“…The various procedures used for drug level measurements were reviewed (1). It is one of the few drugs for which plasma concentrations are routinely monitored, and many GLC methods (2)(3)(4)(5)(6)(7)(8)(9)(10)(11)(12)(13)(14) are suitable for the assay of steady-state levels. The dose-dependent biotransformation of phenytoin and variations in its metabolism in treated subjects require that assay methods for determining plasma concentrations of the unchanged drug and its principal metabolite, 541-hydroxyphenyl)-5-phenylhydantoin (15), suitable for single-dose pharmacokinetic studies be developed.…”