A New Approach for the Analysis of Testicular Cells Using a Laser Scanning Cytometer

Abstract

The laser scanning cytometer (LSC) is a new laboratory tool that offers increased [2,6,12]. The technology is similar to FC in that the measurements are based on the analysis of laser-excited fluorescence emitted by individual cells and detected by a scanner at different wavelengths [1,6,9]. It allows morphological evaluation of events gated according to fluorescence intensity with light and/or fluorescence microscopy [6]. In this paper, the application of LSC to the evaluation of spermatogenesis is described.Testicular cells were isolated from C57BL/6J mice (CLEA Japan, Tokyo, Japan) according to the Kwon Medicine, Chonbuk National University, Jeonju, Korea Analysis of biochemical events and the genes expressed at various postnatal developmental stages in the testis of mammals is great importance for understanding spermatogenesis [8].Mammalian spermatogenesis, the process of male gamete production, is a complex, highly organized process that can be divided into three main stages [7,10]: 1) mitotic proliferation of spermatogonial stem cells and premeiotic differentiation of primary spermatocytes; 2) progression of meiotic spermatocytes to haploid round spermatids via two successive divisions; and 3) spermiogenesis, a cellular and nuclear reorganization process that turns spermatids into spermatozoa.A major limitation in the study of genes and factors involved in germ cell differentiation has been the lack of an efficient in vitro system that supports this differentiation process [8,13]. Flow cytometry (FC) shows great potential for application to the assessment of sper-

High throughput FRET analysis of protein–protein interactions by slide‐based imaging laser scanning cytometry

“…LSC is capable of relocalizing cells allowing, e.g., monitoring the same cells before and after a treatment or following the time course of processes. LSC has been found useful to analyze various cellular processes ranging from enzyme reaction kinetics to apoptotic DNA damage (30)(31)(32)(33).…”