A new assay for interleukin-1 in the presence of interleukin-2

Falk, Werner; Krammer, Peter H.; Männel, Daniela N.

doi:10.1016/0022-1759(87)90030-5

Cited by 27 publications

(11 citation statements)

References 16 publications

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“…biological activity of interleukin 1 (IL-1) produced by monocytes; however, IL-1 -like activities of other cytokines and interleukin 2 also give a positive signal in the assay (22). Therefore, we have chosen the term LSA to describe the test.…”

Section: Resultsmentioning

confidence: 99%

Fish Oil–Enriched Diet and Reduction of Low-Dose Streptozocin–Induced Hyperglycemia: Inhibition of Macrophage Activation

et al. 1989

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Repeated low doses of streptozocin (STZ; 40 mg/kg, 5 injections/day) induce hyperglycemia in certain strains of mice after a latency of 1 wk. Omega-3 polyunsaturated fatty acids (omega 3FA) have been reported to suppress immune processes by blockade of the cyclooxygenase pathway of arachidonic acid metabolism. We investigated the effects of diets high in omega 3FA on the development of diabetes in the low-dose STZ-induced diabetes (LDSTZ-D) model. Male C57BL/6J mice were on a fish oil diet (FOD) as a source of omega 3FA 8 wk before STZ injection. Controls received laboratory chow only or a coconut oil diet (COD). Blood glucose levels in FOD mice were reduced (12.5 vs. 28 mM for COD mice, P less than .001) 60 days after STZ injection with a diet in which 20% of the calories were from fish oil. In FOD mice, immunohistology showed reduced numbers of class II antigen-expressing cells in pancreatic islets followed by a decreased extent of insulitis. FOD significantly decreased the number of Fc receptor-negative dendritic cells in cytospin preparations of islets isolated from diabetic mice. Interleukin 1-like activity of peritoneal exudate cell supernatants isolated from mice on FOD was reduced. FOD did not improve insulin secretion of isolated islets from LDSTZ-D mice. These data indicate a beneficial effect of FOD on the immune component of the mouse LDSTZ-D model.

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Section: Resultsmentioning

confidence: 99%

Fish Oil–Enriched Diet and Reduction of Low-Dose Streptozocin–Induced Hyperglycemia: Inhibition of Macrophage Activation

et al. 1989

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“…Proliferation reflects IL-1-induced expression of IL-2 receptors and the synthesis of the direct mitogen IL-2. However, the usefulness of the T-cell assay is limited because we now know it also directly measures IL-6 and TNFa (Gearing & Thorpe 1989, Uyttenhove et al 1988) and IL-1 response is augmented by IL-2 and IL-4 (Folk et al 1987). Although neutralizing antibodies can be used to discern the specificity of response to IL-I, they fail to account for unidentified cytokines.…”

Section: Il-1 Assaysmentioning

confidence: 99%

Antibodies and their Role in Interleukin‐1 Research

Gaffney

Anderson

Pfanenstiel

et al. 1991

Immunological Reviews

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The preceding paragraphs describe several uses of specific antibodies to IL-1. Standard radioimmunoassays and ELISA procedures were developed with demonstrated utility in studies attempting to quantitate the cytokine in cell culture fluids. In part, antibody-based assays circumvent the problems associated with bioassays. Bioassays are difficult to perform, often lack specificity due to the presence of competing cytokines and are insensitive when used with complex biological fluids due to non-specific protein binding and the presence of IL-1 antagonists. Thus, bioassays have not been broadly applicable with specimens in a clinical setting. In contrast, monoclonal antibodies and polyclonal antisera have provided a means of identifying functionally important regions of the IL-1 molecule, monitoring the post-translational processing of IL-1 which may include biologically inactive moieties, and developing new assays as illustrated by cell blot procedures.

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“…IL-1 activity was determined by the conventional costimulator assay [28], In brief, single cell suspension of C3H/ HeJ thymocytes (5 x 10s cells/culture) were cultured in the pres ence of phytohemagglutinin (PHA-M. 5 pg/ml. Sigma) and serial dilutions of the test samples for 3 days.…”

Section: Methodsmentioning

confidence: 99%

Spontaneous Monokine Release by Alveolar Macrophages in Chronic Sarcoidosis

Strausz

Männel

Pfeifer

et al. 1991

Int Arch Allergy Immunol

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In pulmonary sarcoidosis an activation of alveolar T lymphocytes and alveolar macrophages (AM) has been demonstrated. There is evidence that in contrast to acute disease a heightened T-cell response cannot be observed in the chronic phase of sarcoidosis. The role of AM in the inflammatory process of chronic sarcoidosis is not yet intensively evaluated. To address this question we measured the release of tumor necrosis factor alpha (TNFα) and interleukin-1 (IL-1) by AM of 39 patients with chronic sarcoidosis (duration > 4 years; 30 active, 9 inactive diseases) without therapy and correlated the monokine release with parameters of T-cell alveolitis and the course of the disease. The T4/T8 ratio was higher in the active than in the inactive group without reaching statistical significance. TNFα as well as IL-1 is spontaneously released by AM of the active group 2,099 ± 518 pg/ml TNFα/10⁶ cells/24 h and 8/13 (IL-l+/total) respectively. In the inactive group the AM release 375 ± 246 pg/ml TNFα/106 cells/24 h which is in the range of the control and 1 out of 5 patients was IL-1-positive. There was no correlation between the monokine release and any parameter of T-cell alveolitis. These data support the hypothesis that the inflammatory process in chronic sarcoidosis is dominated by the activity of AM and that this activity determines the course of the disease.

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A new assay for interleukin-1 in the presence of interleukin-2

Cited by 27 publications

References 16 publications

Fish Oil–Enriched Diet and Reduction of Low-Dose Streptozocin–Induced Hyperglycemia: Inhibition of Macrophage Activation

Fish Oil–Enriched Diet and Reduction of Low-Dose Streptozocin–Induced Hyperglycemia: Inhibition of Macrophage Activation

Antibodies and their Role in Interleukin‐1 Research

Spontaneous Monokine Release by Alveolar Macrophages in Chronic Sarcoidosis

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