A new approach of stress evaluation in fish using β-d-Glucose measurement in fish holding-water

Makaras, Tomas; Razumienė, Julija; Gureviciene, Vidute; Šakinytė, Ieva; Stankevičiūtė, Milda; Kazlauskienė, Nijolė

doi:10.1016/j.ecolind.2019.105829

Cited by 22 publications

(8 citation statements)

References 44 publications

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“…Temperature dependencies of the chemical exchange rates were parameterized with the Eyring equation 3 at two (and partly also at a third) pH points, using the exchange rates from the MCMC fit of the restricted model. Whereas for pH = 6.21, spectra up to a temperature of 295 K could be used ( Fig.…”

Section: Resultsmentioning

confidence: 99%

See 1 more Smart Citation

Glucose exchange parameters in a subset of physiological conditions

Mareš,

Karjalainen,

Håkansson

et al. 2023

Phys. Chem. Chem. Phys.

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Section: Resultsmentioning

confidence: 99%

“… 2 Application can also be expected in animal production, such as salmon farming. 3 In order to measure the spatial distribution of glucose in the living body, the exchanging protons of the –OH sites can be used to create a natural contrast. The chemical exchange creates various effects in NMR, and these can be observed using e.g.…”

Section: Introductionmentioning

confidence: 99%

Glucose exchange parameters in a subset of physiological conditions

Mareš,

Karjalainen,

Håkansson

et al. 2023

Phys. Chem. Chem. Phys.

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“…The nutritional status and stress level of fish can be assessed through their blood glucose levels, hence accurate detection of the glucose content in fish blood is important [ 2 ]. Currently, common methods for determining monosaccharides such as glucose in food include the high-performance liquid chromatography–evaporative light scattering detection (HPLC-ELSD) method [ 3 , 4 , 5 ], the high-performance liquid chromatography–refractive index detection (HPLC-RID) method [ 6 , 7 , 8 ], the biosensor method [ 9 ], the nanowire aerogel method [ 10 ], and the glucose assay kit method [ 11 ]. Among them, the HPLC-ELSD method is classical and widely used for glucose detection, with well-developed procedures.…”

Section: Introductionmentioning

confidence: 99%

Using Magnetic Molecularly Imprinted Polymer Technology for Determination of Fish Serum Glucose Levels

Yao,

Gu,

Huang

et al. 2024

Polymers

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In this study, a highly efficient magnetic molecularly imprinted polymer nanocomposite material was prepared using multi-walled carbon nanotubes as carriers. The characterization of the obtained nanocomposite material was conducted using Fourier transform infrared spectroscopy, a vibrating sample magnetometer, a thermogravimetric analyzer, a scanning electron microscope, and a transmission electron microscope. The adsorption properties of the nanocomposite material were evaluated through adsorption experiments, including static adsorption, dynamic adsorption, and selective recognition studies. The prepared nanocomposite material, serving as a selective adsorbent, was applied in magnetic solid-phase extraction. Subsequently, the derivatized samples were analyzed for glucose in fish serum using liquid chromatography–tandem mass spectrometry. Under optimal conditions, the detection limit was 0.30 ng/mL, the quantitation limit was 0.99 ng/mL, satisfactory spiked recovery rates were obtained, and the relative standard deviation was less than 1.1%. Using 2-deoxy-D-ribose as the template molecule and a structural analog of glucose allowed us to eliminate the potential template leakage in qualitative and quantitative analyses, effectively avoiding the issues of false positives and potential quantitative errors, compared to traditional methods. A method for detecting glucose levels in fish serum based on molecularly imprinted polymer technology has been successfully developed to determine the stress and health levels of fish.

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“…If the fish's blood glucose condition is not normal, the fish's life will be disrupted and can even cause death. Measurement of blood glucose levels can be used to diagnose stressed fish simply, effectively, and rapidly for a variety of stressors ( Sulmartiwi et al, 2013 ; Makaras et al, 2020 ). Based on the description above, we conducted a study in Blitar to determine the type and number of parasites and also blood glucose levels in koi carp.…”

Section: Introductionmentioning

confidence: 99%

Blood glucose profile as a rapid method for observing Koi carp (Cyprinus carpio) health status - case study of ectoparasites in Blitar, Indonesia

Afiyah

Fira

Santanumurti

et al. 2023

Rev. Bras. Parasitol. Vet.

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Assessment of fish health is one of the efforts of farmers in minimizing losses due to disease. Rapid tests on fish health can be done through blood observations. This study aimed to determine the blood glucose profile of koi carp due to ectoparasite infestation from the level of blood glucose. The results showed that reported parasites from Blitar’s koi carp were Trichodina, Dactylogyrus, Gyrodactylus, Myxobolus, Thelohanellus, Ichthyophthirius, and Argulus. Trichodina showed the highest prevalence (100%) in this case while Thelohanellus was the highest intensity level (93.8±16.3). The results of blood glucose level measurement based on parasite infestation levels showed no significant difference (p>0.05) though the health problems caused by parasites in light, medium or heavy infestation. This research also indicated that the blood glucose profile could be used as a rapid method to detect fish health caused by parasites. We suggest that other variables such as nutritional status, life stage or feeding must be conducted to ensure the glucose role in parasite identification as a rapid method for the future work.

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A new approach of stress evaluation in fish using β-d-Glucose measurement in fish holding-water

Cited by 22 publications

References 44 publications

Glucose exchange parameters in a subset of physiological conditions

Glucose exchange parameters in a subset of physiological conditions

Using Magnetic Molecularly Imprinted Polymer Technology for Determination of Fish Serum Glucose Levels

Blood glucose profile as a rapid method for observing Koi carp (Cyprinus carpio) health status - case study of ectoparasites in Blitar, Indonesia

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