A new embedded biosensor platform based on micro-electrodes array (MEA) technology

Scognamiglio, Viviana; Pezzotti, Ittalo; Pezzotti, Gianni; Cano, Juan Bernardo; Manfredonia, Ivano; Buonasera, Katia; Rodio, Giuseppe; Giardi, Maria Teresa

doi:10.1016/j.snb.2012.09.101

Cited by 26 publications

(14 citation statements)

References 31 publications

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“…These efforts continued in the last decade searching for new mutants with enhanced sensitivity or resistance to herbicides to be included in portable instruments [ 67 , 71 ]. To widen the purpose of analysis and to address the detection of multiple analytes, photosynthetic biosensors were coupled with other enzyme biosensors.…”

Section: Detection Of Photosynthesis-inhibiting Herbicidesmentioning

confidence: 99%

“…To widen the purpose of analysis and to address the detection of multiple analytes, photosynthetic biosensors were coupled with other enzyme biosensors. Moreover, electrochemical and optical modules have been included on the same detection platform, for example, in the multielectrode array for analyzing sugars, phenols, and pesticides reported by Scognamiglio et al [ 71 ]. Researchers investigated new immobilization methods (e.g., laser-induced forward transfer-LIFT [ 70 ], layer-by-layer [ 62 ]) for the purpose of achieving fast, reproducible modification of sensing interfaces with photosynthetic bioelements in a nondestructive manner.…”

Section: Detection Of Photosynthesis-inhibiting Herbicidesmentioning

confidence: 99%

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Advances in Enzyme-Based Biosensors for Pesticide Detection

et al. 2018

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The intensive use of toxic and remanent pesticides in agriculture has prompted research into novel performant, yet cost-effective and fast analytical tools to control the pesticide residue levels in the environment and food. In this context, biosensors based on enzyme inhibition have been proposed as adequate analytical devices with the added advantage of using the toxicity of pesticides for detection purposes, being more “biologically relevant” than standard chromatographic methods. This review proposes an overview of recent advances in the development of biosensors exploiting the inhibition of cholinesterases, photosynthetic system II, alkaline phosphatase, cytochrome P450A1, peroxidase, tyrosinase, laccase, urease, and aldehyde dehydrogenase. While various strategies have been employed to detect pesticides from different classes (organophosphates, carbamates, dithiocarbamates, triazines, phenylureas, diazines, or phenols), the number of practical applications and the variety of environmental and food samples tested remains limited. Recent advances focus on enhancing the sensitivity and selectivity by using nanomaterials in the sensor assembly and novel mutant enzymes in array-type sensor formats in combination with chemometric methods for data analysis. The progress in the development of solar cells enriched the possibilities for efficient wiring of photosynthetic enzymes on different surfaces, opening new avenues for development of biosensors for photosynthesis-inhibiting herbicides.

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Section: Detection Of Photosynthesis-inhibiting Herbicidesmentioning

confidence: 99%

Section: Detection Of Photosynthesis-inhibiting Herbicidesmentioning

confidence: 99%

Advances in Enzyme-Based Biosensors for Pesticide Detection

et al. 2018

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“…In addition, since perm -elective membranes are often deposited by spin coating, the membrane pore size and thickness are difficult to control [6]. Chemical interference by other species that can be oxidized or reduced is also problematic during oxidoreductase-based biosensing of alcohols, phenols, sugars, and metabolic intermediates by amperometric monitoring of either O 2 depletion or H 2 O 2 formation [7][8][9][10][11][12].…”

Section: Introductionmentioning

confidence: 99%

“…Here we report an amperometric Bioelectronic Tongue that contains enzyme electrodes for determination of glucose ascorbic acid, uric acid, and acetaminophen in controlled mixtures, and compare the response of individual sensors with the pattern response of the entire array. While others have reported electrode arrays that include multiple oxidoreductase enzymes, they have analyzed only the individual electrode responses, not the collective array response [7,8,11,12].…”

Section: Introductionmentioning

confidence: 99%

Amperometric Bioelectronic Tongue for glucose determination

Al-Issa

Njagi

Schuckers

et al. 2015

Sensing and Bio-Sensing Research

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a b s t r a c tAn amperometric Bioelectronic Tongue is reported for glucose determination that contains eight sensor electrodes constructed using different metal electrodes (Pt, Au), oxidoreductase enzymes (glucose oxidase, ascorbate oxidase, uricase), and membrane coatings (Nafion, chitosan). The response to varying concentrations of glucose, ascorbic acid, uric acid, and acetaminophen was tested for two models, concentration determination by current density measurements at individual electrodes and concentration determination by a linear regression model for the entire electrode array. The reduced chi-squared for the full array model was found to be about one order of magnitude lower than that for the individualelectrode model. Discrimination of glucose from chemical interference by the other three species is accomplished through a combination of enzyme catalysis, metal electrocatalysis, and membrane surface charge. The benefit of incorporating enzyme electrodes into the sensor array is illustrated by the lower correlation coefficients between different enzyme electrodes relative to non-enzyme coated electrodes. This approach can be more generally applied to detection of other substrates of oxidoreductase enzymes.

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“…An array of sensors, in this way, offer better functionality than is common with traditional arrangements. This could help to overcome the limited use of biosensors, that occurs due to the lack of ability to sensing a specific analyte among various compounds in a same real sample [112]. The multimodal array of enzymatic biosensors (MAEB) is a device built using the functionalized PANI thin films as sensing stages connected to an EGFET transduction stage, in a system adapted to environmental changes.…”

Section: Biosensors Based On Pani Thin Filmmentioning

confidence: 99%

Solid-state biosensors and field-effect transistor devices based on organic semiconductors

Mello¹

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I would like to express my sincere and eternal gratitude to those who made this thesis possible. I am deeply grateful to my supervisor Professor Marcelo Mulato, who supported this work and encourage me to go beyond my own limits every second. His friendship made the path easier. I also express my gratitude to Professor Emil List-Kratochvil from Humboldt-Universität zu Berlin for receiving me on his laboratory. His warm welcome along with a dedicated guidance made my experience abroad enriching and fascinating, leading to excellent results.

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A new embedded biosensor platform based on micro-electrodes array (MEA) technology

Cited by 26 publications

References 31 publications

Advances in Enzyme-Based Biosensors for Pesticide Detection

Advances in Enzyme-Based Biosensors for Pesticide Detection

Amperometric Bioelectronic Tongue for glucose determination

Solid-state biosensors and field-effect transistor devices based on organic semiconductors

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