A New Diagnostic Method for Rapid Detection of Lymph Node Metastases Using a One-Step Nucleic Acid Amplification (OSNA) Assay in Endometrial Cancer

Nagai, Tatsuo; Niikura, Hitoshi; Okamoto, Satoshi; Nakabayashi, Kadzuki; Matoda, Maki; Utsunomiya, Hiroki; Nagase, Satoru; Watanabe, Mika; Takeshima, Nobuhiro; Yaegashi, Nobuo

doi:10.1245/s10434-014-4038-2

Cited by 44 publications

(32 citation statements)

References 24 publications

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“…CK19 is the most common diagnostic marker of LN micro-metastasis in gynecological malignant tumors (7,34,35). Our study revealed that, in the primary lesion and metastatic LNs, the positive expression rate of HPA was over 80% and no less than the rate of CK19, which supported the theory that HPA could be a promising biomarker of LN metastasis in cervical cancer.…”

Section: Discussionsupporting

confidence: 82%

Significance of heparanase in metastatic lymph nodes of cervical squamous cell cancer

Wang

Shi

et al. 2017

Oncology Letters

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Abstract. The aim of this study was to explore the expression of heparanase (HPA) in metastatic lymph nodes (LNs) of cervical cancer and to evaluate HPA as a marker of micro-metastasis of LNs. Immunohistochemistry was performed to detect the expression of HPA in 53 cases with metastasis of LNs (group A) and 49 cases without (group B). Scoring was determined based on the intensity of immuno staining and the size of the staining area. Three points or higher score was considered as positive. Among all cases, the positive rate of HPA was 76.5% in primary lesions and 84.9% in both primary lesions and metastatic LNs in group A. In group B, the rates were 67.3% in primary lesions and 8.2% in metastatic LNs. The expression of HPA in group A was significantly higher than that in group B (P<0.05). Compared with stage IA-IB and well-differentiated and non-metastatic LNs, the LNs of stage IIA and moderately/poorly differentiated and metastatic LNs expressed higher HPA (P<0.05). The overall 5-year survival rate was 73.3% and the median overall survival time (MOS) was 49.0 months. The MOS of the two groups was 36.0 and 58.5 months, respectively (P=0.023); the MOS of patients with positive HPA expression was distinctly lower than that of negative patients (P=0.040). Clinical staging, degree of differentiation, lymph node metastasis and expression of HPA notably affected patient prognosis; lymph node metastasis and expression of HPA were independent risk factors affecting patient prognosis (P<0.05). Our study demonstrated that high-level expression of HPA in cervical cancer was involved in LN metastasis, further impacting on patients' long-term survival. The clinical value of HPA requires further in-depth study.

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Section: Discussionsupporting

confidence: 82%

Significance of heparanase in metastatic lymph nodes of cervical squamous cell cancer

Wang

Shi

et al. 2017

Oncology Letters

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“…Concordance of the OSNA method and the standard histopathological examination was 97.1%. 15 A detailed ultrastaging method of the processing of sentinel lymph node parts would further improve the comparison of both the methods, including the detection of phenomena comprising micrometastases, ITC or benign epithelial inclusion.…”

Section: Discussionmentioning

confidence: 99%

One‐step nucleic acid amplification vs ultrastaging in the detection of sentinel lymph node metastasis in endometrial cancer patients

Kosťun

Pešta

Sláma

et al. 2018

Journal of Surgical Oncology

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Background and Objectives Utilisation of the one‐step nucleic acid amplification (OSNA) molecular biology method for the detection of the metastatic involvement of sentinel lymph nodes (SLNs) in endometrial cancer (EC) patients. A comparison with histopathological ultrastaging and a description of the clinical consequences. Methods Surgically treated EC patients underwent detection of SLNs. Nodes greater than 5 mm were cut into sections 2‐mm thick parallel to the short axis of the node. Odd sections were examined according to the OSNA method, while even ones according to an appropriate ultrastaging protocol. Nodes less than or equal to 5 mm were cut into halves along the longitudinal axis with one half examined according to the OSNA method and the other half by ultrastaging. Results Fifty‐eight patients were included and 135 SLNs were acquired. Both ultrastaging and OSNA agreed on 116 results. According to the OSNA method, 20.69% more patients were classified into International Federation of Gynecology and Obstetrics (FIGO) stage III. When comparing the results of the OSNA method to the conclusions of ultrastaging as a reference method, sensitivity of 90.9%, specificity of 85.5% and concordance of 85.9% were attained. Conclusions The results of the OSNA method showed a higher frequency of detection of micrometastases and included 20.69% more patients into FIGO stage III.

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“…Previous studies [17, 18] have shown that the CK19 copy number for metastasis-negative LN is considerably lower than that for positive LN. In our study, all pathologically positive LN showed an obviously higher level of copy number compared with negative LN.…”

Section: Discussionmentioning

confidence: 98%

“…Nagai et al [18] reported that CK19 protein expression was observed in 98% of primary tumors. Moreover, both type 1 and type 2 tumors showed CK19 protein expression.…”

Section: Discussionmentioning

confidence: 99%

Quantitative RT-PCR Assay for Detecting Lymph Node Metastasis in Endometrial Cancer: A Preliminary Study

et al. 2018

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Objective: The detection accuracy of sentinel lymph node (SLN) metastasis by quantitative reverse transcriptase polymerase chain reaction (qRT-PCR) for endometrial cancer (EC) remains unclear and was assessed in this preliminary study. Methods: We studied primary cancer tissues and pelvic lymph nodes (PLN) from 105 patients with EC. qRT-PCR assay was performed to determine the copy numbers of CK19 mRNA in EC tissues, and negative and positive LN samples. Further, qRT-PCR results were compared with pathological findings. Results: CK19 mRNA expression was detected in 98% (104/106) of the tumors, with a median copy number of 3.0 × 10⁵/μL. Twelve LN were diagnosed as positive by pathological examination. The median copy number of CK19 mRNA for positive and negative LN was 8.1 × 10⁴/μL and 90.4/µL, respectively. CK19 mRNA expression was higher in pathologically positive LN than in pathologically negative LN (p < 0.01); the pathological and qRT-PCR findings showed no discrepancy. When the cutoff value was set at 4,500 copies/µL, qRT-PCR assay using CK19 mRNA exhibited high sensitivity and specificity. Conclusions: Our results demonstrated that qRT-PCR assay, using CK19 mRNA, exhibits a high accuracy for detecting LN metastasis in EC and represents a useful alternative to conventional pathological diagnosis of EC.

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A New Diagnostic Method for Rapid Detection of Lymph Node Metastases Using a One-Step Nucleic Acid Amplification (OSNA) Assay in Endometrial Cancer

Abstract: The study data demonstrated that an OSNA assay using CK19 mRNA was applicable for detecting LN metastases in EC. Combined analysis using an OSNA assay and SLNs may improve individualized treatments according to LN metastatic status.

Cited by 44 publications

References 24 publications

Significance of heparanase in metastatic lymph nodes of cervical squamous cell cancer

Significance of heparanase in metastatic lymph nodes of cervical squamous cell cancer

One‐step nucleic acid amplification vs ultrastaging in the detection of sentinel lymph node metastasis in endometrial cancer patients

Quantitative RT-PCR Assay for Detecting Lymph Node Metastasis in Endometrial Cancer: A Preliminary Study

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