A new angle on dynamic depolarized light scattering: number-averaged size distribution of nanoparticles in focus

Geers, Christoph; Rodríguez‐Lorenzo, Laura; Urban, Dominic A.; Kinnear, Calum; Petri‐Fink, Alke; Balog, Sandor

doi:10.1039/c6nr03386e

Cited by 24 publications

(16 citation statements)

References 61 publications

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“…The Smoluchowski approximation was fitted to 10 cycles of electrophoretic mobility (EPM) measurement and 10 measurements were obtained for each sample to estimate the mean and standard deviation (SD). Hydrodynamic radii were measured by depolarized dynamic light scattering (DDLS) and the values have been provided as intensity-weighted size distributions as previously reported by Geers et al [11]. Briefly, samples were diluted into CCM and measurements were carried out at 37 • C with a scattering angle of 30 • .…”

Section: Characterization Methodsmentioning

confidence: 99%

Quantification of Carbon Nanotube Doses in Adherent Cell Culture Assays Using UV-VIS-NIR Spectroscopy

et al. 2019

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The overt hazard of carbon nanotubes (CNTs) is often assessed using in vitro methods, but determining a dose–response relationship is still a challenge due to the analytical difficulty of quantifying the dose delivered to cells. An approach to accurately quantify CNT doses for submerged in vitro adherent cell culture systems using UV-VIS-near-infrared (NIR) spectroscopy is provided here. Two types of multi-walled CNTs (MWCNTs), Mitsui-7 and Nanocyl, which are dispersed in protein rich cell culture media, are studied as tested materials. Post 48 h of CNT incubation, the cellular fractions are subjected to microwave-assisted acid digestion/oxidation treatment, which eliminates biological matrix interference and improves CNT colloidal stability. The retrieved oxidized CNTs are analyzed and quantified using UV-VIS-NIR spectroscopy. In vitro imaging and quantification data in the presence of human lung epithelial cells (A549) confirm that up to 85% of Mitsui-7 and 48% for Nanocyl sediment interact (either through internalization or adherence) with cells during the 48 h of incubation. This finding is further confirmed using a sedimentation approach to estimate the delivered dose by measuring the depletion profile of the CNTs.

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Section: Characterization Methodsmentioning

confidence: 99%

Quantification of Carbon Nanotube Doses in Adherent Cell Culture Assays Using UV-VIS-NIR Spectroscopy

et al. 2019

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“…(5) is dependent on P(r); hence, fitting an analytic function requires a parametric model distribution that adequately describes the true particle size distribution. While models are practical in certain situations (Geers et al 2016;Rodriguez-Lorenzo et al 2016), they are not always available or represent real scenarios, and thus the true shape of P(r) frequently remains unknown. To circumvent this limitation, we apply here a more general, model-independent, and accurate non-parametric description.…”

Section: Theorymentioning

confidence: 99%

Taylor dispersion of nanoparticles

et al. 2017

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The ability to detect and accurately characterize particles is required by many fields of nanotechnology, including materials science, nanotoxicology, and nanomedicine. Among the most relevant physicochemical properties of nanoparticles, size and the related surface-to-volume ratio are fundamental ones. Taylor dispersion combines three independent phenomena to determine particle size: optical extinction, translational diffusion, and sheer-enhanced dispersion of nanoparticles subjected to a steady laminar flow. The interplay of these defines the apparent size. Considering that particles in fact are never truly uniform nor monodisperse, we rigorously address particle polydispersity and calculate the apparent particle size measured by Taylor dispersion analysis. We conducted case studies addressing aqueous suspensions of model particles and large-scaleproduced Bindustrial^particles of both academic and commercial interest of various core materials and sizes, ranging from 15 to 100 nm. A comparison with particle sizes determined by transmission electron microscopy confirms that our approach is model-independent, nonparametric, and of general validity that provides an accurate account of size polydispersity-independently on the shape of the size distribution and without any assumption required a priori.

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“…Furthermore, the area of detection in LIT is not limited to a small sample volume fraction, the method is nonintrusive and the sample can be recovered after the analysis. Considering that only the light absorbed by the NPs is converted into heat differentiates LIT from light‐scattering techniques, as the main advantage of LIT relies on the flexibility to adapt the applied trigger signal to match with the plasmonic resonance of the NPs. In addition, only very low amounts of sample material (40 µL per sample were used in this study) are needed to perform a LIT measurement, representing an advantage over common UV‐Vis spectroscopy.…”

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Lock‐In Thermography to Analyze Plasmonic Nanoparticle Dispersions

Steinmetz

Taladriz‐Blanco

Geers

et al. 2019

Part & Part Syst Charact

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The physicochemical properties of nanoparticles (NPs) strongly rely on their colloidal stability, and any given dispersion can display remarkably different features, depending on whether it contains single particles or clusters. Thus, developing efficient experimental methods that are able to provide accurate and reproducible measures of the NP properties is a considerable challenge for both research and industrial development. By analyzing different NPs, through size and concentration, it is demonstrated that lock‐in thermography, based on light absorption and heat generation, is able to detect and differentiate the distinct aggregation and re‐dispersion behavior of plasmonic NPs, e.g., gold and silver. Most importantly, the approach is nonintrusive and potentially highly cost‐effective compared to standard analytical techniques.

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A new angle on dynamic depolarized light scattering: number-averaged size distribution of nanoparticles in focus

Cited by 24 publications

References 61 publications

Quantification of Carbon Nanotube Doses in Adherent Cell Culture Assays Using UV-VIS-NIR Spectroscopy

Quantification of Carbon Nanotube Doses in Adherent Cell Culture Assays Using UV-VIS-NIR Spectroscopy

Taylor dispersion of nanoparticles

Lock‐In Thermography to Analyze Plasmonic Nanoparticle Dispersions

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