2010
DOI: 10.1007/s00775-010-0658-6
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A new CuZ active form in the catalytic reduction of N2O by nitrous oxide reductase from Pseudomonas nautica

Abstract: The final step of bacterial denitrification, the two-electron reduction of N 2 O to N 2 , is catalyzed by a multicopper enzyme named nitrous oxide reductase. The catalytic centre of this enzyme is a tetranuclear copper site called CuZ, unique in biological systems. The in vitro reconstruction of the activity requires a slow activation in the presence of the artificial electron donor, reduced methyl viologen, necessary to reduce CuZ from the resting nonactive state (1Cu II /3Cu I ) to the fully reduced state (4… Show more

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Cited by 27 publications
(63 citation statements)
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“…(a). This k ET value is comparable to those reported from AcPAzAcNiR (6.66× 10 5 M −1 s −1 at pH 6.0) [29] and from cytc 552 -MhN 2 OR (5.60× 10 5 M −1 s −1 at pH 7.0) [52].…”
Section: Direct Catalytic Electrochemical Current Via Electron Transfsupporting
confidence: 89%
“…(a). This k ET value is comparable to those reported from AcPAzAcNiR (6.66× 10 5 M −1 s −1 at pH 6.0) [29] and from cytc 552 -MhN 2 OR (5.60× 10 5 M −1 s −1 at pH 7.0) [52].…”
Section: Direct Catalytic Electrochemical Current Via Electron Transfsupporting
confidence: 89%
“…A highly detailed approach for such studies has been laid out (Dell ' Acqua et al, 2012 ). The discovery of the spectroscopic signature of a new intermediate in N 2 OR catalysis in M. hydrocarbonoclasticus , the Cu Z 0 state with characteristic 680 nm absorption, may be of high relevance (Dell ' Acqua et al, 2010 ), as well as the possibility to generate recombinant enzyme and construct new point mutations (Fujita et al , 2007 ). Finally, the possibility to observe bound substrate at an intact Cu Z center opens a path toward depicting actual reaction intermediates, inhibitor complexes, and possibly even the very process of degradation from Cu Z to Cu Z *.…”
Section: Structural and Spectral Features Of The Cu Z Sitementioning
confidence: 99%
“…This intermediated was named CuZ°and is characterized by an absorption band at 680 nm [90]. This intermediate form is different from CuZ*, which is characterized by an absorption band at 640 nm, although the oxidation state of the CuZ center is proposed to be the same [1Cu 2?…”
Section: Catalytic Properties Of Cuz and Reaction Intermediatesmentioning
confidence: 99%