A new candidate for the regulation of erythropoiesis

Kurtz, Armin; Jelkmann, Wolfgang; Bauer, Christian

doi:10.1016/0014-5793(82)81081-8

Cited by 93 publications

(37 citation statements)

References 20 publications

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“…This observation is in apparent discordance with previously published data, 5,8,[17][18][19][26][27][28][29][30][31] and could be partially explained by the fact that in our study we have been cloning bone marrow CD34 + cells, depleted from hematopoietic accessory cells. These latter cells could secrete KL or IL-3 and/or other factors which can co-stimulate growth of the BFU-E colonies.…”

Section: Discussioncontrasting

confidence: 99%

“…Several reports suggested that INS and/or IGF-I are required to costimulate with EpO erythroid colony formation in serum-free cloning medium. 5,8,[17][18][19][26][27][28][29][30][31] Nevertheless, we were not able to reproduce this observation when CD34…”

Section: Erythroid Colony Formation In Serum-free Inssupplemented Cumentioning

confidence: 99%

“…We also studied the development of BFU-E colonies compared to the effect of other cytokines and growth factors which have been reported to enhance erythropoiesis. 4,6,[16][17][18][19] Finally, we analyzed by FACS and RT-PCR the expression of INS-R and IGF-IR in the early progenitor cells and their more differentiated, cultured derivatives. Our results suggest that neither INS nor IGF-I are critical for human erythropoiesis.…”

Section: Introductionmentioning

confidence: 99%

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The role of insulin (INS) and insulin-like growth factor-I (IGF-I) in regulating human erythropoiesis. Studies in vitro under serum-free conditions – comparison to other cytokines and growth factors

et al. 1998

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The role of insulin (INS), and insulin-like growth factor-I (IGF-I Both INS and IGF-I activated signal transduction in maturing human erythropoietic cells as determined by phosphorylation of the insulin receptor substrate-2 (IRS-2) protein. We also found by RT-PCR that mRNA coding for INS-R is expressed in FACS sorted CD34؉ , c-kit-R ؉ marrow cells, and in cells isolated from BFU-E and CFU-GM colonies. Expression of INS-R protein on these cells was subsequently confirmed by cytofluorometry. In contrast, the receptor for insulin-like growth factor-I (IGF-IR) was not detected on CD34؉ cells, and was first easily detectable on more differentiated cells derived from day 6 BFU-E and CFU-GM colonies. We conclude that INS and IGF-I may be survival factors for human CD34 ؉ cells, but are not required during early erythropoiesis. In contrast, both growth factors may play some role at the final stages of erythroid maturation.

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Section: Discussioncontrasting

confidence: 99%

Section: Erythroid Colony Formation In Serum-free Inssupplemented Cumentioning

confidence: 99%

Section: Introductionmentioning

confidence: 99%

See 1 more Smart Citation

The role of insulin (INS) and insulin-like growth factor-I (IGF-I) in regulating human erythropoiesis. Studies in vitro under serum-free conditions – comparison to other cytokines and growth factors

et al. 1998

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“…Cell culture assays for Ep are also sensitive to such factors (1, [8][9][10][11][12][13]. The observed effect varies with the culture system used, indicating that these factors may influence erythropoiesis in different ways and at various stages of differentiation (8,14).…”

Section: Discussionmentioning

confidence: 99%

Plasma Erythropoiesis Stimulating Factor(s) in Neonatal Mice: In Vitro Dose Response and Chromatography Studies

Sanengen

Halvorsen

1987

Pediatr Res

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ABSTRACT. High levels of plasma erythropoiesis stimulating factor(s) (ESF) have been found in neonatal WLOmice during rapid growth. A previous study on hypertransfused neonatal animals indicated that the high ESF could not be due to the concomitant postnatal anemia alone. The present investigation was performed to answer the question: Is the high plasma ESF in neonatal WLO-mice erythropoietin (Ep) alone, or Ep in combination with other factors? The ESF of plasma from 20-day-old animals and standard Ep were compared in a cell culture assay for ESF based on erythroid colony formation, and also by means of gel filtration chromatography and affinity chromatography. Nonfractionated plasma and standard Ep showed parallel dose response curves and additive activity in the ESF assay. After gel filtration the detectable ESF of plasma was eluted in the same position as that of standard Ep, corresponding to an estimated molecular weight range of 34-65000 daltons. The ESF of intact plasma, fractionated plasma, and standard Ep were identically bound to and eluted from the affinity chromatography column. These results show that the ESF of plasma from 20-day-old animals can neither be separated into several factors, nor distinguished from that of standard Ep by the methods used. It is therefore concluded that the high plasma ESF found in neonatal WLOmice probably consists of Ep alone. (Pediatr Res 21: 148-151,1987) Abbreviations CFU,,:, erythroid colony forming cell Ep, erythropoietin ESF, erythropoiesis stimulating factor(s) MW, molecular weight PBS, phosphate-buffered saline S-200 column, Sephacryl S-200 superfine column WG-column, wheat germ lectin-Sepharose 6MB column V,, void volume IGF-I, insulin-like growth factor I Erythropoiesis in the adult mammal is regulated mainly through Ep in response to tissue hypoxia, although non-Ep factors may be involved (1). Erythroid progenitor cells in culture are also sensitive to these factors. Thus, cell culture assays for Ep based on erythroid colony formation determine ESF, which is the net activity of Ep and other erythropoietic stimulatory factors and possible inhibitory factors present in the samples.

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“…In vitro, it stimulates the proliferation of a wide variety of cultured cells that are primarily of mesodermal origin (3)(4)(5). In addition, it can cause some cells to differentiate including chondrocytes (6), myoblasts (7), osteoblasts (8) and erythroid precursors (9). In vivo, IGF-I regulates postnatal skeletal growth (5,10).…”

Section: Introductionmentioning

confidence: 99%

Sequences of liver cDNAs encoding two different mouse insulin-like growth factor I precursors

Bell¹,

Stempien²,

Fong³

et al. 1986

Nucl Acids Res

214

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Complementary DNAs encoding mouse liver insulin-like growth factor I (ICF-I) have been isolated and sequenced. Alternative RNA splicing results in the synthesis of two types of mouse IGF-I precursor that differ in the size and sequence of the COOH-terminal peptide.The sequences of the signal peptides, IGF-I moieties and the first 16 amino acids of the COOH-terminal peptides or E-domains of the two precursors are identical.The sequence difference results from the presence in preproIGF-IB mRNA of a 52 base insertion which introduces a 17 amino acid segment into the COOH-terminal peptide of preproIGF-IB and also causes a shift in the reading frame of the mRNA. As a consequence of this insertion, the COOH-terminal 19 and 25 amino acids of mouse preproIGF-IA and -IB, respectively, are different. The sequences of mouse and human preproIGF-IA are highly conserved and possess 94% identity.In contrast, the sequences of mouse and human preproIGF-IB are quite different in the region of the COOH-terminal peptide. A comparison of the sequences of mouse and human preproIGF-IB mRNA indicates that they are generated by different molecular mechanisms.

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A new candidate for the regulation of erythropoiesis

Cited by 93 publications

References 20 publications

The role of insulin (INS) and insulin-like growth factor-I (IGF-I) in regulating human erythropoiesis. Studies in vitro under serum-free conditions – comparison to other cytokines and growth factors

The role of insulin (INS) and insulin-like growth factor-I (IGF-I) in regulating human erythropoiesis. Studies in vitro under serum-free conditions – comparison to other cytokines and growth factors

Plasma Erythropoiesis Stimulating Factor(s) in Neonatal Mice: In Vitro Dose Response and Chromatography Studies

Sequences of liver cDNAs encoding two different mouse insulin-like growth factor I precursors

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