A new gene nested within the<i>dunce</i>genetic unit of<i>Drosophila melanogaster</i>

Furia, Maria; Digilio, Filomena Anna; Artiaco, Dora; Giordano, Elena; Polito, Lino C.

doi:10.1093/nar/18.19.5837

Cited by 22 publications

(13 citation statements)

References 32 publications

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“…It encodes a salivary gland secreted protein (Muskavitch & Hogness, 1980); the same function has been recently suggested for ng-1 (Furia et al 1990), while Pig-1 function has not yet been clearly established. The three genes are located very close to each other within a DNA segment smaller than 10 kb, with Pig-1 mapping only 813 bp upstream Sgs-4 (Furia et al 1991) and ng-1 mapping about 6 kb downstream Pig-1 (Furia et al 1990). Sgs-4, Pig-1 and ng-1 share common structural and regulatory features, all being small uninterrupted genes that are specifically transcribed within the salivary glands during the larval period of the Drosophila life-cycle.…”

Section: Introductionmentioning

confidence: 82%

“…Throughout the larval stages, however, each gene shows a particular developmental profile. Sgs-4 mRNA is detected at a high level only during the second half of the third instar (Muskavitch & Hogness, 1980) while ng-1 transcript is present at a high level during the entire third instar (Furia et al 1990) and Pig-1 RNA is present at a high level throughout all the larval life, reaching a maximal level at the second instar (Chen et al 1987;Hofmann & Korge, 1987).…”

Section: Introductionmentioning

confidence: 97%

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Effect of ecd¹ mutation on the expression of genes mapped at the Drosophila melanogaster 3C11-12 intermoult puff

et al. 1992

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The Drosophila melanogaster ecd' mutation causes a severe temperature-sensitive deficiency in the titre of the steroid hormone ecdysone. This mutation was used to investigate the role of ecdysone in both the transcription of the genes mapped at the 3C11-12 intermoult puff region and the puff formation. Thoroughly synchronized ecd' larvae were shifted to the non-permissive temperature at various times of the development; after 24 or 48 h, the levels of the transcripts derived from Sgs-4, Pig-1 and ng-1, the three genes located at the 3C11-12 polytene bands, were determined. The results showed that the levels of the transcripts encoded by Pig-1 and ng-1 are unaffected by the drop in the ecdysone titre occurring in non-permissive conditions whereas the amount of Sgs-4 mRNA is greatly reduced. These data clearly indicate that transcription of the three genes mapped within the puff region is affected differently by the hormone. Furthermore, ecd' larvae cultured at the non-permissive temperature show a prominent puff at the 3C11-12 polytene bands, indicating that ecdysone is not essential for puff induction and that puff size is not simply correlated with high-level Sgs-4 transcription.

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Section: Introductionmentioning

confidence: 82%

Section: Introductionmentioning

confidence: 97%

Effect of ecd¹ mutation on the expression of genes mapped at the Drosophila melanogaster 3C11-12 intermoult puff

et al. 1992

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“…5, is unusual but not unprecedented for group III introns. The human factor VII, neurofibromatosis type I and thyroglobulin genes all contain one or more unrelated genes within large introns [34][35][36] and the D. melanogaster dunce gene contains no fewer than three other genes in two introns [37]. Remarkably, the 2.8-kb transcript of haspin is encoded within an intron of only 4.4 kb.…”

Section: Gene Structurementioning

confidence: 99%

Structure, function and evolution of haspin and haspinrelated proteins, a distinctive group of eukaryotic protein kinases

Higgins¹

2003

Cellular and Molecular Life Sciences (CMLS)

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The haspins constitute a newly defined protein family containing a distinctive C-terminal eukaryotic protein kinase domain and divergent N termini. Haspin homologues are found in animals, plants and fungi, suggesting an origin early in eukaryotic evolution. Most species have a single haspin homologue. However, Saccharomyces cerevisiae has two such genes, while Caenorhabditis elegans has at least three haspin homologues and approximately 16 haspin-related genes. Mammalian haspin genes have features of retrogenes and are strongly expressed in male germ cells and at lower levels in some somatic tissues. They encode nuclear proteins with serine/threonine kinase activity. Murine haspin is reported to inhibit cell cycle progression in cell lines. One of the S. cerevisiae homologues, ALK1, is a member of the CLB2 gene cluster that peaks in expression at M phase and thus may function in mitosis. Therefore, the haspins are an intriguing group of kinases likely to have important roles during or following both meiosis and mitosis.

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“…For example, the dunce locus in D. melanogaster has at least three intronic genes (Chen et al 1987;Furia et al 1990). In humans, the neurofibromatosis type 1 gene has three intronic genes (Xu et al 1990), and the factor VIII gene has a single gene in an intron (Levinson et al 1990).…”

Section: Introductionmentioning

confidence: 99%

Unusual organizational features of the Drosophila Gart locus are not conserved within diptera

Clark

Henikoff

1992

J Mol Evol

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The Drosophila Gart locus consists of two genes. One gene encodes three enzymes in the de novo purine nucleotide biosynthesis pathway [glycinamide ribonucleotide synthetase (GARS), aminoimidazole ribonucleotide synthetase (AIRS), and glycinamide ribonucleotide transformylase (GART)]. The second gene lies within an intron of the purine gene and encodes a cuticle protein. To investigate the evolution of the Gart locus, the Chironomus tentans homolog was cloned by screening a genomic DNA library with a polymerase chain reaction product. This study shows that the interesting structural features of this locus conserved in two distant Drosophila species are not found in the Chironomus homolog. These features include the cuticle protein gene nested within an intron and the existence of an alternative transcript to yield a monofunctional enzyme. In addition, the extremely rapid divergence of coding sequence seen for members of the tandemly duplicated AIRS domain in Drosophila is found to be much less rapid in Chironomus.

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A new gene nested within theduncegenetic unit ofDrosophila melanogaster

Cited by 22 publications

References 32 publications

Effect of ecd¹ mutation on the expression of genes mapped at the Drosophila melanogaster 3C11-12 intermoult puff

Effect of ecd¹ mutation on the expression of genes mapped at the Drosophila melanogaster 3C11-12 intermoult puff

Structure, function and evolution of haspin and haspinrelated proteins, a distinctive group of eukaryotic protein kinases

Unusual organizational features of the Drosophila Gart locus are not conserved within diptera

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A new gene nested within theduncegenetic unit ofDrosophila melanogaster

Cited by 22 publications

References 32 publications

Effect of ecd1 mutation on the expression of genes mapped at the Drosophila melanogaster 3C11-12 intermoult puff

Effect of ecd1 mutation on the expression of genes mapped at the Drosophila melanogaster 3C11-12 intermoult puff

Structure, function and evolution of haspin and haspinrelated proteins, a distinctive group of eukaryotic protein kinases

Unusual organizational features of the Drosophila Gart locus are not conserved within diptera

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Effect of ecd¹ mutation on the expression of genes mapped at the Drosophila melanogaster 3C11-12 intermoult puff

Effect of ecd¹ mutation on the expression of genes mapped at the Drosophila melanogaster 3C11-12 intermoult puff