Soil moisture is a key parameter for land-atmosphere interaction system; however, fewer existing spatial-temporally continuous and high-quality observation records impose great limitations on the application of soil moisture on long term climate change monitoring and predicting. Therefore, this study selected the Qinghai–Tibet Plateau (QTP) of China as research region, and explored the feasibility of using Artificial Neural Network (ANN) to reconstruct soil moisture product based on AMSR-2/AMSR-E brightness temperature and SMAP satellite data by introducing auxiliary variables, specifically considering the sensitivity of different combination of input variables, number of neurons in hidden layer, sample ratio, and precipitation threshold in model building. The results showed that the ANN model had the highest accuracy when all variables were used as inputs, it had a network containing 12 neurons in a hidden layer, it had a sample ratio 80%-10%-10% (training-validation-testing), and had a precipitation threshold of 8.75 mm, respectively. Furthermore, validation of the reconstructed soil moisture product (named ANN-SM) in other period were conducted by comparing with SMAP (April 2019 to July 2021) for all grid cells and in situ soil moisture sites (August 2010 to March 2015) of QTP, which achieved an ideal accuracy. In general, the proposed method is capable of rebuilding soil moisture products by adopting different satellite data and our soil moisture product is promising for serving the studies of long-term global and regional dynamics in water cycle and climate.