A new approach for measurement of cytotoxicity using colorimetric assay

Hussain, R.F.; Nouri, A. M. E.; Oliver, R.T.D.

doi:10.1016/0022-1759(93)90012-v

Cited by 303 publications

(150 citation statements)

References 9 publications

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“…33,35 Cytotoxicity values obtained using the tetrazolium-based colorimetric assay has been shown to strongly correlate with results of the 51 Cr release assay. 36,37 SK-BR3 and MDA-MB231 cells were seeded in 96 well plates at 1 3 10 4 cells/well and allowed to adhere at 37°C overnight. Vg9Vd2 T cells were added at different E:T ratios in the presence or absence of trastuzumab (2 lg/ml) or human IgG (2 lg/ml).…”

Section: Cytotoxicity Assessment Against Adherent Tumor Cell Lines (Mmentioning

confidence: 99%

Vγ9Vδ2 T cell cytotoxicity against tumor cells is enhanced by monoclonal antibody drugs—Rituximab and trastuzumab

Tokuyama

Hagi

Mattarollo

et al. 2008

Intl Journal of Cancer

134

121

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Vc9Vd2 T cells exert potent cytotoxicity toward various tumor cells and adoptive transfer of Vc9Vd2 T cells is an attractive proposition for cell based immunotherapy. Vc9Vd2 T cells expanded in the presence of Zoledronate and IL-2 express CD16 (FccRIII), which raises the possibility that Vc9Vd2 T cells could be used in conjunction with tumor targeting monoclonal antibody drugs to increase antitumor cytotoxicity by antibody dependent cellular cytotoxicity (ADCC). Cytotoxic activity against CD20-positive B lineage lymphoma or chronic lymphocytic leukemia (CLL) and HER2-positive breast cancer cells was assessed in the presence of rituximab and trastuzumab, respectively. Cytotoxicity of Vc9Vd2 T cells against CD20-positive targets was higher when used in combination with rituximab. Similarly, Vc9Vd2 T cells used in combination with trastuzumab resulted in greater cytotoxicity against HER2-positive cells in comparison with either agent alone and this effect was restricted to the CD16 1 Vc9Vd2 T cell population. Our results show that CD16 1 Vc9Vd2 T cells recognize monoclonal antibody coated tumor cells via CD16 and exert ADCC similar to that observed with NK cells, even when target cells are relatively resistant to monoclonal antibodies or Vc9Vd2 T cells alone. Combination therapy involving ex vivo expanded CD16 1 Vc9Vd2 T cells and monoclonal antibodies may enhance the clinical outcomes for patients treated with monoclonal antibody therapy. '

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Section: Cytotoxicity Assessment Against Adherent Tumor Cell Lines (Mmentioning

confidence: 99%

Vγ9Vδ2 T cell cytotoxicity against tumor cells is enhanced by monoclonal antibody drugs—Rituximab and trastuzumab

Tokuyama

Hagi

Mattarollo

et al. 2008

Intl Journal of Cancer

134

121

View full text Add to dashboard Cite

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“…MTT reduction assay: Cellular viability was assayed with MTT method [31,32]. In the assay, different concentrations of samples (100 µl) were applied to the wells of 96-well plate containing confluent cell monolayer in triplicate, while the dilution medium without the sample was used as the control.…”

Section: Plaque Reduction Assaymentioning

confidence: 99%

Esterification of the Free Carboxylic Group in 3,4-di-O-caffeoylquinic Acid Enhances the Inhibition Activity against Respiratory Syncytial Virus (RSV)

Wu¹,

Tang²

2015

Med chem

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Based on molecular docking analysis, nine alkyl 3,4-di-O-caffeoyl-quinates have been designed. Started from 3,4-di-O-caffeoylquinic acid (3,4-DCQA), all the compounds have been synthesized using thionylchloride as coupling reagent with yields of 56%-72%. By applying plaque reduction assay, the anti-respiratory syncytial virus (RSV) activities of all the compounds were evaluated. The IC 50 values of all the derivatives were 2.9 to 7.8 times less than that of 3,4-DCQA itself. Isopropyl 3,4-Di-O-caffeoyl quinate (LS-4) was confirmed as the most active compound with IC 50 value of 0.3 µM. It was indicated that hydrophobic groups with 2 to 4 carbon chain length were optimal for the enhancement of anti-RSV activity. Interestingly, all the derivatives showed greater cytotoxicity than 3,4-DCQA. Except LS-5, LS-6 and LS-9, all the derivatives have less toxicity than ribavirin. Furthermore, the stabilities of LS-4 in water, artificial gastric juice (AGJ), and artificial intestinal juice were evaluated, respectively. It was shown that LS-4 is stable in AGJ with the hydrolysis rate of 24.9% after 6 hours incubation at 37°C.

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“…Cell viability was determined by the MTT assay (14). Briefly, aliquots of HL-60 cells containing 1 x 10 5 cells/mL were added to each well of a 96-well flat-microtiter plate and incubated with various doses of fraction FA-2-b-ß (0, 5, 10, 20, 40, and 80 µg/mL).…”

Section: Cell Viabilitymentioning

confidence: 99%

Primary mechanism of apoptosis induction in a leukemia cell line by fraction FA-2-b-ß prepared from the mushroom Agaricus blazei Murill

Gao¹,

Sun²,

Chen³

et al. 2007

Braz J Med Biol Res

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Agaricus blazei Murill is a native Brazilian mushroom which functions primarily as an anticancer substance in transplanted mouse tumors. However, the mechanism underlying this function of A. blazei Murill remains obscure. The present study was carried out to investigate the effect of fraction FA-2-b-ß, an RNA-protein complex isolated from A. blazei Murill, on human leukemia HL-60 cells in vitro. Typical apoptotic characteristics were determined by morphological methods using DNA agarose gel electrophoresis and flow cytometry. The growth suppressive effect of fraction FA-2-b-ß on HL-60 cells in vitro occurred in a dose-(5-80 µg/mL) and time-dependent (24-96 h) manner. The proliferation of HL-60 cells (1 x 10 5 cells/mL) treated with 40 µg/mL of fraction FA-2-b-ß for 24-96 h and with 5-80 µg/mL for 96 h resulted in inhibitory rates ranging from 8 to 54.5%, and from 4.9 to 86.3%, respectively. Both telomerase activity determined by TRAP-ELISA and mRNA expression of the caspase-3 gene detected by RT-PCR were increased in HL-60 cells during fraction FA-2-b-ß treatment. The rate of apoptosis correlated negatively with the decrease of telomerase activity (r = 0.926, P < 0.05), but correlated positively with caspase-3 mRNA expression (r = 0.926, P < 0.05). These data show that fraction FA-2-b-ß can induce HL-60 cell apoptosis and that the combined effect of down-regulation of telomerase activity and up-regulation of mRNA expression of the caspase-3 gene could be the primary mechanism of induction of apoptosis. These findings provide strong evidence that fraction FA-2-b-ß could be of interest for the clinical treatment of acute leukemia. Correspondence

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A new approach for measurement of cytotoxicity using colorimetric assay

Cited by 303 publications

References 9 publications

Vγ9Vδ2 T cell cytotoxicity against tumor cells is enhanced by monoclonal antibody drugs—Rituximab and trastuzumab

Vγ9Vδ2 T cell cytotoxicity against tumor cells is enhanced by monoclonal antibody drugs—Rituximab and trastuzumab

Esterification of the Free Carboxylic Group in 3,4-di-O-caffeoylquinic Acid Enhances the Inhibition Activity against Respiratory Syncytial Virus (RSV)

Primary mechanism of apoptosis induction in a leukemia cell line by fraction FA-2-b-ß prepared from the mushroom Agaricus blazei Murill

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