A new antimicrobial peptide isoform, Pc-crustin 4 involved in antibacterial innate immune response in fresh water crayfish, Procambarus clarkii

Du, Zhiqiang; Li, Bo; Shen, Xiu-li; Wang, Kai; Du, Jie; Yu, Xiaodong; Yuan, Jianjun

doi:10.1016/j.fsi.2019.10.003

Cited by 23 publications

(12 citation statements)

References 32 publications

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“…As one kind of antimicrobial peptide, crustins are important components for the first line of host immune defense system. Most reported crustin genes are responsive to immune challenges of various pathogens [ 16 , 27 , 28 , 32 , 43 ]. Here, the widely distributed type VII crustin LvCrustinVII was responsive to V. parahaemolyticus infection in the hepatopancreas of L. vannamei .…”

Section: Discussionmentioning

confidence: 99%

“…They are featured with a C-terminal whey acidic protein (WAP) domain, which consists of about 50 amino acids and eight conserved cysteine residues that form a characteristic four-disulfide bonds (4DSC) [ 26 ]. The WAP domain is a key structural basis for its biological activity [ 27 ]. In addition, the amino acid polytropic region located between the signal peptide sequence and the WAP domain is important for its functions [ 28 ].…”

Section: Introductionmentioning

confidence: 99%

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Characterization of the Dual Functions of LvCrustinVII from Litopenaeus vannamei as Antimicrobial Peptide and Opsonin

et al. 2022

Marine Drugs

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Crustin are a family of antimicrobial peptides that play an important role in protecting against pathogens infection in the innate immune system of crustaceans. Previously, we identified several novel types of crustins, including type VI and type VII crustins. However, their immune functions were still unclear. In the present study, the immune function of type VII crustin LvCrustinVII were investigated in Litopenaeus vannamei. LvCrustinVII was wildly expressed in all tested tissues, with relatively high expression levels in hepatopancreas, epidermis and lymphoid organ. Upon Vibrio parahaemolyticus infection, LvCrustinVII was significantly upregulated in hepatopancreas. Recombinant LvCrustinVII (rLvCrustinVII) showed strong inhibitory activities against Gram-negative bacteria Vibrio harveyi and V. parahaemolyticus, while weak activities against the Gram-positive bacteria Staphylococcus aureus. Binding assay showed that rLvCrustinVII could bind strongly to V. harveyi and V. parahaemolyticus, as well as the cell wall components Glu, LPS and PGN. In the presence of Ca2+, rLvCrustinVII could agglutinate V. parahaemolyticus and enhance hemocyte phagocytosis. The present data partially illustrate the immune function of LvCrustinVII, which enrich our understanding on the functional mechanisms of crustins and provide useful information for application of this kind of antimicrobial peptides.

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Section: Discussionmentioning

confidence: 99%

Section: Introductionmentioning

confidence: 99%

Characterization of the Dual Functions of LvCrustinVII from Litopenaeus vannamei as Antimicrobial Peptide and Opsonin

et al. 2022

Marine Drugs

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“…Crustins show broad-spectrum activity against a range of microorganisms, including Aeromonas hydrophila [ 132 , 133 ], Edwarsiella ictaluri [ 134 ], E. coli [ 132 , 135 , 136 ], M. maritypicum [ 135 ], P. aeruginosa [ 135 , 136 ], and V. parahaemolyticus [ 133 , 136 ], among others. The antimicrobial activities of these peptides have been demonstrated, not only for free-living organisms, but also for those in biofilms.…”

Section: Defense Mechanisms In Crustaceansmentioning

confidence: 99%

Marine Arthropods as a Source of Antimicrobial Peptides

et al. 2022

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Peptide therapeutics play a key role in the development of new medical treatments. The traditional focus on endogenous peptides has shifted from first discovering other natural sources of these molecules, to later synthesizing those with unique bioactivities. This review provides concise information concerning antimicrobial peptides derived from marine crustaceans for the development of new therapeutics. Marine arthropods do not have an adaptive immune system, and therefore, they depend on the innate immune system to eliminate pathogens. In this context, antimicrobial peptides (AMPs) with unique characteristics are a pivotal part of the defense systems of these organisms. This review covers topics such as the diversity and distribution of peptides in marine arthropods (crustacea and chelicerata), with a focus on penaeid shrimps. The following aspects are covered: the defense system; classes of AMPs; molecular characteristics of AMPs; AMP synthesis; the role of penaeidins, anti-lipopolysaccharide factors, crustins, and stylicins against microorganisms; and the use of AMPs as therapeutic drugs. This review seeks to provide a useful compilation of the most recent information regarding AMPs from marine crustaceans, and describes the future potential applications of these molecules.

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“…AMPs are widely distributed small cationic polypeptides that exert an anti-pathogenic effect on a broad spectrum of pathogens. Crustacean AMPs can generally be classified into four groups according to their amino acid compositions and structural properties ( 106 , 127 ) ( 1 ): single-domain amphipathic linear α-helical structure AMPs, including astacidins which are proline-rich peptides like astacidin 2 from P. leniusculus ( 137 ), and Pc-astacidin from P. clarkii ( 127 ) ( 2 ); single-domain peptides enriched with cysteine residues that are able to form at least one disulfide bridge between the B-sheets ( 3 ); AMPs with multi-domain linear α-helical peptides enriched with certain amino acids, generally proline and/or glycine residues, including crustins, characterized by their acidic protein (WAP) domain at C-terminus, such as Pl-crustin 1 & 2 in P. leniusculus , and Pc-crustin 4 in P. clarkii ( 138 , 139 ), and procambarins, the glycine-rich peptide described in P. clarkia ( 140 ); and ( 4 ) unconventional AMPs including proteins/protein-fragments with antimicrobial functions like H2A histones and anti-lipopolysaccharide factors (ALFs) (reviewed in 121, 126). Lastly, at least two types of invertebrate (i-types) lysozymes, Pclysi 1 and 2, have been identified in P. clarkii ( 141 ).…”

Section: Molecular Components Of Crayfish Immunitymentioning

confidence: 99%

A Comprehensive Review on Crustaceans’ Immune System With a Focus on Freshwater Crayfish in Relation to Crayfish Plague Disease

Bouallegui

2021

Front. Immunol.

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Freshwater crayfish immunity has received great attention due to the need for urgent conservation. This concern has increased the understanding of the cellular and humoral defense systems, although the regulatory mechanisms involved in these processes need updating. There are, however, aspects of the immune response that require clarification and integration. The particular issues addressed in this review include an overall description of the oomycete Aphanomyces astaci, the causative agent of the pandemic plague disease, which affects freshwater crayfish, and an overview of crustaceans’ immunity with a focus on freshwater crayfish. It includes a classification system of hemocyte sub-types, the molecular factors involved in hematopoiesis and the differential role of the hemocyte subpopulations in cell-mediated responses, including hemocyte infiltration, inflammation, encapsulation and the link with the extracellular trap cell death pathway (ETosis). In addition, other topics discussed include the identity and functions of hyaline cells, the generation of neoplasia, and the emerging topic of the role of sessile hemocytes in peripheral immunity. Finally, attention is paid to the molecular execution of the immune response, from recognition by the pattern recognition receptors (PRRs), the role of the signaling network in propagating and maintaining the immune signals, to the effector elements such as the putative function of the Down syndrome adhesion molecules (Dscam) in innate immune memory.

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A new antimicrobial peptide isoform, Pc-crustin 4 involved in antibacterial innate immune response in fresh water crayfish, Procambarus clarkii

Cited by 23 publications

References 32 publications

Characterization of the Dual Functions of LvCrustinVII from Litopenaeus vannamei as Antimicrobial Peptide and Opsonin

Characterization of the Dual Functions of LvCrustinVII from Litopenaeus vannamei as Antimicrobial Peptide and Opsonin

Marine Arthropods as a Source of Antimicrobial Peptides

A Comprehensive Review on Crustaceans’ Immune System With a Focus on Freshwater Crayfish in Relation to Crayfish Plague Disease

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