A new genome of Acidithiobacillus thiooxidans provides insights into adaptation to a bioleaching environment

Travisany, Dante; Cortés, María Paz; Latorre, Mauricio; Génova, Alex Di; Budinich, Marko; Fazzini, Roberto A. Bobadilla; Parada, Pilar; González, Maurício; Maass, Alejandro

doi:10.1016/j.resmic.2014.08.004

Cited by 50 publications

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“…As stated by Nuñez et al [18], genome size in prokaryotes is related to metabolic diversity, effective population size, regulatory complexity, and horizontal transfer rates. And larger genomes might have a high adaptive plasticity compared to smaller genomes [17]. Previous studies showed that the genome of A. ferrooxidans DSM 16786 isolated from mining processes [19] was much larger than that of A. ferrooxidans ATCC 23270, which was from the bituminous effluent of coal mine [17].…”

Section: Resultsmentioning

confidence: 99%

“…And larger genomes might have a high adaptive plasticity compared to smaller genomes [17]. Previous studies showed that the genome of A. ferrooxidans DSM 16786 isolated from mining processes [19] was much larger than that of A. ferrooxidans ATCC 23270, which was from the bituminous effluent of coal mine [17]. Likewise, the larger genome size of A. thiooxidans strains GD1-3, DXS-W, and Licanantay obtained from copper mining implied that putative gene acquisition might enable them to adapt to the high concentration of metals in these metal mines.…”

Section: Resultsmentioning

confidence: 99%

“…Acidithiobacillus thiooxidans ( A. thiooxidans ) is an aerobic mesophilic microorganism belonging to the genus Acidithiobacillus and is considered to play an important role in industrial bioleaching [10]. Until recently, three genomes of A. thiooxidans strains including ATCC 19377, A01, and Licanantay from various habitats (Table 1) have been sequenced and submitted to National Center for Biotechnology Information (NCBI) [15,16,17]. When the draft genome sequences were released, numerous genes were annotated and public, thus providing a wealth of valuable information.…”

Section: Introductionmentioning

confidence: 99%

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Comparative Genomics of the Extreme Acidophile Acidithiobacillus thiooxidans Reveals Intraspecific Divergence and Niche Adaptation

Zhang

Feng

Tao

et al. 2016

IJMS

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Acidithiobacillus thiooxidans known for its ubiquity in diverse acidic and sulfur-bearing environments worldwide was used as the research subject in this study. To explore the genomic fluidity and intraspecific diversity of Acidithiobacillus thiooxidans (A. thiooxidans) species, comparative genomics based on nine draft genomes was performed. Phylogenomic scrutiny provided first insights into the multiple groupings of these strains, suggesting that genetic diversity might be potentially correlated with their geographic distribution as well as geochemical conditions. While these strains shared a large number of common genes, they displayed differences in gene content. Functional assignment indicated that the core genome was essential for microbial basic activities such as energy acquisition and uptake of nutrients, whereas the accessory genome was thought to be involved in niche adaptation. Comprehensive analysis of their predicted central metabolism revealed that few differences were observed among these strains. Further analyses showed evidences of relevance between environmental conditions and genomic diversification. Furthermore, a diverse pool of mobile genetic elements including insertion sequences and genomic islands in all A. thiooxidans strains probably demonstrated the frequent genetic flow (such as lateral gene transfer) in the extremely acidic environments. From another perspective, these elements might endow A. thiooxidans species with capacities to withstand the chemical constraints of their natural habitats. Taken together, our findings bring some valuable data to better understand the genomic diversity and econiche adaptation within A. thiooxidans strains.

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Section: Resultsmentioning

confidence: 99%

Section: Resultsmentioning

confidence: 99%

Section: Introductionmentioning

confidence: 99%

See 1 more Smart Citation

Comparative Genomics of the Extreme Acidophile Acidithiobacillus thiooxidans Reveals Intraspecific Divergence and Niche Adaptation

Zhang

Feng

Tao

et al. 2016

IJMS

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“…These toxic effects include blocking of biologically important functional groups and the denaturation of enzymes (Gholami et al, 2011). Travisany et al (2014), report the genome sequence of A. thiooxidans Licanantay, the first strain from a copper mine and was compared with two other A. thiooxidans non-metal mining strains, they determined that these strains share a large core genome of 2109 coding sequences and a high average nucleotide identity over 98%. Nevertheless, the presence of 841 strain-specific genes (absent in other A. thiooxidans strains) suggests a particular adaptation of Licanantay to its specific bio-mining environment.…”

Section: Discussionmentioning

confidence: 99%

Biological treatment of coal combustion wastes by Acidithiobacillus thiooxidans DSM 26636

2018

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The high levels of toxicity generated by the heavy metal content in industrial wastes has generated environmental and health concerns. One of the strategies to reduce the metallic load is the use of sulfur-oxidizing bacteria, due to its ability to produce sulfuric acid involved in the metal leaching. The aim of this research was to evaluate the growth of Acidithioobacillus thiooxidans DSM 26636 and its ability to leach metals from slags and ashes from coal combustion wastes. Microbial growth was monitoring by sulfate and sulfuric acid production. The metal content in slags and fly ashes was determined by ICP-OES. The experiments were carried out during 21 days at 30°C, 150 rpm in 125 mL Erlenmeyer flasks containing 30 mL of Starkey media added with 1% (w/v) of elemental sulfur and 1% (w/v) of slags or ashes. Results showed that Acidithioobacillus thiooxidans was able to leach V, Fe, Mg, Al, Si and Ni from slags. For fly ashes, metal leaching was Al, Ni, Sn, Mg, Zn and Si. Summarizing, Acidithioobacillus thiooxidans could be used for the leaching of different metals contained in wastes from carbon combustion plant. Mexican Journal of Biotechnology 2018, 3(3):54-67 RESUMENLos altos niveles de toxicidad generados por el alto contenido de metales en los residuos industriales han generado problemas ambientales y de salud. Una de las estrategias para reducir la carga metálica en estos residuos podría ser mediante el uso de bacterias sulfooxidantes debido a que tienen la habilidad de oxidar azufre y producir ácido sulfúrico, el cual está implicado en la lixiviación de metales. Así, el objetivo de la presente investigación fue evaluar el crecimiento de Acidithioobacillus thiooxidans DSM26636 y su habilidad de lixiviar metales de escorias y cenizas de una planta de combustión de carbón. El crecimiento microbiano fue monitoreado mediante la producción de sulfatos y ácido sulfúrico. El contenido de metales en las escorias y cenizas se determinó mediante ICP-OES. Los experimentos se llevaron a cabo durante 21 días a 30°C y 150 rpm en matraces Erlenmeyer de 125 mL conteniendo 30 mL de medio Starkey modificado adicionado con azufre elemental al 1%, las cenizas y/o escorias fueron adicionadas al 1%. Los resultados mostraron que Acidithioobacillus thiooxidans fue capaz de lixiviar V, Fe, Mg, Al, Si y Ni de las escorias. En cuanto a las cenizas, los metales lixiviados fueron Al, Ni, Sn, Mg, Zn y Si. En resumen, Acidithioobacillus thiooxidans puede ser utilizada para la lixiviación de metales contenidos en residuos de escorias y cenizas provenientes de una planta de combustión de carbón.

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“…Marinobacter subterrani from an iron mine, and are thus invaluable tools for fundamental investigations into microbial physiology and metabolism 21 . Others are becoming useful tools in biotechnology; for example, an Acidithiobacillus thiooxidans strain isolated from a copper mine that is now being used in industrial bioleaching 22 . Metal-tolerant organisms from mines also Figure 1.…”

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The geomicrobiology of mining environments

Santini

Gagen²

2018

Microbiol. Aust.

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As the global population increases, so does the demand for minerals and energy resources. Demand for some of the major global commodities is currently growing at rates of: copper -1.6% p.a. . This will increase the worldwide extent of mining environments from around 500 000 km 2 at present to 1 330 000 km 2 by 2100, larger than the combined land area of New South Wales and Victoria (1 050 000 km 2 ), making them a globally important habitat for the hardiest of microbial life. The extreme geochemical and physical conditions prevalent in mining environments present great opportunities for discovery of novel microbial species and functions, as well as exciting challenges for microbiologists to apply their understanding to solve complex remediation problems.Major habitats in mining environments can be divided into two main groups (Figure 1): mine sites, where ore is excavated and crushed, including waste storage sites for overburden (rock and soil materials removed to access the ore body), and waste rock (sub-economic rock surrounding the higher grade ore body);and processing/refinery sites, where the ore is upgraded or purified to separate the target element or resource, including waste storage sites for by-products from either aqueous (tailings) or high temperature smelting (slags) refining techniques, and wastewaters from these processes. Not covered in this article are miningaffected environments around mining and refinery sites, which receive inputs from mine sites in the form of dust (ore, overburden, tailings, and the resource product), surface water and groundwater discharges (wastewaters), or even solid wastes (tailings, waste rock) which, in some cases, are exported by riverine or marine disposal. The severity of impacts and disturbance is far lower in mining-affected environments around the site than within the mining or refinery sites that may generate offsite impacts, and we have therefore excluded them from the primary mining environments (mines and refineries) to be discussed here. In Focus MICROBIOLOGY AUSTRALIA *

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A new genome of Acidithiobacillus thiooxidans provides insights into adaptation to a bioleaching environment

Cited by 50 publications

References 63 publications

Comparative Genomics of the Extreme Acidophile Acidithiobacillus thiooxidans Reveals Intraspecific Divergence and Niche Adaptation

Comparative Genomics of the Extreme Acidophile Acidithiobacillus thiooxidans Reveals Intraspecific Divergence and Niche Adaptation

Biological treatment of coal combustion wastes by Acidithiobacillus thiooxidans DSM 26636

The geomicrobiology of mining environments

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