A new European perspective of influenza pandemic planning with a particular focus on the role of mammalian cell culture vaccines

Open-label, randomised, parallel-group, multicentre study to evaluate the safety, tolerability and immunogenicity of an AS03B/oil-in-water emulsion-adjuvanted (AS03B) split-virion versus non-adjuvanted whole-virion H1N1 influenza vaccine in UK children 6 months to 12 years of age

“…In addition, rapid sharing of the results of immunogenicity and postmarketing safety and effectiveness studies among the international community will be essential for allowing to shorten production times, by avoiding the bottleneck of supply of hens' eggs. 12,19 Although substantial safety data regarding the use of trivalent seasonal split and subunit non-adjuvanted inactivated influenza vaccines in children existed, similar safety and efficacy data for novel H1N1 vaccines were lacking.…”

MDCK-SIAT1 Cells Show Improved Isolation Rates for Recent Human Influenza Viruses Compared to Conventional MDCK Cells

“…Infection of immortalized mammalian cell lines in vitro with influenza virus has provided an alternative to egg inoculation (10,13). Following the threat of an avian-derived H5N1 influenza pandemic, vaccine production via cell culture is currently being expanded, allowing more rapid production of influenza vaccine (8,21,29,44).…”

Influenza A viruses: why focusing on M2e-based universal vaccines

“…Theses vaccines are trivalent: containing two subtypes of influenza A and one of influenza B virus, that are using in the world under the different forms of: ''Inactivated whole virion'' vaccine which prepared by treating whole virus particles derived from virus-infected chicken eggs or mammalian cell lines with formalin or b-propiolactone; ''Split virion, inactivated'' or ''disrupted virus'' vaccines containing virus components prepared by treating whole viruses with detergents or organic solvents; ''Surface antigen, inactivated'' vaccines containing highly purified HA and NA antigens; ''Surface antigen, inactivated, virosome'' vaccines containing highly purified HA and NA antigens prepared from disrupted virus particles reconstituted into virosomes with phospholipids [6,16,17]. Live attenuated influenza vaccines are not available in all countries, but countries like Russia, Canada, and USA use it under some condition [18,19].…”