A new Clethrionomys-derived hantavirus from Germany: evidence for distinct genetic sublineages of Puumala viruses in Western Europe

Heiske, Andreas; Anheier, Bärbel; Pilaski, J.; Volchkov, Viktor E.; Feldmann, Heinz

doi:10.1016/s0168-1702(99)00024-6

Cited by 23 publications

(21 citation statements)

References 37 publications

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“…The earlier inferred PUUV phylogenies (1,3,13,19,24,43,45,46,48,49) were obtained using DM methods. Since they are robust they are widely used, but they should be applied with careful validation.…”

Section: Discussionmentioning

confidence: 99%

“…Nevertheless, if the master genotype and phenotype have high fitness to the environment, the quasispecies populations might be stable for a long period of time. This is most probably the case for PUUV and other hantaviruses, which have been well adapted to their natural hosts since long ago.Previous publications on PUUV evolution (1,3,13,19,24,43,45,46,48,49) have focused mainly on specific geographical areas, including only a limited number of sequences, and all the phylogenetic analyses have been performed with distant matrix methods. Recently, a substantial set of new complete PUUV S segment sequences became available (references 3 and 13 and our unpublished results).…”

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confidence: 99%

“…1): Finland (45,46,48,62), Sweden (24,25,38), Norway (38), Denmark (3), Russia (3,16,48,49,66), Belgium (6,13), Austria (1,5), and Germany (19,43). So far, over 100 partial or complete PUUV sequences have been deposited in GenBank.…”

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confidence: 99%

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Molecular Evolution of Puumala Hantavirus

Sironen

Vaheri

Plyusnin

2001

J Virol

114

112

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Puumala virus (PUUV) is a negative-stranded RNA virus in the genus Hantavirus, family Bunyaviridae. In this study, detailed phylogenetic analysis was performed on 42 complete S segment sequences of PUUV originated from several European countries, Russia, and Japan, the largest set available thus far for hantaviruses. The results show that PUUV sequences form seven distinct and well-supported genetic lineages; within these lineages, geographical clustering of genetic variants is observed. The overall phylogeny of PUUV is star-like, suggesting an early split of genetic lineages. The individual PUUV lineages appear to be independent, with the only exception to this being the Finnish and the Russian lineages that are closely connected to each other. Two strains of PUUV-like virus from Japan form the most ancestral lineage diverging from PUUV. Recombination points within the S segment were searched for and evidence for intralineage recombination events was seen in the Finnish, Russian, Danish, and Belgian lineages of PUUV. Molecular clock analysis showed that PUUV is a stable virus, evolving slowly at a rate of 0.7 ؋ 10 ؊7 to 2.2 ؋ 10 ؊6 nt substitutions per site per year.Puumala virus (PUUV) belongs to the genus Hantavirus of the family Bunyaviridae (12). Like other members of this family, PUUV is an enveloped virus with a segmented, singlestranded RNA genome of negative polarity. The large (L) segment of 6.5 kb encodes the viral RNA polymerase, the 3.7-kb medium (M) segment encodes the two surface glycoproteins, and the 1.8-kb small (S) segment encodes the nucleocapsid protein (N).The natural host of PUUV is the bank vole, Clethrionomys glareolus, which belongs to the Arvicolinae subfamily of the Muridae family. The bank vole is found in most of Europe, excluding the Mediterranean coast and the northernmost areas ( Fig. 1) (40). The virus causes a life-long persistent and asymptomatic infection in rodents (47). In contrast, in humans PUUV is pathogenic, causing nephropathia epidemica, a mild form of hemorrhagic fever with renal syndrome (HFRS) (8).PUUV from the following countries has been genetically characterized ( Fig. 1): Finland (45,46,48,62), Sweden (24,25,38), Norway (38), Denmark (3), Russia (3,16,48,49,66), Belgium (6, 13), Austria (1, 5), and Germany (19, 43). So far, over 100 partial or complete PUUV sequences have been deposited in GenBank. These include 38 complete S segment sequences, 9 complete M segment sequences, and 2 complete L segment sequences. In general, the phylogenetic relationships between different hantaviruses and their rodent hosts mirror each other, supporting the idea of coevolution of the virus and its host (44). Furthermore, different strains of a given hantavirus type, including PUUV, show geographical clustering (48, 49) reflecting the sometime complicated history of host range movements. The most recent bank vole range changes are due to the recolonization of Europe and especially Fennoscandia after the last ice age 10,000 years ago (29). Earlier it has been shown that these migr...

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Section: Discussionmentioning

confidence: 99%

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confidence: 99%

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Molecular Evolution of Puumala Hantavirus

Sironen

Vaheri

Plyusnin

2001

J Virol

114

112

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“…The belief in long-standing coevolutionary relationships between hantaviruses and their reservoir rodent host species is supported by virus and rodent gene phylogenies. That is, phylogenetic analyses, based on full-length viral genomic sequences and rodent mitochondrial DNA (mtDNA) or nuclear gene sequences, indicate that antigenically distinct hantaviruses segregate into clades, which parallel the evolution of rodents in the subfamilies Murinae, Arvicolinae, Neotominae, and Sigmodontinae (23,25,26,28,39,54). Previously, this phylogenetic insight has been successfully employed to direct the discovery of new hantaviruses, such as those found in the Korean field mouse (Apodemus peninsulae) (5) and the royal vole (Myodes regulus) (47).…”

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confidence: 99%

Characterization of Imjin Virus, a Newly Isolated Hantavirus from the Ussuri White-Toothed Shrew ( Crocidura lasiura )

Song

Kang

et al. 2009

J Virol

102

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Until recently, the single known exception to the rodent-hantavirus association was Thottapalayam virus (TPMV), a long-unclassified virus isolated from the Asian house shrew (Suncus murinus)Hantaviruses (family Bunyaviridae, genus Hantavirus) are medically important rodent-borne pathogens, causing hemorrhagic fever with renal syndrome (HFRS) and hantavirus cardiopulmonary syndrome (HCPS). The belief in long-standing coevolutionary relationships between hantaviruses and their reservoir rodent host species is supported by virus and rodent gene phylogenies. That is, phylogenetic analyses, based on full-length viral genomic sequences and rodent mitochondrial DNA (mtDNA) or nuclear gene sequences, indicate that antigenically distinct hantaviruses segregate into clades, which parallel the evolution of rodents in the subfamilies Murinae, Arvicolinae, Neotominae, and Sigmodontinae (23,25,26,28,39,54). Previously, this phylogenetic insight has been successfully employed to direct the discovery of new hantaviruses, such as those found in the Korean field mouse (Apodemus peninsulae) (5) and the royal vole (Myodes regulus) (47).Renewed interest in the role of nonrodent reservoirs in the evolution of hantaviruses has been spurred by recent analysis of the entire genome of Thottapalayam virus (TPMV), a hantavirus isolated from the Asian house shrew (Suncus murinus) (10, 61), which revealed a separate phylogenetic clade, suggesting early evolutionary divergence from rodent-borne hantaviruses (44, 56). Armed with oligonucleotide primers designed on the basis of conserved regions of the TPMV genome and guided by long-ignored reports of serologic and antigenic evidence of hantavirus infection in shrews (20, 33, 52), we have previously detected genetically distinct hantaviruses in the Eurasian common shrew (Sorex araneus) from Switzerland (45); the Chinese mole shrew (Anourosorex squamipes) from Vietnam (46); and the northern short-tailed shrew (Blarina brevicauda), masked shrew (Sorex cinereus), and dusky shrew (Sorex monticolus) from the United States (1, 2) by reverse transcription-PCR (RT-PCR). Novel hantavirus genomes in Therese's shrew (Crocidura theresae) from Guinea (29); the vagrant shrew (Sorex vagrans), Trowbridge's shrew (Sorex trowbridgii), and the American water shrew (Sorex palustris) from the United States (H. J. Kang and R. Yanagihara, unpublished data); and the flat-skulled shrew (Sorex roboratus) and Laxmann's shrew (Sorex caecutiens) from Russia (Kang and Yanagihara, unpublished) have also been detected.Here, we report the antigenic, genetic, and phylogenetic characterization of a newly identified hantavirus, designated Imjin virus (MJNV), isolated from Ussuri white-toothed shrews of the species Crocidura lasiura (order Soricomorpha, family Soricidae, subfamily Crocidurinae) captured near the demilitarized zone (DMZ) in the Republic of Korea. The discovery of MJNV and other soricid-borne hantaviruses from widely separated geographic regions, spanning four continents,

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“…In Germany, the detection of PUUV in a vole from the North-Western part of the country was reported in 1999 (Heiske et al, 1999). From various M. glareolus specimens sampled during or after a PUUV outbreak in the Bavarian Forest (South-East Germany) in 2004, molecular phylogenetic analyses have been carried out (Essbauer et al, 2006;Schilling et al, 2007;Mertens et al, 2011).…”

Section: Puumala Virusmentioning

confidence: 99%

The broad spectrum of hantaviruses and their hosts in Central Europe

Klempa¹,

Radosa²,

Dh³

2013

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Summary. -Hantaviruses are considered to be emerging viruses due to their increasing significance as human pathogens and their cyclic reappearance during outbreaks. Central Europe is an important endemic region for hantavirus infections. Reflecting the presence of all relevant small mammals serving as reservoir hosts, close to all recognized European hantaviruses occur also in Central Europe. Important human pathogens, Puumala and Dobrava-Belgrade viruses, are present and cause hemorrhagic fever with renal syndrome of various severities. Moreover, several of the newly recognized shrew-and mole-borne hantaviruses are present. In this review, we summarize current data on molecular detection of hantaviruses in reservoir hosts as well as on molecular epidemiology of human hantavirus infections in Central Europe.

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A new Clethrionomys-derived hantavirus from Germany: evidence for distinct genetic sublineages of Puumala viruses in Western Europe

Cited by 23 publications

References 37 publications

Molecular Evolution of Puumala Hantavirus

Molecular Evolution of Puumala Hantavirus

Characterization of Imjin Virus, a Newly Isolated Hantavirus from the Ussuri White-Toothed Shrew ( Crocidura lasiura )

The broad spectrum of hantaviruses and their hosts in Central Europe

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