A new class of phytoalexins from grapevines

Langcake, P.; Pryce, Robert J.

doi:10.1007/bf02124034

Cited by 461 publications

(338 citation statements)

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“…Resveratrol (3,4 0 ,5-trihydroxy-trans-stilbene), a polyphenolic phytoalexin found in the skin of red grapes and a variety of other fruits, functions in plants to protect against fungal infections (5). A variety of studies have reported that resveratrol has cardioprotective, antioxidant, anti-inflammatory, antibacterial, and antiviral activities (6).…”

Section: Introductionmentioning

confidence: 99%

Resveratrol Inhibits Proliferation and Survival of Epstein Barr Virus–Infected Burkitt's Lymphoma Cells Depending on Viral Latency Program

Leo

Arena

Stecca

et al. 2011

Molecular Cancer Research

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Resveratrol (3,4 0 ,5-trihydroxy-trans-stilbene), a polyphenolic natural product, shows chemopreventive properties against several cancers, heart diseases, inflammation, and viral infections. Epstein Barr virus (EBV), a g-herpesvirus, contributes to the development of several human cancers including Burkitt's lymphoma (BL). In this study, we asked whether treatment with resveratrol would affect the viability of EBV-positive BL cells displaying different forms of latency. We report here that resveratrol, regardless of EBV status, induces caspasedependent apoptosis by arresting cell-cycle progression in G 1 phase. However, resveratrol strongly induced apoptosis in EBV(À) and latency I EBV(þ) cells, whereas latency II and latency III EBV(þ) BL cells showed a survival advantage that increased with the extent of the pattern of viral gene expression. Resveratrol-induced cellcycle arrest and apoptosis occurred in association with induction of p38 MAPK phosphorylation and suppression of ERK1/2 signaling pathway. Moreover, NF-kB DNA-binding activity was inhibited in all BL lines except EBV (þ) latency III cells.LMP1 oncogene, which is expressed in latency III phenotype, is involved with the higher resistance to the antiproliferative effect of resveratrol because siRNA-mediated inhibition of LMP1 greatly increased the sensitivity of latency III BL cells as well as that of lymphoblastoid cell lines to the polyphenol. We propose that a combined resveratrol/siRNA strategy may be a novel approach for the treatment of EBV-associated B-cell malignancies in which the viral pattern of gene expression has been defined. Mol Cancer Res; 9(10); 1346-55. Ó2011 AACR.

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Section: Introductionmentioning

confidence: 99%

Resveratrol Inhibits Proliferation and Survival of Epstein Barr Virus–Infected Burkitt's Lymphoma Cells Depending on Viral Latency Program

Leo

Arena

Stecca

et al. 2011

Molecular Cancer Research

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“…Together with these alterations in cell physiology and metabolism, elicitation induced the synthesis and accumulation of resveratrol (Figs 7-9), a potent phytoalexin of grapevines (Langcake & Pryce, 1977). Resveratrol is an inhibitor of both the germination of conidia and mycelial growth of B. cinerea (Hoos & Blaich, 1990).…”

Section: Discussionmentioning

confidence: 99%

“…Screening TLC plates for resveratrol, pterostilbene and e-viniferin in several TLC systems (Hillis & Ishikura, 1968;Pool et al,, 1981) showed that the only compound present in detectable amounts was resveratrol. Pterostilbene was detected in neither case, while e-viniferin, the product of oxidative coupling of resveratrol (Langcake & Pryce, 1977), was only detectable in trace amounts, which hampered further characterization and quantification.…”

Section: Resveratrolmentioning

confidence: 93%

“…On the other hand, peak 7 (Fig. 5 b) co-eluted with an ion fragment of m/z = 444 (relative abundance 5%), which may have been the molecular ion corresponding to the TMS-derivative of resveratrol (Jeandet et al, 1991), a potent phytoalexin of grapevines (Langcake & Pryce, 1977). This ion fragment was not detected in autoclaved elicitor controls.…”

Section: Resveratrolmentioning

confidence: 95%

“…For this standard, resveratrol concentration was calculated from the absorbance at 306 nm using e.,,,^ = 26800 M-^cm"^ (Langcake & Pryce, 1977). Retention volume {R^) for resveratrol under these conditions (/?,.…”

Section: Identification and Quantification Of Resveratrolmentioning

confidence: 99%

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Resveratrol production as a part of the hypersensitive‐like response of grapevine cells to an elicitor from Trichoderma viride

et al. 1993

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SUMMARYSuspension cell cultures of grapevine {Vitis vinifera, cv. Monastrell) treated with an elicitor (cellulase, Onozuka R-10) from Trichoderma viride showed a hypersensitive-like response. This was characterized by cell plasmolysis and was accompanied by localized cell death, which was concomitant with cell culture browning, itself probably due to an activation of oxidative phenolic metabolism driven by a large increase in endogenous levels of H.^O2. In addition to these responses, the treatment of cell cultures with the elicitor produced an increase in amounts of benzoic acid and of resveratrol, the latter a potent phytoalexin of grapevines. This hypersensitive-like response was specific since none of the above responses was obtained with other cell wall-degrading enzymes from several sources, or with inocula of either mycelial extracts or culture filtrates of Botrytis cinerea. These results are discussed in the light of a disease-resistance reaction induced in grapevine cells by a product of T. viride, a fungal agent characterized by its effective biocontrol of Botrytis cinerea, the causal agent of grey mould in grapevines.

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