A new enzyme-linked immunosorbent assay system for human hepatic triglyceride lipase

Miyashita, Kazuya; Kobayashi, Junji; Imamura, Shigeyuki; Kinoshita, Noriaki; Stanhope, Kimber L.; Havel, Peter J.; Nakajima, Katsuyuki; Machida, Tetsuo; Sumino, Hiroyuki; Nara, Makoto; Murakami, Masami

doi:10.1016/j.cca.2013.06.016

Cited by 13 publications

(8 citation statements)

References 25 publications

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“…The current findings that PHP-HTGL concentration showed a tendency toward inverse correlations with serum HDL-C (R = 0.285) were comparable with our previous study (11). On the other hand, there were no significant correlations of serum HTGL with HDL-C, which was an unexpected finding.…”

Section: Discussionsupporting

confidence: 88%

“…The results of immunoprecipitation analysis with MoAbs indicated the presence of HTGL in serum. Previously, we reported an ELISA system for measuring PHP-HTGL using two distinct MoAbs raised against recombinant human HTGL, which was not able to detect serum HTGL (11). However, it is labor intensive to collect large numbers of PHP samples in daily clinical practice.…”

Section: Discussionmentioning

confidence: 99%

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A new enzyme-linked immunosorbent assay system for human serum hepatic triglyceride lipase

Miyashita

Nakajima

Fukamachi

et al. 2017

Journal of Lipid Research

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There is no established method for measuring human hepatic triglyceride (TG) lipase (HTGL) concentration in serum. In this study, we developed new monoclonal Abs (MoAbs) (9A1 mouse MoAb and 141A1 rat MoAb) that react with HTGL both in serum and in postheparin plasma (PHP) and established a novel ELISA system for measuring serum HTGL and PHP-HTGL concentrations. To confirm the specificity of MoAbs, we performed immunoprecipitation-immunoblotting analysis. Both 9A1 mouse MoAb and 141A1 rat MoAb were able to immunoprecipitate not only recombinant HTGL and PHP-HTGL but also serum HTGL, demonstrating that HTGL exists in serum obtained without heparin injection. This method yielded intra- and interassay coefficients of variation of <6% and showed no cross-reactivity with LPL or endothelial lipase. In clinical analysis on 42 male subjects with coronary artery disease, there were strong positive correlations of serum HTGL concentration to PHP-HTGL concentration ( = 0.727, < 0.01). Serum HTGL concentrations showed positive correlations to serum TGs ( = 0.314, < 0.05) and alanine aminotransferase ( = 0.406, < 0.01), and tendencies toward positive correlations to LDL cholesterol, small dense LDL, and γGTP. These results suggest that this new ELISA method for measuring serum HTGL is applicable in daily clinical practice.

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Section: Discussionsupporting

confidence: 88%

Section: Discussionmentioning

confidence: 99%

A new enzyme-linked immunosorbent assay system for human serum hepatic triglyceride lipase

Miyashita

Nakajima

Fukamachi

et al. 2017

Journal of Lipid Research

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“…A report by authors from USA showed that unlike wild type mice, HL KO mice showed no development of fat accumulation in the liver even after high fat diet being loaded 68) . This finding suggests that HL is not simply associated with the development of fatty liver but may have causal effect bolic disorders, and we found that there was a positive correlation between EL and HL concentrations 34) .…”

Section: Hl In Various Conditionssupporting

confidence: 55%

Hepatic Lipase: a Comprehensive View of its Role on Plasma Lipid and Lipoprotein Metabolism

Kobayashi

Miyashita

Nakajima

et al. 2015

J Atheroscler Thromb

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Hepatic lipase (HL) is a key enzyme catalyzing the hydrolysis of triglycerides (TG) and phospholipids (PLs) in several lipoproteins. It is generally recognized that HL is involved in the remodeling of remnant, low-density lipoprotein (LDL), high-density lipoprotein (HDL) and the production of small, dense low-density lipoproteins (sd-LDLs). On the other hand, it is unclear whether HL accelerates or retards atherosclerosis. From the clinical point of view, HL deficiency may provide useful information on answering this question, but the rarity of this disease makes it impossible to conduct epidemiological study. In this review, we describe a comprehensive and updated view of the clinical significance of HL on lipid and lipoprotein metabolism.

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“…The post-heparin plasma was withdrawn 15 min after intravenous injection of 50 units of heparin/kg body weight (BW) for the assay of LPL along with the HTGL activity and concentration. The pre-heparin LPL and HTGL mass were determined with a highly sensitive and specific ELISA [20,21]. The LPL and HTGL activities were determined with the automated assay [22].…”

Section: Measurements Of Lipids Lipoproteins and Lpl Along With The mentioning

confidence: 99%

Lipoprotein lipase does not increase significantly in the postprandial plasma

Ishiyama

Sakamaki

Shimomura

et al. 2017

Clinica Chimica Acta

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A new enzyme-linked immunosorbent assay system for human hepatic triglyceride lipase

Abstract: These results indicate that this new sandwich ELISA for measuring HTGL concentration in PHP can be applied in a daily clinical practice.

Cited by 13 publications

References 25 publications

A new enzyme-linked immunosorbent assay system for human serum hepatic triglyceride lipase

A new enzyme-linked immunosorbent assay system for human serum hepatic triglyceride lipase

Hepatic Lipase: a Comprehensive View of its Role on Plasma Lipid and Lipoprotein Metabolism

Lipoprotein lipase does not increase significantly in the postprandial plasma

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