A new antimicrobial protein from the anterior midgut of Triatoma infestans mediates Trypanosoma cruzi establishment by controlling the microbiota

Buarque, Diego S.; Gomes, Cícera M.; Araújo, Ricardo Nascimento; Pereira, Marcos H.; Ferreira, Roberta Carvalho; Guarneri, Alessandra A.; Tanaka, Aparecida S.

doi:10.1016/j.biochi.2016.02.009

Cited by 31 publications

(26 citation statements)

References 36 publications

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“…The triatomine immune system may play a role in these interactions. After blood feeding, and in insects fed with T. cruzi, T. rangeli, or bacteria, there is an activation of immune pathway genes, increases in AMP gene expression levels, increased levels of effector proteins and differential antimicrobial activity [246,247,249,255,[259][260][261][262][263][264][265][266]. The AMPs involved in these studies are likely controlled by the Toll and IMD pathways as silencing the NF-κB transcription factor relish (IMD pathway) reduces the intestinal bacterial population while the opposite occurs when the NF-κB transcription factor dorsal (Toll pathway) is silenced [251].…”

Section: Effects Of Trypanosomes On the Microbiotamentioning

confidence: 99%

The Role of Bacterial Symbionts in Triatomines: An Evolutionary Perspective

et al. 2020

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Insects have established mutualistic symbiotic interactions with microorganisms that are beneficial to both host and symbiont. Many insects have exploited these symbioses to diversify and expand their ecological ranges. In the Hemiptera (i.e., aphids, cicadas, and true bugs), symbioses have established and evolved with obligatory essential microorganisms (primary symbionts) and with facultative beneficial symbionts (secondary symbionts). Primary symbionts are usually intracellular microorganisms found in insects with specialized diets such as obligate hematophagy or phytophagy. Most Heteroptera (true bugs), however, have gastrointestinal (GI) tract extracellular symbionts with functions analogous to primary endosymbionts. The triatomines, are vectors of the human parasite, Trypanosoma cruzi. A description of their small GI tract microbiota richness was based on a few culturable microorganisms first described almost a century ago. A growing literature describes more complex interactions between triatomines and bacteria with properties characteristic of both primary and secondary symbionts. In this review, we provide an evolutionary perspective of beneficial symbioses in the Hemiptera, illustrating the context that may drive the evolution of symbioses in triatomines. We highlight the diversity of the triatomine microbiota, bacterial taxa with potential to be beneficial symbionts, the unique characteristics of triatomine-bacteria symbioses, and the interactions among trypanosomes, microbiota, and triatomines.

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Section: Effects Of Trypanosomes On the Microbiotamentioning

confidence: 99%

The Role of Bacterial Symbionts in Triatomines: An Evolutionary Perspective

et al. 2020

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“…Triatomines harbor a diverse gut microbiome that is beginning to be identified 30 , 31 , but its influence on T. cruzi transmission remains unclear. In Triatoma infestans , infection by T. cruzi induces the overexpression of antimicrobial peptides modulating the microbiome, and this inhibition of the bacterial microflora is important for parasite establishment in the vector 32 . Nonetheless, our understanding of triatomine microbiome is still too limited to further assess its role in vectorial capacity and T. cruzi transmission, or to take full advantage of its potential manipulation in novel parasite control strategies such as para-transgenesis as suggested before 33 , 34 .…”

Section: Introductionmentioning

confidence: 99%

Detailed ecological associations of triatomines revealed by metabarcoding and next-generation sequencing: implications for triatomine behavior and Trypanosoma cruzi transmission cycles

Dumonteil¹,

Ramírez-Sierra²,

Pérez-Carrillo³

et al. 2018

Sci Rep

115

151

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Trypanosoma cruzi is the agent of Chagas disease, transmitted by hematophagous triatomine vectors. Establishing transmission cycles is key to understand the epidemiology of the disease, but integrative assessments of ecological interactions shaping parasite transmission are still limited. Current approaches also lack sensitivity to assess the full extent of this ecological diversity. Here we developed a metabarcoding approach based on next-generation sequencing to identify triatomine gut microbiome, vertebrate feeding hosts, and parasite diversity and their potential interactions. We detected a dynamic microbiome in Triatoma dimidiata, including 23 bacterial orders, which differed according to blood sources. Fourteen vertebrate species served as blood sources, corresponding to domestic, synantropic and sylvatic species, although four (human, dog, cow and mice) accounted for over 50% of blood sources. Importantly, bugs fed on multiple hosts, with up to 11 hosts identified per bug, indicating very frequent host-switching. A high clonal diversity of T. cruzi was detected, with up to 20 haplotypes per bug. This analysis provided much greater sensitivity to detect multiple blood meals and multiclonal infections with T. cruzi, which should be taken into account to develop transmission networks, and characterize the risk for human infection, eventually leading to a better control of disease transmission.

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“…The bacterial microbiome living in the gastrointestinal tract or other organs of a host has increasingly been investigated as a potential target for intervention in efforts to limit disease transmission by a variety of arthropod vectors (Cirimotich et al, 2011;Crotti et al, 2012;Geiger et al, 2013;Hegde et al, 2015;Taracena et al, 2015;Vieira et al, 2015;Buarque et al, 2016). To date, few studies have examined the gut microbiome of triatomine vectors, and even fewer have looked at Triatoma spp.…”

Section: Introductionmentioning

confidence: 99%