A new genus of leafhopper subtribe Paraboloponina (Hemiptera: Cicadellidae) with molecular phylogeny of related genera

Abstract

A new leafhopper genus Chandra and species Chandra dehradunensis gen. nov., sp. nov. are described, illustrated from India and placed in the subtribe Paraboloponina (Cidadellidae: Deltocephalinae: Drabescini). This genus is closely associated with the genus Parabolopona Webb but differs in shape of the head, placement of antennae, male genitalia and molecular analysis using Histone H3 and COI genes confirmed the difference. The taxonomic and phylogenetic position of Chandra is discussed using morphological characters and preliminary molecular evidence of the new genus and related genus Parabolopona.

Tribe reassessment of the subhimalayan leafhopper genus Pseudosubhimalus (Homoptera: Cicadellidae) based on molecular phylogeny

“…The PCR protocol for Histone H3 followed Zahniser & Dietrich (2010) and 28S gene was amplified in 25 µl reactions using DNA polymerase (Fermentas GmBH, St. Leon- Rot, Germany) under the following cycling protocol: 4 min. hot start at 94 °C, 35 cycles of denaturation for 30 s at 94 °C, annealing for 60 s at 47 °C, elongation for 50 s at 72 °C and a final extension was carried out at 72 °C for 8 min in a C1000™ Thermal cycler (Meshram, Shashank & Sinha, 2017). The reactions were combined (as described by KOD FX puregene™ manufacturer protocol) of DNA template 4 µl, 2× PCR buffer 12.5 µl, 2 mM dNTP 10 µl, TAQ (KODFX) enzyme 1 unit, and forward and reverse primers were 0.3 µM each at final concentration.…”

A new genus Vittaliana belonging to the tribe Opsiini (Hemiptera: Cicadellidae) from India and its molecular phylogeny

“…Data was collected as previously described in Meshram, Shashank & Sinha (2017) specifically, in and around ICAR research institutes, Vittal, Kasargod (Kerala: India), with a mercury vapor lamp. Hence, no specific permissions were required for any of the collection localities/activities.…”