A New Approach to the Determination of Protein‐bound Bilirubin Displacement and Its Applications

Coutinho, Claude B.; Lucek, Rudolph; Cheripko, Joyce A.; Kuntzman, R.

doi:10.1111/j.1749-6632.1973.tb20485.x

Cited by 15 publications

(6 citation statements)

References 18 publications

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“…it must be assumed that alteration in albumin conformation has occurred. Furthermore, other studies have shown that drugs can displace bilirubin from its high affinity site (Coutinho, Lucek, Cheripko & Kuntzman, 1973;Schiff, Chan & Stem, 1972).…”

Section: Pooled Cord Serummentioning

confidence: 99%

The effect of semisynthetic penicillins on the binding of bilirubin by neonatal serum.

Friedman¹,

Lewis²

1980

Brit J Clinical Pharma

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1. The effect of ampicillin, cloxacillin, flucloxacillin and sulphafurazole on bilirubin on bilirubin binding by pooled human umbilical cord serum and bovine serum albumin was studied in vitro using Sephadex gel filtration. 2. Sulphafurazole displaced bilirubin from binding; both cloxacillins displaced bilirubin from pooled cord serum but not bovine serum albumin. 4. No displacement of bilirubin by the cloxacillins from pooled cord serum could be detected at therapeutic plasma concentrations of these drugs. 5. Scatchard analysis of the interactions showed that displacement of bilirubin by these drugs occurred principally at the primary, high affinity, low capacity binding site.

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Section: Pooled Cord Serummentioning

confidence: 99%

The effect of semisynthetic penicillins on the binding of bilirubin by neonatal serum.

Friedman¹,

Lewis²

1980

Brit J Clinical Pharma

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“…The bilirubin concentration in the supernatant was estimated by direct spectrophotometry (1,17) in a Beckman DU 2 Spectrophotometer (460 nm). A correction was made for the interference due to haemoglobin (19).…”

Section: Analytical Proceduresmentioning

confidence: 99%

“…* · cytes in bilirubin binding. Studies on the interaction of bilirubin with substances in the bloodstream show that bilirubin has a great affinity for albumin; two molecules of bilirubin bind with one Material and Methods molecule of albumin in such a way that one bilirubin Preparation of cell suspensions molecule is much more firmly bound than the other ^ from ^^ human ^^ and from ^ ujnbilical (1)(2)(3). In addition to binding with albumin, bilirubin is O f nor mal human newborns were used with heparin as antibound by erythrocytes (4)(5)(6)(7)(8)(9).…”

Section: Introductionmentioning

confidence: 99%

Bilirubin Adsorption In Vitro by Foetal and Adult Human Erythrocytes

Gioia¹,

Serrani²,

Corchs³

1981

CCLM

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Adsorption of bilirubin in vitro by human foetal erythrocytes from umbilical cords was significantly greater than the binding by adult erythrocytes.A difference in binding capacity was detected at 0.6 mmol/1 bilirubin concentration (bilirubin/albumin ratio 2:1) immediately after mixing with the bilirubin solution.After 30 min incubation a further increase in bound bilirubin was found to be due only to binding in foetal cells.The results suggest that bilirubin from the medium was incorporated into at least two compartments in foetal erythrocytes.A possible role of human foetal erythrocytes in bilirubin distribution to tissues is stressed. Adsorption von Bilirubin in vitro an foetale und adulte Erythrocyten des MenschenZusammenfassung: Die Adsorption von Bilirubin in vitro durch foetale menschliche Erythrocyten aus dem Blut der Nabelschnur war bedeutend größer als die durch Erythrocyten von Erwachsenen. Dieser Unterschied konnte bei einer Bilirubinkonzentration von 0,6 mmol/1 im Inkubationsmedium (einer Lösurig von Albumin, Verhältnis Bilirubin/ Albumin 2:1) unmittelbar nach dein Suspendieren des Blutes in der Bilirubinlösung festgestellt werden.Nach 30 Minuten Inkubationszeit bei 37 °C erhöhte sich nur die Adsorption von Bilirubin an foetale Erythrocyten signifikant, während man im Gegenteil keine Veränderungen beim Bilirubin in Gegenwart der roten Blutkörperchen Erwachsener beobachten konnte. Die Ergebnisse deuten daraufhin, daß das Bilirubin des Inkubationsmediums in zwei Kompartimente der foetalen Erythrocyten aufgenommen wird. Die mögliche Rolle foetaler Erythrocyten bei der Verteilung des freien Bilirubins in den Geweben wäre demzufolge zu beachten.

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“…The significantly lower plasma binding of both iotroxamide and ioglycamide in the presence of jaundice probably reflects the fact that other substances in the patient's blood are competing for albumin binding sites. Bilirubin has been shown to displace a number of drugs from their binding sites (Coutinho, Luceck, Cheripho & Kuntzman, 1973;Odell, 1959;Olsen, Bennett & Porter, 1975;Williams, Blaschke, Meffin, Melman & Rowland, 1977), as can free fatty acids (Spector & Santor, 1973;Fredholme, Raine & Persson, 1975), and possibly other compounds known to be raised in the presence of jaundice (Williams et al, 1977;Perez-Mateo & Everill, 1977). The renal excretion of iotroxamide, like iodipamide and other cholangiographic agents, is likely to be predominantly by glomerular filtration (Fischer, 1965;Loeb, Berk, Feld & Wheeler, 1975).…”

Section: Biliary Excretion Studiesmentioning

confidence: 99%

Iotroxamide studies in man‐plasma binding, renal and biliary excretion studies in jaundiced and anicteric patients.

Doran¹,

Vennart²,

Cr³

et al. 1978

Brit J Clinical Pharma

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A New Approach to the Determination of Protein‐bound Bilirubin Displacement and Its Applications

Cited by 15 publications

References 18 publications

The effect of semisynthetic penicillins on the binding of bilirubin by neonatal serum.

The effect of semisynthetic penicillins on the binding of bilirubin by neonatal serum.

Bilirubin Adsorption In Vitro by Foetal and Adult Human Erythrocytes

Iotroxamide studies in man‐plasma binding, renal and biliary excretion studies in jaundiced and anicteric patients.

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