The Ribota site (Ágreda, Soria, Spain) is a new locality in the Matute Formation (Tithonian–Berriasian) composed of several carbonate layers, outstandingly rich in macrovertebrate remains. Fossils show an unusual replacement of the original bioapatite by quartz, and are found as positive reliefs protruding from lacustrine limestone beds. This type of conservation has allowed the identification of around one hundred vertebrate bone accumulations in an outcrop of more than 10 hectares. Osteichthyans (articulated partial skeletons, cranial material, and isolated postcranial bones and scales), crocodylomorphs (disarticulated cranial material, isolated teeth, vertebrae and osteoderms), turtles (partial carapaces and plastra, but also isolated plates) and pterosaurs (cranial and appendicular elements) have been identified. Around 80 specimens have been collected and a preliminary study of part of the collection (35 specimens) has allowed the identification of at least 5 different taxa: Halecomorphi indet., Neoginglymodi indet., Goniopholididae indet., Testudinata indet., and Pterodactyloidea indet. This new site represents one of the few sites from this time interval preserved in a fully lacustrine environment, so these vertebrate assemblages are unique and composed of different animals that presumably lived around and within the lake. They are dominated by aquatic and amphibian vertebrates and was formed by attrition in this lacustrine environment, possibly far from the lake shoreline. These macrovertebrate assemblages provide new data about the diversity in the faunal ecosystems from the Jurassic/Cretaceous transition of the Iberian Basin Rift System.