A new era of stem cell and developmental biology: from blastoids to synthetic embryos and beyond

Kim, Yunhee; Kim, Inha; Shin, Kunyoo

doi:10.1038/s12276-023-01097-8

Cited by 10 publications

(2 citation statements)

References 113 publications

Supporting

Mentioning

Contrasting

Order By: Relevance

“… 73 Additionally, the natural homing capabilities of stem cells enable them to migrate directly to sites of inflammation, damage, or tumors post-administration, which has been gathering increased attention, especially in the design of next-generation products. 74 …”

Section: Hydrogels For Cell Therapymentioning

confidence: 99%

Harnessing the potential of hydrogels for advanced therapeutic applications: current achievements and future directions

Lu,

Ruan,

Huang

et al. 2024

Sig Transduct Target Ther

View full text Add to dashboard Cite

The applications of hydrogels have expanded significantly due to their versatile, highly tunable properties and breakthroughs in biomaterial technologies. In this review, we cover the major achievements and the potential of hydrogels in therapeutic applications, focusing primarily on two areas: emerging cell-based therapies and promising non-cell therapeutic modalities. Within the context of cell therapy, we discuss the capacity of hydrogels to overcome the existing translational challenges faced by mainstream cell therapy paradigms, provide a detailed discussion on the advantages and principal design considerations of hydrogels for boosting the efficacy of cell therapy, as well as list specific examples of their applications in different disease scenarios. We then explore the potential of hydrogels in drug delivery, physical intervention therapies, and other non-cell therapeutic areas (e.g., bioadhesives, artificial tissues, and biosensors), emphasizing their utility beyond mere delivery vehicles. Additionally, we complement our discussion on the latest progress and challenges in the clinical application of hydrogels and outline future research directions, particularly in terms of integration with advanced biomanufacturing technologies. This review aims to present a comprehensive view and critical insights into the design and selection of hydrogels for both cell therapy and non-cell therapies, tailored to meet the therapeutic requirements of diverse diseases and situations.

show abstract

Section: Hydrogels For Cell Therapymentioning

confidence: 99%

Harnessing the potential of hydrogels for advanced therapeutic applications: current achievements and future directions

Lu,

Ruan,

Huang

et al. 2024

Sig Transduct Target Ther

View full text Add to dashboard Cite

show abstract

“…For years, gastrulation was long defined as the formation of three largely homogeneous populations: mesoderm, ectoderm, and endoderm. However, high-resolution technologies and an emerging number of embryonic stem cell models 1,2 unveil an unprecedented heterogeneity among the early germ layers 3,4 . This suggests that much more remains to be uncovered about the mechanisms regulating the distinct cell populations of the gastrula.…”

Section: Introductionmentioning

confidence: 99%

Single-cell RNA sequencing of mutant whole mouse embryos: from the epiblast to the end of gastrulation

Abraham,

Zubillaga,

Roule

et al. 2024

Preprint

View full text Add to dashboard Cite

Over the last decade, single-cell approaches have become the gold standard for studying gene expression dynamics, cell heterogeneity, and cell states within samples. Before single-cell advances, the feasibility of capturing the dynamic cellular landscape and rapid cell transitions during early development was limited. In this paper, we designed a robust pipeline to perform single-cell and nuclei analysis on mouse embryos from E6.5 to E8, corresponding to the onset and completion of gastrulation. Gastrulation is a fundamental process during development that establishes the three germinal layers: mesoderm, ectoderm, and endoderm, which are essential for organogenesis. Extensive literature is available on single-cell omics applied to WT perigastrulating embryos. However, single-cell analysis of mutant embryos is still scarce and often limited to FACS-sorted populations. This is partially due to the technical constraints associated with the need for genotyping, timed pregnancies, the count of embryos with desired genotypes per pregnancy, and the number of cells per embryo at these stages. Here, we present a methodology designed to overcome these limitations. This method establishes breeding and timed pregnancy guidelines to achieve a higher chance of synchronized pregnancies with desired genotypes. Optimization steps in the embryo isolation process coupled with FAST genotyping protocol (3 hours) allow for microdroplet-based single-cell to be performed on the same day, ensuring the high viability of cells and robust results. We also include guidelines for optimal nuclei isolations from embryos. Thus, these approaches increase the feasibility of single-cell approaches of mutant embryos at the gastrulation stage. We anticipate this method will facilitate the analysis of how mutations shape the cellular landscape of the gastrula.

show abstract

Research progress on application of dental stem cells in alveolar bone and periodontal regeneration

Liu,

Guo,

Guo

et al. 2024

MedComm – Future Medicine

View full text Add to dashboard Cite

Periodontal disease is a prevalent inflammatory condition affecting the tissues and bone surrounding the teeth, posing significant challenges to oral health. Current treatments often fall short due to incomplete removal of bacteria and plaque, irreversible damage to gums and bone, and their cost and time‐consuming nature. Stem cell therapy offers a promising alternative that potentially addresses these limitations. This review delves into the biology, mechanisms, and applications of dental stem cells (DSCs) in alveolar bone and periodontal regeneration. We begin with an overview of various stem cell types and their characteristics, highlighting their role in tissue regeneration and the mechanisms involved. Subsequently, we examine preclinical and clinical studies that demonstrate the efficacy and safety of DSC therapy, showcasing their potential in restoring periodontal health. The discussion also extends to the critical challenges and future directions in the field, emphasizing the necessity for standardized protocols and rigorous research to fully integrate DSC‐based therapies into routine clinical practice. This review underscores the transformative potential of DSCs in regenerative dentistry, aiming to provide a comprehensive understanding that can propel further advancements and ultimately improve patient outcomes in periodontal therapy.

show abstract

A new era of stem cell and developmental biology: from blastoids to synthetic embryos and beyond

Cited by 10 publications

References 113 publications

Harnessing the potential of hydrogels for advanced therapeutic applications: current achievements and future directions

Harnessing the potential of hydrogels for advanced therapeutic applications: current achievements and future directions

Single-cell RNA sequencing of mutant whole mouse embryos: from the epiblast to the end of gastrulation

Research progress on application of dental stem cells in alveolar bone and periodontal regeneration

Contact Info

Product

Resources

About