A new biosensor based on the recognition of phages and the signal amplification of organic-inorganic hybrid nanoflowers for discriminating and quantitating live pathogenic bacteria in urine

Li, Yuxia; Xie, Guoming; Qiu, Juhui; Zhou, Dandan; Gou, Dan; Tao, Yiyi; Li, Yang; Chen, Hui

doi:10.1016/j.snb.2017.11.155

Cited by 77 publications

(36 citation statements)

References 38 publications

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“…In our previous study, we have reported an innovative strategy to significantly reduce the time taken to synthesize nanoflowers from the conventional 3 d to only 5 min via sonication treatment, which greatly facilitates their practical utilization . Based on these advantageous characteristics, nanoflowers have been utilized in various areas including biosensors, biocatalysts for protein digestion and dye removal, and biofuel cells…”

Section: Introductionmentioning

confidence: 99%

Magnetic Nanoparticles‐Embedded Enzyme‐Inorganic Hybrid Nanoflowers with Enhanced Peroxidase‐Like Activity and Substrate Channeling for Glucose Biosensing

Cheon

Adhikari

Chung

et al. 2019

Adv Healthcare Materials

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It is reported that glucose oxidase (GOx)‐copper hybrid nanoflowers embedded with Fe3O4 magnetic nanoparticles (MNPs) exhibit superior peroxidase‐mimicking activity as well as substrate channeling for glucose detection. This is due to the synergistic integration of GOx, crystalline copper phosphates and MNPs being in close proximity within the nanoflowers. The preparation of MNP‐embedded GOx‐copper hybrid nanoflowers (MNPs‐GOx NFs) begins with the facile conjugation of amine‐functionalized MNPs with GOx molecules via electrostatic attraction, followed by the addition of copper sulfate that leads to full blooming of the hybrid nanoflowers. In the presence of glucose, the catalytic action of GOx entrapped in the nanoflowers generates H2O2, which is subsequently used by peroxidase‐mimicking MNPs and copper phosphate crystals, located close to GOx molecules, to convert Amplex UltraRed substrate into a highly fluorescent product. Using this strategy, the target glucose is successfully determined with excellent selectivity, stability, and magnetic reusability. This biosensor based on hybrid nanoflowers also exhibits a high degree of precision and reproducibility when applied to real human blood samples. Such novel MNP‐embedded enzyme‐inorganic hybrid nanoflowers have a great potential to be expanded to any oxidases, which will be highly beneficial for the detection of various other clinically important target molecules.

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Section: Introductionmentioning

confidence: 99%

Magnetic Nanoparticles‐Embedded Enzyme‐Inorganic Hybrid Nanoflowers with Enhanced Peroxidase‐Like Activity and Substrate Channeling for Glucose Biosensing

Cheon

Adhikari

Chung

et al. 2019

Adv Healthcare Materials

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“…Recently, electric deposition based on the natural dipole properties of a phage has drawn more attention since it can induce the phages with proper orientation, increasing the accessibility of RBPs compared to random orientation. Attractive sensors have been developed by combining the high specific bacteriophages with low-cost and sensitive electrochemical detection techniques, the best detection limit of which has been down to even 1 cfu/mL [84]. Due to the environmental stability of phages under harsh conditions with proper storage, the developed sensors could also remain effective after a long period (over 3 months reported by Bhardwaj et al [52]).…”

Section: Discussionmentioning

confidence: 99%

“…In such an approach, phages are used either as a detection probe for target bacteria cells or as a tool for specific infection and further detection of the released cell content. Li et al proposed a promising biosensor by using the AMP magainin I as the capture probe and the phage-coated organic-inorganic hybrid nanoflowers (GOx&HRP-Cu 3 (PO 4 ) 2 ) as the detection probe [84]. The detection probes for E. coli cells were constructed by mixing the nanoflowers, gold nanoparticles (GNPs), thionine (Thi) and T4 bacteriophages, which can catalyze three cascade redox reactions in glucose working solution, serving as the signal amplification step.…”

Section: Phage Based Amperometric Sensorsmentioning

confidence: 99%

Phage-based Electrochemical Sensors: A Review

Chau

Lee

2019

Micromachines

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Phages based electrochemical sensors have received much attention due to their high specificity, sensitivity and simplicity. Phages or bacteriophages provide natural affinity to their host bacteria cells and can serve as the recognition element for electrochemical sensors. It can also act as a tool for bacteria infection and lysis followed by detection of the released cell contents, such as enzymes and ions. In addition, possible detection of the other desired targets, such as antibodies have been demonstrated with phage display techniques. In this paper, the recent development of phage-based electrochemical sensors has been reviewed in terms of the different immobilization protocols and electrochemical detection techniques.

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“…In electrochemical methods, the electric signal changes upon capturing of bacteria by virions deposited at the electrodes. There is an increasing number of published works utilizing the electrochemical approach to detect bacteria [26,[30][31][32][33][34][35][36]. Electrochemistry offers good sensitivity, low-cost analysis, and allows for miniaturization.…”

Section: Bacteriophages Deposited On Solid Substratesmentioning

confidence: 99%

Recent Progress in the Detection of Bacteria Using Bacteriophages: A Review

Paczesny

Richter

Hołyst

2020

Viruses

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Bacteria will likely become our most significant enemies of the 21st century, as we are approaching a post-antibiotic era. Bacteriophages, viruses that infect bacteria, allow us to fight infections caused by drug-resistant bacteria and create specific, cheap, and stable sensors for bacteria detection. Here, we summarize the recent developments in the field of phage-based methods for bacteria detection. We focus on works published after mid-2017. We underline the need for further advancements, especially related to lowering the detection (below 1 CFU/mL; CFU stands for colony forming units) and shortening the time of analysis (below one hour). From the application point of view, portable, cheap, and fast devices are needed, even at the expense of sensitivity.

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A new biosensor based on the recognition of phages and the signal amplification of organic-inorganic hybrid nanoflowers for discriminating and quantitating live pathogenic bacteria in urine

Cited by 77 publications

References 38 publications

Magnetic Nanoparticles‐Embedded Enzyme‐Inorganic Hybrid Nanoflowers with Enhanced Peroxidase‐Like Activity and Substrate Channeling for Glucose Biosensing

Magnetic Nanoparticles‐Embedded Enzyme‐Inorganic Hybrid Nanoflowers with Enhanced Peroxidase‐Like Activity and Substrate Channeling for Glucose Biosensing

Phage-based Electrochemical Sensors: A Review

Recent Progress in the Detection of Bacteria Using Bacteriophages: A Review

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