1995
DOI: 10.1002/bio.1170100203
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A new approach for analysis of chemiluminescent kinetics of activated phagocytes in blood

Abstract: Luminol chemiluminescence (LCL) is a simple, sensitive and time-saving tool to elucidate the oxidative activity of polymorphonuclear leukocytes (PMNL). In this study, a new approach for analysing the LCL kinetics, recorded from stimulated PMNL, and for a more accurate elucidation of their functional state, is proposed. This approach is based on the proposal that the LCL kinetics of stimulated PMNL is a result of time-probabilistic nature of the processes, leading to light emission. On this basis the LCL respon… Show more

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Cited by 9 publications
(7 citation statements)
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“…Surprisingly, reviews of immune effector mechanisms against blood stage Plasmodium infection rarely mention or highlight roles for neutrophils or ROS [20], [21]. Neutrophil respiratory bursts can be quantified by emitted light detected with chemiluminescence dyes [22][25], and can be triggered by opsonized P. falciparum merozoites, and enhanced by cytokines [26]. Large scale functional analysis of anti-merozoite antibodies using this approach, has been limited by a lack of miniaturized and/or automated procedures [13], [26]–[28].…”
Section: Introductionmentioning
confidence: 99%
“…Surprisingly, reviews of immune effector mechanisms against blood stage Plasmodium infection rarely mention or highlight roles for neutrophils or ROS [20], [21]. Neutrophil respiratory bursts can be quantified by emitted light detected with chemiluminescence dyes [22][25], and can be triggered by opsonized P. falciparum merozoites, and enhanced by cytokines [26]. Large scale functional analysis of anti-merozoite antibodies using this approach, has been limited by a lack of miniaturized and/or automated procedures [13], [26]–[28].…”
Section: Introductionmentioning
confidence: 99%
“…The recorded kinetic CL curves were presented as a sum of three biological components representing the following processes: , (1) extracellular ROS generation connected to phagocytosis; (2) intracellular ROS generation, connected to phagocytosis; (3) intracellular ROS, not connected to phagocytosis. To decompose the chemiluminescent curve into the three aforementioned components, the PeakFit program was employed, using Poisson-type distribution equations for each component, considering the boundary conditions for the time appearance of each peak: first component t 1 [1–6] min, second component t 2 [7–13], third component t 3 [>13] min. The first and the third components were decomposed arbitrarily to two additional components to represent the CL multimodal curve in more details: t 1 .25em false[ 1−6 false] nobreak.25em min = t 1 _ 1 false[ 1−2 false] .25em min nobreak.25em + t 1 _ 2 .25em false[ 3−6 false] .25em min t 3 .25em false[ >13 false] .25em min nobreak.25em = t 3 _ 1 .25em false[ 13−25 false] .25em min nobreak.25em + t 3 _ 2 .25em false[ >26 false] .25em min …”
Section: Methodsmentioning
confidence: 99%
“…The CL kinetics were simultaneously recorded from a luminometer in a photon-counting mode (15). Each recorded kinetic CL curve was presented as a sum of three statistical (Poisson-type) distributions (12). These non-linear model components were computer-designed, using the SYSTAT system (see Appendix).…”
Section: Assaysmentioning
confidence: 99%
“…4B). The temperature of the samples (12), and the amount of luminol (unpublished data) also change the relative contribution of the components of CL kinetics, thus changing the estimation of CEV coordinates and state of PMNs.…”
Section: Relativity Of the Pmn Statesmentioning
confidence: 99%
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