A new continuous cell line from Blaps rhynchoptera Fairmaire (Coleoptera: Tenebrionidae)

Abstract

The first continuous cell line from the neonate larval tissues of Blaps rhynchoptera, which has been used as a folk medicine in Yunnan Province, China, was established and designated RIRI-BR1. This cell line was serially subcultured in Schneider's medium supplemented with 15% fetal bovine serum (FBS). The cells grew adherent to culture flasks and exhibited spindle-like and polygonal shapes. The growth rate was determined at the 50th passage, and the population doubling time was calculated to be 79.5 h. The post-thaw viability of the cell line at different passages showed that the cells from higher passages could be recovered easier after cryopreservation than the cells from lower passages. The average chromosome numbers from cells of the RIRI-BR1 cell line at passages 5 to 50 ranged from 12 to 130. The rDNA internal transcribed spacer (ITS) sequence analysis indicated that the RIRI-BR1 cell line was derived from B. rhynchoptera.

Real‐time cell analysis and heat shock protein gene expression in the TcA Tribolium castaneum cell line in response to environmental stress conditions

“…Huhtala et al (2005) studied the production of heat shock proteins in Spodoptera frugiperda cells in response to heat shock, salinity, pH changes, alcohol, and oxygen deprivation conditions. Coleoptera cell lines are increasingly being used on different studies; Anthonomus grandis (McIntosh et al, 1992), Leptinotarsa decemlineata (Lynn, 1995;Long et al, 2002), Xylotrechus pyrrhoderus (Iwabuchi, 1999), Plagionotus christophi (Hoshino et al, 2009), or Blaps rhynchoptera (Zhang et al, 2015).…”