A new Borrelia infecting Lone Star ticks

Armstrong, PhilipM.; Rich, StephenM.; Smith, R. D.; Hartl, DanielL.; Spielman, Andrew; Telford, SamR.

doi:10.1016/s0140-6736(96)91604-9

Cited by 46 publications

(32 citation statements)

References 3 publications

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“…24 Borrelia lonestari was identified and characterized in A. americanum ticks in 1995 and was first isolated in 2004. 20,23,25 Borrelia lonestari has additionally been identified in A. americanum ticks that were removed from humans, including patients from Kentucky. 26 Ehrlichia chaffeensis is a gram-negative obligate intracellular bacterium and the etiologic agent of human monocytotrophic ehrlichiosis.…”

Section: Introductionmentioning

confidence: 99%

Infection Prevalences of Common Tick-borne Pathogens in Adult Lone Star Ticks (Amblyomma americanum) and American Dog Ticks (Dermacentor variabilis) in Kentucky

Fritzen

Huang²,

Westby³

et al. 2011

The American Society of Tropical Medicine and Hygiene

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Abstract. Rocky Mountain spotted fever, Lyme disease, and ehrlichiosis are tick-borne diseases that are reported annually in Kentucky. We conducted a survey to describe infection prevalence of tick-borne pathogens in Amblyomma americanum and Dermacentor variabilis ticks collected in Kentucky. During 2007-2008, we collected 287 ticks (179 D. variabilis and 108 A. americanum ) from canine, feral hog, horse, raccoon, white-tailed deer, and human hosts in six counties in Kentucky. Ticks were screened for Rickettsia spp., Borrelia spp., and Ehrlichia spp. by using polymerase chain reaction. Forty-one (14.3%) ticks (31 A. americanum and 10 D. variabilis ) were polymerase chain reaction-positive for a Rickettsia spp. Fourteen (4.9%) ticks (6 A. americanum and 8 D. variabilis ) were positive for E. chaffeensis , and 4 A. americanum (1.4%) were positive for E. ewingii . One (0.4%) A. americanum was positive for Borrelia lonestari .

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Section: Introductionmentioning

confidence: 99%

Infection Prevalences of Common Tick-borne Pathogens in Adult Lone Star Ticks (Amblyomma americanum) and American Dog Ticks (Dermacentor variabilis) in Kentucky

Fritzen

Huang²,

Westby³

et al. 2011

The American Society of Tropical Medicine and Hygiene

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“…Since the mid-1980s, physicians have described a Lyme disease-like illness in patients from the southeastern and southcentral United States in which an erythema migrans rash and mild flu-like symptoms develop following the bite of a lone star tick, Amblyomma americanum (2,3,12,19,31,43). This disease is alternatively referred to as southern tick-associated rash illness (STARI), Master's disease, or southern Lyme disease (23,30).…”

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confidence: 99%

“…has been reported from lone star ticks, from white-tailed deer, and from the skin of a patient with STARI, as well as from a lone star tick removed from that patient (2,10,11,23,32,47). The organism, tentatively named Borrelia lonestari, has been described only by PCR amplification of the flagellin B gene and 16S ribosomal DNA (rDNA).…”

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confidence: 99%

First Culture Isolation of Borrelia lonestari , Putative Agent of Southern Tick-Associated Rash Illness

Varela¹,

Luttrell

Howerth³

et al. 2004

J Clin Microbiol

108

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Southern tick-associated rash illness (STARI) is a Lyme disease-like infection described in patients in the southeastern and south-central United States, where classic Lyme disease is relatively rare. STARI develops following the bite of a lone star tick (Amblyomma americanum) and is thought to be caused by infection with an "uncultivable" spirochete tentatively named Borrelia lonestari. In this study, wild lone star ticks collected from an area where B. lonestari is endemic were cocultured in an established embryonic tick cell line (ISE6). The cultures were examined by dark-field microscopy for evidence of infection, and spirochete identity and morphology were evaluated by flagellin B and 16S rRNA gene sequence, by reaction to Borrelia-wide and B. burgdorferi-specific monoclonal antibodies, and by electron microscopy. Live spirochetes were first visualized in primary culture of A. americanum ticks by dark-field microscopy 14 days after the cell culture was inoculated. The sequences of the flagellin B and 16S rRNA genes of cultured spirochetes were consistent with previously reported sequences of B. lonestari. The cultured spirochetes reacted with a Borrelia-wide flagellin antibody, but did not react with an OspA antibody specific to B. burgdorferi, by indirect fluorescent antibody testing. Electron microscopy demonstrated organisms that were free and associated with ISE6 cells, with characteristic Borrelia sp. morphology. This study describes the first successful isolation of B. lonestari in culture, providing a much needed source of organisms for the development of diagnostic assays and forming a basis for future studies investigating the role of the organism as a human disease agent.

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“…However, a Lyme disease-like illness that develops following the bite of the Lone Star tick, Amblyomma americanum, has been described (2,7,12,17,27,28). Individuals affected with this illness, termed "southern tick-associated rash illness" (STARI), commonly develop a localized expanding circular skin rash (erythema migrans) at the site of the tick bite similar to that seen with classic Lyme disease.…”

mentioning

confidence: 99%

Detection of Borrelia lonestari , Putative Agent of Southern Tick-Associated Rash Illness, in White-Tailed Deer ( Odocoileus virginianus ) from the Southeastern United States

Moore¹,

Varela²,

Yabsley

et al. 2003

J Clin Microbiol

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A new Borrelia infecting Lone Star ticks

Cited by 46 publications

References 3 publications

Infection Prevalences of Common Tick-borne Pathogens in Adult Lone Star Ticks (Amblyomma americanum) and American Dog Ticks (Dermacentor variabilis) in Kentucky

Infection Prevalences of Common Tick-borne Pathogens in Adult Lone Star Ticks (Amblyomma americanum) and American Dog Ticks (Dermacentor variabilis) in Kentucky

First Culture Isolation of Borrelia lonestari , Putative Agent of Southern Tick-Associated Rash Illness

Detection of Borrelia lonestari , Putative Agent of Southern Tick-Associated Rash Illness, in White-Tailed Deer ( Odocoileus virginianus ) from the Southeastern United States

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