A new fungus-mediated RNAi method established and used to study the fatty acid and retinol binding protein function of the plant-parasitic nematode <i>Aphelenchoides besseyi</i>

Ding, S.; Wang, Dongwei; Xu, Chunling; Yang, Si-Hua; Cheng, Xi; Peng, Xiaofang; Chen, Chun; Xie, Hui

doi:10.1080/15476286.2020.1852779

Cited by 5 publications

(7 citation statements)

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“…Previous studies have demonstrated that the reproduction and pathogenic effects of RWTN on the host were inhibited by in vitro RNAi and fungal-mediated RNAi of Ab-far-1 and Ab-far-4 [ 12 , 21 ]. It has been confirmed that RWTN infected A. thaliana and caused recognizable symptoms, and proposed a method to determine the pathogenicity and reproduction rates of RWTNs using A. thaliana as a model host plant [ 26 ].…”

Section: Resultsmentioning

confidence: 99%

“…According to the method described by Ding et al [ 21 ], Ab-far-n hpRNA transgenic B. cinerea were obtained and cultured at 25 °C for later use in subsequent experiments. Genomic DNA of transgenic B. cinerea was extracted from the hygromycin-tolerant generation, as described previously [ 40 ].…”

Section: Methodsmentioning

confidence: 99%

“…RNAi is an important method of reverse genetics and has become the most effective tool for studying the function of the PPN gene. In our previous study, we established a fungal-mediated RNAi method for plant nematodes by feeding these organisms the target gene hairpin RNA (hpRNA) transgenic Botrytis cinerea [ 21 ]. Compared with the RNAi effect produced by in vitro RNAi, this method enables persistence and heritability.…”

Section: Introductionmentioning

confidence: 99%

“…Compared with the RNAi effect produced by in vitro RNAi, this method enables persistence and heritability. Compared with in planta RNAi, this method has the advantage of simple operation, high conversion rate, good reproducibility, and a shorter period [ 21 ]. Therefore, the functions and specific roles of the eight Ab-far genes were further studied using fungi-mediated RNAi.…”

Section: Introductionmentioning

confidence: 99%

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Novel Functions of the Fatty Acid and Retinol Binding Protein (FAR) Gene Family Revealed by Fungus-Mediated RNAi in the Parasitic Nematode, Aphelenchoides besseyi

Ding

Chen

et al. 2021

IJMS

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RNA interference (RNAi) is a powerful tool for the analysis of gene function in nematodes. Fatty acid and retinol binding protein (FAR) is a protein that only exists in nematodes and plays an important role in their life activities. The rice white-tip nematode (RWTN), Aphelenchoides besseyi, is a migratory endoparasitic plant nematode that causes serious damage in agricultural production. In this study, the expression levels of eight RWTN genes were effectively decreased when RWTN was fed Ab-far-n (n: 1–8) hairpin RNA transgenic Botrytis cinerea (ARTBn). These functions of the far gene family were identified to be consistent and diverse through phenotypic changes after any gene was silenced. Such consistency indicates that the body lengths of the females were significantly shortened after silencing any of the eight Ab-far genes. The diversities were mainly manifested as follows: (1) Reproduction of nematodes was clearly inhibited after Ab-far-1 to Ab-far-4 were silenced. In addition, silencing Ab-far-2 could inhibit the pathogenicity of nematodes to Arabidopsis; (2) gonad length of female nematodes was significantly shortened after Ab-far-2 and Ab-far-4 were silenced; (3) proportion of male nematodes significantly increased in the adult population after Ab-far-1, Ab-far-3, and Ab-far-5 were silenced, whereas the proportion of adult nematodes significantly decreased in the nematode population after Ab-far-4 were silenced. (4) Fat storage of nematodes significantly decreased after Ab-far-3, Ab-far-4, and Ab-far-7 were silenced. To our knowledge, this is the first study to demonstrate that Ab-far genes affect sex formation and lipid metabolism in nematodes, which provides valuable data for further study and control of RWTNs.

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Section: Resultsmentioning

confidence: 99%

Section: Methodsmentioning

confidence: 99%

Section: Introductionmentioning

confidence: 99%

Section: Introductionmentioning

confidence: 99%

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Novel Functions of the Fatty Acid and Retinol Binding Protein (FAR) Gene Family Revealed by Fungus-Mediated RNAi in the Parasitic Nematode, Aphelenchoides besseyi

Ding

Chen

et al. 2021

IJMS

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“…Fatty acid and retinol-binding proteins (FAR) are among the most widely studied proteins secreted by the body wall of nematodes, and have been widely reported in free-living nematodes, animal-parasitic nematodes, and plant-parasitic nematodes [ 24 , 25 , 26 , 27 , 28 , 29 , 30 , 31 , 32 , 33 , 34 , 35 , 36 , 37 ]. At present, the structures and functions of FAR are mainly explained as follows: (1) FAR are unique to nematodes and do not exist in other biological groups [ 38 ]; (2) their function in obtaining fatty acids and retinols from hosts and the environment is necessary for nematode lipid biosynthesis and assembly of macromolecular structures to complete the growth and development of nematodes [ 31 , 32 ]; (3) most of them are located on the nematode body wall, which is the host–parasite interface, and, therefore, they play important roles in promoting nematode parasitism and regulating host defences [ 30 ]; and (4) there is evidence that FAR can influence the fecundity and pathogenicity of nematodes [ 32 , 33 , 39 ]. In previous studies, we found eight FAR proteins with significantly different mRNA localisations and diverse functions in RWTNs [ 32 , 33 , 39 ].…”

Section: Introductionmentioning

confidence: 99%

Aphelenchoides besseyi Ab-FAR-1 Interacts with Arabidopsis thaliana AtADF3 to Interfere with Actin Cytoskeleton, and Promotes Nematode Parasitism and Pathogenicity

Ding

Cheng²,

Wang³

et al. 2022

IJMS

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Fatty acid and retinol binding proteins (FAR) are unique proteins found in nematodes and are considered potential targets for controlling these parasites. However, their functions in nematode parasitism and pathogenicity and interaction with hosts are still unclear. In this study, we investigated the specific roles of rice white tip nematodes (RWTNs), Aphelenchoides besseyi, and a protein, Ab-FAR-1, to elucidate the parasitic and pathogenic processes of nematodes. The results showed that the expression level of Ab-far-1 was significantly up-regulated after A. besseyi infection of the plant. The immunofluorescence and subcellular localisation showed that Ab-FAR-1 was secreted into plant tissues mainly through the body wall of nematodes and might act in the nucleus and cytoplasm of plant cells. The pathogenicity of RWTNs was enhanced in Arabidopsis thaliana overexpressing Ab-FAR-1 and inhibited in Ab-far-1 RNAi A. thaliana. Yeast two-hybrid, Co-IP, BiFC, and nematode inoculation experiments showed that Ab-FAR-1 could interact with the A. thaliana actin-depolymerizing factor protein AtADF3, and the A. thaliana adf3 mutant was more susceptible to nematodes. An in vitro actin filament depolymerisation assay demonstrated that Ab-FAR-1 could inhibit AtADF3-mediated depolymerisation of actin filaments, and the turnover process of cellular actin filaments was also affected in A. thaliana overexpressing Ab-FAR-1. In addition, flg22-mediated host defence responses were suppressed in A. thaliana overexpressing Ab-FAR-1 and adf3 mutants. Therefore, this study confirmed that RWTNs can affect the turnover of actin filament remodelling mediated by AtADF3 through Ab-FAR-1 secretion and thus inhibit plant PAMP-triggered immunity (PTI), promoting the parasitism and pathogenicity of nematodes.

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Silencing of the T-type voltage-gated calcium channel α1 subunit by fungus-mediated RNAi altered the structure of F-actin and caused defective behaviors in Ditylenchus destructor

Yang,

Zhou,

Yang

et al. 2024

Mol Biol Rep

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A new fungus-mediated RNAi method established and used to study the fatty acid and retinol binding protein function of the plant-parasitic nematode Aphelenchoides besseyi

Cited by 5 publications

References 57 publications

Novel Functions of the Fatty Acid and Retinol Binding Protein (FAR) Gene Family Revealed by Fungus-Mediated RNAi in the Parasitic Nematode, Aphelenchoides besseyi

Novel Functions of the Fatty Acid and Retinol Binding Protein (FAR) Gene Family Revealed by Fungus-Mediated RNAi in the Parasitic Nematode, Aphelenchoides besseyi

Aphelenchoides besseyi Ab-FAR-1 Interacts with Arabidopsis thaliana AtADF3 to Interfere with Actin Cytoskeleton, and Promotes Nematode Parasitism and Pathogenicity

Silencing of the T-type voltage-gated calcium channel α1 subunit by fungus-mediated RNAi altered the structure of F-actin and caused defective behaviors in Ditylenchus destructor

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