral assays [7][8][9][10]. Based on the catalysis of laccase to H 2 O 2 -KI-cationic surfactant system, an enzyme-catalytic RS assay was proposed for the determining laccase.…”

The formation of zirconium hexacyanoferrate(ii) nanoparticles and their application in the highly sensitive determination of dopamine based on enhanced resonance Rayleigh scattering

“…Moreover, it is also used for the investigation of phase separation, chain motions and microstructure changes of polymers, [43][44][45] and for the study of associated complex particle systems. 46,47 In addition, as nanotechnology develops rapidly, using nanoparticles and quantum dots as a novel probe in the RRS technique has attracted tremendous attention from scientic communities. [48][49][50][51][52] Nowadays, the RRS technique is gradually being used to study system reaction mechanisms.…”