A New Chimeric Drug Delivery Nano System (chi-aDDnS) Composed of PAMAM G 3.5 Dendrimer and Liposomes as Doxorubicin's Carrier. <I>In Vitro</I> Pharmacological Studies

Abstract

Chimeric advanced Drug Delivery nano Systems (chi-aDDnSs) could be defined as mixed nanosystems due to the combination process of nanobiomaterials and can offer advantages as drug carriers. The role of the release modulator from the liposomal system is undertaken by the dendrimer molecules leading to new pharmacokinetic and, probably, pharmacological properties of the chimeric system. In this work, a conventional DOPC/DPPG liposomal system and a new chi-aDDnS composed of liposomes (DOPC/DPPG) incorporating PAMAM G3,5 has been developed, Doxorubicin (Dox) was loaded in the systems and the final formulations were lyophilized. The physicochemical (spectroscopic and calorimetric) investigation concerning the chi-aDDnS, revealed a strong interaction between both lipophilic and hydrophilic parts of the liposomal membrane and the dendrimer, with the induction of multiple energetic states. These states are probably the basis of higher Dox encapsulation and slower release rate compared to the respective conventional liposome. These results, in conjunction with the increase in TI observed in two investigated cancer cell lines (i.e., MB231 and MCF7), compared to the respective conventional liposomal system and to the free Dox, make this new chi-aDDnS the basic candidate for further in vivo investigations.

Alpha‐Tocopheryl Succinate‐Conjugated G5 PAMAM Dendrimer Enables Effective Inhibition of Ulcerative Colitis

“…Dendrimers, as a class of well‐defined and highly branched synthetic macromolecules that have specific shape, size and chemical functionality, are potential carriers to deliver drugs, genes, and diagnostic agents via various administration routes including gavage, intravenous injection, and intramuscular injection, and the mechanisms of drug release and their biodistribution are various . Generation 5 (G5) PAMAM dendrimers among all generations exhibit the uniform spherical shape with appropriate size and number of terminal groups, showing unique advantages in drug delivery, including targeting capability, rapid cell entry, and easily cross biological barriers .…”

Anticancer siRNA cocktails as a novel tool to treat cancer cells. Part (A). Mechanisms of interaction

“…Those now being most used are complexes of nucleic acids with liposomes (lipoplexes) and cationic linear polymers (polyplexes). However, the best perspective for cancer gene therapy seems to be nanomaterials (Gardikis et al, 2011;Shcharbin et al, 2014), and foremost are dendrimers. These are synthetic polymers with a diameter of 3-10 nm.…”

Polymersomes from Polypeptide Containing Triblock Co- and Terpolymers for Drug Delivery against Pancreatic Cancer: Asymmetry of the External Hydrophilic Blocks

“…Aiming to address these issues, drug carriers (Drug Delivery Systems, DSS) have been developed with the aid of nanotechnology. DDS have been proven to be of high value as they maximize the therapeutic benefits by delivering drugs, genes and proteins with enhanced therapeutic efficacy, reduced dose, low dosing frequency and fewer side effects …”