A new formulated stable papin-pectin aerosol spray for skin wound healing

Abstract

^Äëíê~Åí= A new spray-on topical wound debrider composition consisting of 0.1% w/v papain immobilized in 6% w/v pectin gel was formulated for skin wound healing. The stability of the enzyme activity of this new formulated spray was compared with the 0.1% w/v papain in water solution at the refrigerated temperature of 4 and 75°C. Two aerosol propellants, air and nitrogen, were evaluated for their efficacy. The new formulated spray was then evaluated for its thermal stability under air and N 2 at 75°C. All enzyme activity of papain was measured with a spectrophotometer using casein as a substrate at 37°C and in 0.1 M phosphate buffer at pH 6, as well as trichloral acetic acid to separate the product of casein hydrolysis. All the evaluations showed the new aerosol spray formulation to be far better than the liquid formulation in storage stability and thermal stability at 75°C under atmospheric or higher pressures. The new formulation was tested in a rabbit experiment to evaluate the effectiveness of wound healing. As compared to untreated wound, the formulated spray clearly was superior, yielding a 20% more healing progress in the first 4 days alone. © KSBB hÉóïçêÇëW=é~é~áå=ëí~ÄáäáíóI=éÉÅíáå=ÖÉäI=éêçéÉää~åí=éêÉëëìêÉ=ÉÑÑÉÅí

Development of Hydrogel‐Based Sprayable Wound Dressings for Second‐ and Third‐Degree Burns

“…[ 51 ] Aerosol sprays are more suitable for dressings with enzymatic components, because some of the wound healing enzymes can deactivate in the solution state. [ 57 ] An example aerosol spray, which included papain embedded pectin gel, was reported to soften dead skin and auto‐debride necrotic tissue. [ 57 ] Air or nitrogen can be used as an aerosol propellant for this new spray‐on dressing.…”

“…[ 57 ] An example aerosol spray, which included papain embedded pectin gel, was reported to soften dead skin and auto‐debride necrotic tissue. [ 57 ] Air or nitrogen can be used as an aerosol propellant for this new spray‐on dressing. Nitrogen is superior to air, because air can negatively impact the enzymatic activity in wound healing.…”

“…The thiolate ion groups (SO 2− or SO 3− ) of papain, which serve as active sites for oxidation reactions, can be inactivated by air. [ 57 ] Therefore, the half‐life of nitrogen aerosol is ten times longer than that of spray solutions. In another study, a hydrophilic extract from the Haruan ( Channa striatus ), a kind of Malaysian fish, was used in aerosol sprays.…”

Plasma treatments of dressings for wound healing: a review

“…Acute wounds that require intervention include mechanical abrasions, tear, surgical procedure, burns and chemical injuries. Chronic wounds include diabetic foot ulcers, pressure ulcers and leg ulcers (Boateng et al 2008;Jáuregui et al 2009). In general, acute wounds heal within 3 weeks while chronic wounds persist for more than 12 weeks and often reoccur.…”

Biopolymers as Wound Healing Materials: Challenges and New Strategies

“…The difference was calculated to be significant based on the Student´s t-test method with the resulting p <0.05. It was concluded that papain immobilized in pectin can be used for the development of effective aerosol spray system for wound healing in the areas of enzymatic debridement of necrotic tissue and liquefaction of slough to remove dead or contaminated tissue in acute and chronic lesions, such as diabetic ulcers, pressure ulcers, varicose ulcers, and traumatic infected wounds, postoperative wounds, burns, carbuncles, and pilonidal cyst wounds (Jáuregui et al, 2009). Lyofoam, polyurethane foam, is normally hydrophobic; however, when applying heat and pressure, it becomes hydrophilic and, in this form, while providing blood and exudates absorption, it also prevents drying the wound surface completely (Johnson et al, 1998;Catarino et al, 2000;Fenn & Butler, 2001;Lehnert & Jhala, 2005).…”