A New Chemical Approach to the Efficient Generation of Mouse Embryonic Stem Cells

Baharvand, Hossein; Hassani, Seyedeh‐Nafiseh

doi:10.1007/978-1-62703-348-0_2

Cited by 9 publications

(2 citation statements)

References 19 publications

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“…Mouse embryonic derivative stem cell line, RoyanB20, was used in this study and retained in an undifferentiated state as described (Baharvand and Hassani 2013). Cells were cultured in serum free medium, a mixed cocktail of Dulbecco's modified Eagle medium/Ham's-Nutrient Mixture F-12 (DMEM F12) with Neurobasal media (Gibco, USA) supplemented with 0.5% BSA, 2 mM glutamine, 0.1 mM nonessential amino acids, 1% penicillin-streptomycin (all from Invitrogen, USA), 0.1 mM β-mercaptoethanol (Sigma-Aldrich, USA) and 1000 U/mL leukemia inhibitory factor (LIF; Chemicon, USA), 1 μM PD0325901(Sigma, USA) and 10 μM SB431542 (Sigma, USA),1% N2 (Gibco, USA) , 2% B27 (Gibco, USA).…”

Section: Mescs Culture and Neural Differentiation Stagesmentioning

confidence: 99%

Zinc finger protein 521 overexpression increased transcript levels of Fndc5 in mouse embryonic stem cells

et al. 2016

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Zinc finger protein 521 is highly expressed in brain, neural stem cells and early progenitors of the human hematopoietic cells. Zfp521 triggers the cascade of neurogenesis in mouse embryonic stem cells through inducing expression of the early neuroectodermal genes Sox1, Sox3 and Pax6. Fndc5, a precursor of Irisin has inducing effects on the expression level of brain derived neurotrophic factor in hippocampus. Therefore, it is most likely that Fndc5 may play an important role in neural differentiation. To exhibit whether the expression of this protein is under regulation with Zfp521, we overexpressed Zfp521 in a stable transformants of mESCs expressing EGFP under control of Fndc5 promoter. Increased expression of Zfp521 enhanced transcription levels of both EGFP and endogenous Fndc5. This result was confirmed by overexpression the aforementioned vectors in HEK cells and indicated that Zfp521 functions upstream of Fndc5 expression. It is most likely that Zfp521 may act through the binding to its response element on Fndc5 core promoter. Therefore it is concluding that an enhanced expression of Fndc5 in neural progenitor cells is stimulated by Zfp521 overexpression in these cells.

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Section: Mescs Culture and Neural Differentiation Stagesmentioning

confidence: 99%

Zinc finger protein 521 overexpression increased transcript levels of Fndc5 in mouse embryonic stem cells

et al. 2016

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“…The above studies exploring the use of small molecule compounds on iPSC provided theoretical and technical basis to achieve exogenous gene free livestock naive iPSC. [62]. The recent studies [2] have showed that morphological and biological characteristics of human ES was more similar to the mouse primed epiblast stem cells [counterpart to primed state].…”

Section: Small Molecule Compounds Replaced Transcription Factors For mentioning

confidence: 99%

An Insight on Small Molecule Induced Foot-Print Free Naive Pluripotent Stem Cells in Livestock

Li¹,

Li²,

Zhang³

et al. 2015

SCD

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Bona fide embryonic stem cell (ESC) lines from livestock species have been challenging to derive and maintain, contrasting mouse and human ESCs. However, induced pluripotent stem cells (iPSC) generated by reprogramming somatic cells tender an option, as they display characteristic features of ESC. The comprehension that induced pluripotent stem cells (iPSC) could be created with in no time also holds the potential of allowing pluripotent cells to be derived from animal models vital in biomedical research. Endeavors to produce bona fide pluripotent stem cells (PSC) from livestock have been going on for more than two decades. But, attempts to derive bona fide livestock iPS cells have met with limited success. Recently it's been reported that small molecules can augment reprogramming efficiency and may be used to substitute few or all transcription factors used for reprogramming. It is assumed that the reprogramming factors are conserved among species, and this small molecule reprogramming approach will probably apply to livestock species as well. So this review will focus mainly on the accomplishments of small molecules on accelerating cell reprogramming and obtaining naive pluripotency, and raise a new insight on, exogenous genes free, livestock naive iPSC generation with a new bullet, small molecule.

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Inhibition of TGFβ Signaling Promotes Ground State Pluripotency

Hassani

Totonchi

Sharifi-Zarchi

et al. 2013

Stem Cell Rev and Rep

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Embryonic stem (ES) cells are considered to exist in a ground state if shielded from differentiation triggers. Here we show that FGF4 and TGFβ signaling pathway inhibitors, designated R2i, not only provide the ground state pluripotency in production and maintenance of naïve ES cells from blastocysts of different mouse strains, but also maintain ES cells with higher genomic integrity following long-term cultivation compared with the chemical inhibition of the FGF4 and GSK3 pathways, known as 2i. Global transcriptome analysis of the ES cells highlights augmented BMP4 signaling pathway. The crucial role of the BMP4 pathway in maintaining the R2i ground state pluripotency is demonstrated by BMP4 receptor suppression, resulting in differentiation and cell death. In conclusion, by inhibiting TGFβ and FGF signaling pathways, we introduce a novel defined approach to efficiently establish the ground state pluripotency.

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A New Chemical Approach to the Efficient Generation of Mouse Embryonic Stem Cells

Cited by 9 publications

References 19 publications

Zinc finger protein 521 overexpression increased transcript levels of Fndc5 in mouse embryonic stem cells

Zinc finger protein 521 overexpression increased transcript levels of Fndc5 in mouse embryonic stem cells

An Insight on Small Molecule Induced Foot-Print Free Naive Pluripotent Stem Cells in Livestock

Inhibition of TGFβ Signaling Promotes Ground State Pluripotency

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