A new flavonoid from the bark of Ulmus pumila L

Cheng, Shengyu; Li, Na; Yu, Yue; Elshafei, Alaa; Jin, Mei; Gao, Li; Zheng, Ming

doi:10.1016/j.bse.2019.103956

Cited by 5 publications

(2 citation statements)

References 27 publications

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“…In addition, most types of elm woods are hard, delicate, wear-resistant, tough, and excellent in quality, and can be used for furniture, construction, and bridges 6 . Numerous beneficial substances can be found in the bark and root bark of elms, many of which have high medicinal value 7 , 8 . The phloem of elm has high viscosity and can be used as a natural plant adhesive, and the leaves can be used as animal feed 9 .…”

Section: Introductionmentioning

confidence: 99%

Complete chloroplast genome structure of four Ulmus species and Hemiptelea davidii and comparative analysis within Ulmaceae species

Liu

Shuxiang³

et al. 2022

Sci Rep

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In this study, the chloroplast (cp) genomes of Hemiptelea davidii, Ulmus parvifolia, Ulmus lamellosa, Ulmus castaneifolia, and Ulmus pumila ‘zhonghuajinye’ were spliced, assembled and annotated using the Illumina HiSeq PE150 sequencing platform, and then compared to the cp genomes of other Ulmus and Ulmaceae species. The results indicated that the cp genomes of the five sequenced species showed a typical tetrad structure with full lengths ranging from 159,113 to 160,388 bp. The large single copy (LSC), inverted repeat (IR), and small single copy (SSC) lengths were in the range of 87,736–88,466 bp, 26,317–26,622 bp and 18,485–19,024 bp, respectively. A total of 130–131 genes were annotated, including 85–86 protein-coding genes, 37 tRNA genes and eight rRNA genes. The GC contents of the five species were similar, ranging from 35.30 to 35.62%. Besides, the GC content was different in different region and the GC content in IR region was the highest. A total of 64-133 single sequence repeat (SSR) loci were identified among all 21 Ulmaceae species. The (A)n and (T)n types of mononucleotide were highest in number, and the lengths were primarily distributed in 10–12 bp, with a clear AT preference. A branch-site model and a Bayes Empirical Bayes analysis indicated that the rps15 and rbcL had the positive selection sites. Besides, the analysis of mVISTA and sliding windows got a lot of hotspots such as trnH/psbA, rps16/trnQ, trnS/trnG, trnG/trnR and rpl32/trnL, which could be utilized as potential markers for the species identification and phylogeny reconstruction within Ulmus in the further studies. Moreover, the evolutionary tree of Ulmaceae species based on common protein genes, whole cp genome sequences and common genes in IR region of the 23 Ulmaceae species were constructed using the ML method. The results showed that these Ulmaceae species were divided into two branches, one that included Ulmus, Zelkova and Hemiptelea, among which Hemiptelea was the first to differentiate and one that included Celtis, Trema, Pteroceltis, Gironniera and Aphananthe. Besides, these variations found in this study could be used for the classification, identification and phylogenetic study of Ulmus species. Our study provided important genetic information to support further investigations into the phylogenetic development and adaptive evolution of Ulmus and Ulmaceae species.

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Section: Introductionmentioning

confidence: 99%

Complete chloroplast genome structure of four Ulmus species and Hemiptelea davidii and comparative analysis within Ulmaceae species

Liu

Shuxiang³

et al. 2022

Sci Rep

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“…The stem and root bark of U. pumila have been used as an anti‐edema, anti‐mastitis, and anti‐inflammation agent in traditional medicine (Wang et al, 2006). Many bioactive flavonoids including catechin and epicatechin, the main constitutional units of condensed tannins, have been identified from the extracts of U. pumila (Cheng et al, 2020). Condensed tannins, also known as proanthocyanidins, are important polyphenolic compounds in nature and our diet, which are formed by a variable number of flavan‐3‐ol subunits and linked through B‐type C–C bonds or A‐type a double interflavan linkage C–C and C–O–C (Hümmer & Schreier, 2008; Song et al, 2017).…”

Section: Introductionmentioning

confidence: 99%

Condensed tannins from Ulmus pumila L. leaves induce G2 /M phase arrest and apoptosis via caspase‐cascade activation in TFK ‐1 cholangiocarcinoma cells

Ren

Qin

Wang

et al. 2022

Journal of Food Biochemistry

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Condensed tannins the polyphenolic compounds that are widespread in plants have been proved to have antitumor potential. Here, we purified the bioactive condensed tannins from leaves of Ulmus pumila L. and explored their structural characteristics, antitumor effect on TFK-1 cholangiocarcinoma cells as well as the related potential mechanism. The UV-Vis, FT-IR spectroscopy, ESI-Full-MS, and thiolysis-HPLC-ESI-MS demonstrated that U. pumila condensed tannins (UCTs) consisted essentially of procyanidins with epicatechin as the main flavan-3-ol extension unit. The UCTs could significantly reduce the survival rate of human cholangiocarcinoma TFK-1, SK-CHA-1, and MZ-CHA-1 cells with the better inhibitory effect on TFK-1 cell proliferation. Flow cytometric assay showed that UCTs affected TFK-1 survival by G2/M phase arrest and inducing apoptosis in a dose-dependent manner. In addition, a total of 6592 differentially expressed genes (DEGs), consisting of 94 upregulated and 6498 downregulated DEGs, were identified between untreated and UCTs-treated TFK-1 cells using RNA-seq technology. Enrichment analysis based on the KEGG database revealed that these DEGs were closely associated with cell cycle and p53 apoptotic signaling pathways. Furthermore, qRT-PCR confirmed that treatment of UCTs to TFK-1 cells caused significant changes in the expression of cyclin E, cdc25 A, cytochrome c, caspase-3, and caspase-8. These results indicated that UCTs exhibited the growth inhibition effect on TFK-1 cells possibly via G2/M cell cycle arrest and activation of caspasecascade to induce apoptosis, and had potential as an anti-cholangiocarcinoma drug for further development. Practical applicationsUlmus pumila L. as a valuable tree species has been widely used in fields of medicine and food. Condensed tannins, the polyphenolic compounds widespread in plants, have been proved to have antitumor potential and be safe to normal cells. In this study, the condensed tannins from leaves of U. pumila (UCTs) remarkably suppressed cholangiocarcinoma (CCA) cell viability possibly via G2/M cell cycle arrest and activation of

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