This paper presents a new miniaturized microstrip planar tri-band antenna operating at 2.45 GHz and 5.8 GHz for RFID applications, and 3.5 GHz for WiMAX applications. The proposed antenna is designed by using the slot inserted in its patch, and Complementary Triangular Split Ring Resonator (CTSRR) and Defected Ground Structures (DGS) techniques inserted in its ground plan. The proposed techniques for this antenna is studied, optimised and designed on FR4-epoxy substrate with thickness h=1.6mm, dielectric constant 4.4, and loss tangent 0.025, and simulated by using CST microwave studio software. The simulated results offer a good performances in terms of matching and radiation pattern. The proposed antenna is fabricated on the FR-4 epoxy substrate and tested. A good agreement is found between measured and simulated results. The advantages of this antenna are planar, smaller in size, simple structure, three bands, low in cost and easy to be fabricated.