A new human cell line (FaDu) from a hypopharyngeal carcinoma

Rangan, S. R. S.

doi:10.1002/1097-0142(197201)29:1<117::aid-cncr2820290119>3.0.co;2-r

Cited by 180 publications

(119 citation statements)

References 6 publications

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“…Tumor experiments were performed on a subcutaneous tumor model (FaDu, 5-7 mm in diameter) grown on the thigh of outbred nude mice. FaDu (27) is an established human hypopharyngeal squamous cell carcinoma line that is kept in high passage by the American Type Culture Collection (Rockville, MD, USA). All experiments with animals were performed in accordance with European regulations on the care and use of laboratory animals.…”

Section: Methodsmentioning

confidence: 99%

Spatially localized intermolecular zero‐quantum coherence spectroscopy for in vivo applications

Balla

Melkus

Faber

2006

Magnetic Resonance in Med

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Magnetic resonance spectroscopy (MRS) techniques that use the distant dipolar field (DDF) to locally refocus inhomogeneous line-broadening promise improved spectral resolution in spatially varying fields. We investigated three possible implementations of localized DDF spectroscopy. Theoretical analysis and phantom experiments at 17.6 T showed that only localization immediately prior to acquisition provides sufficient spatial selectivity and sensitivity for in vivo applications. Spectra from an (8 mm) 3 voxel of the rat brain were acquired in 25 min, and three major metabolites were resolved. In a tumor mouse model, DDF spectra with well-resolved lines can be obtained from significantly larger voxels compared to conventional localized spectroscopy. From an inhomogeneous voxel, improved spectral resolution can be obtained with DDF techniques when a sufficient number of increments are sampled along the second spectral dimension. With fewer increments, measurement time is significantly shortened, and DDF techniques can provide higher signal-to-noise ratio ( Key words: iZQC; HOMOGENIZED; localized spectroscopy; inhomogeneous broadening; resolution enhancementIn vivo magnetic resonance spectroscopy (MRS) can be used to resolve spectral patterns to analyze the biochemical composition of tissue. High spectral resolution can be achieved when the magnetic field is extremely homogeneous over the region studied. Such field homogeneity is usually achieved with localized shimming procedures (1,2). If the region of interest (ROI) is strongly structured or includes air/tissue boundaries, homogeneous magnetic fields cannot be obtained and spectral resolution is often poor. One way to avoid inhomogeneous line-broadening caused by small-scale field variations is to use experimental techniques that exploit the effect of the distant dipolar field (DDF) originating from the sample magnetization (3-6). The homogeneity enhancement by intermolecular zeroquantum echo detection (HOMOGENIZED) experiment has been shown to produce narrow line shapes even under temporarily unstable magnetic fields (7), linear inhomogeneities across the sample volume (3-5), and field distortions in living organisms (8,9). Efficient water suppression (WS) and localization of the acquired signal are essential for in vivo applications. While different combinations of WS modules have been described and compared (5,10), the inherently low sensitivity of these methods has hampered the development of localization techniques in vivo (11).In this study we investigate three possible ways to combine localization with the HOMOGENIZED experiment, and demonstrate that high-quality spectra can be obtained in vivo. THEORY Theory of the HOMOGENIZED ExperimentThe HOMOGENIZED pulse sequence is a 2D NMR experiment derived from correlation spectroscopy (COSY) (3,12):The values in brackets indicate the flip angle of RF pulses with pulse phase given in subscript; t 1 is the incremented delay, CG is the correlation magnetic field gradient, and t 2 the acquisition period. The acquire...

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Section: Methodsmentioning

confidence: 99%

Spatially localized intermolecular zero‐quantum coherence spectroscopy for in vivo applications

Balla

Melkus

Faber

2006

Magnetic Resonance in Med

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“…Human HNSCC cell lines including SCC4, OECM-1, 16 FaDu, 17 and HSC-3 cells 18 were purchased from the BCRC (Biosource Collection and Research Center, Taiwan) and JCRB Cell Bank (Osaka, Japan), or kindly provided by Dr KW Chang (National Yang-Ming University, Taipei, Taiwan). SCC4 and OECM-1 cells were maintained in DMEM/F12 and RPMI 1640 medium, respectively.…”

Section: Cell Culturementioning

confidence: 99%

Tyrosine 397 phosphorylation is critical for FAK-promoted Rac1 activation and invasive properties in oral squamous cell carcinoma cells

Yuan

2016

Laboratory Investigation

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Oral squamous cell carcinoma (OSCC) is a common cancer worldwide. Despite advances in diagnosis and therapy, treatment options for patients with metastatic OSCC are few, due in part to the limited understanding of the molecular events involved in the invasion and metastasis of OSCC. In this study, we investigated the expression of focal adhesion kinase (FAK) and its tyrosine 397 phosphorylation (pY397) in the tissue specimens of OSCC. The roles of pY397 in regulating the activities of Rac1 and cortactin and the invasive properties of OSCC cells were further determined. Results from immunohistochemical analyses in 9 benign, 19 premalignant, and 19 malignant oral tissues showed that the immunoreactivity of FAK was observed in 5 benign (56%), 19 premalignant (100%), and 18 malignant tissues (95%), whereas the immunoreactivity of pY397 was only found in 1 of 9 (11%) benign lesions but was observed in 9 premalignant (47%) and 12 malignant (63%) lesions. Compared with the low-invading SCC4 cells, the high-invading OECM-1 cells exhibited higher levels of FAK expression and pY397, correlating with higher levels of GTP-bound Rac1 and cortactin phosphorylation. Manipulation of FAK expression or Y397 phosphorylation in SCC4, FaDu, OECM-1, or HSC-3 cells regulated their Rac1 activities and invasive properties. Furthermore, treatment of NSC23766, a Rac1-specific inhibitor, in OECM-1 and HSC-3 cells led to reduced invasive properties. Nevertheless, knockdown of FAK expression or suppression of pY397 had no effect on the cortactin activity in OECM-1 cells. The data collectively suggest that pY397 plays critical roles in the FAK-promoted Rac1 activation and invasive properties in OSCC cells. Thus, the inhibition of FAK phosphorylation at Y397 or Rac1 activity can serve as a therapeutic strategy for treating patients with metastatic OSCC. Oral squamous cell carcinoma (OSCC) is the most common malignancy occurring in the oral cavity that usually arises from the buccal mucosa and tongue. During the tumor progression, OSCC cells gradually spread and invade to neighboring tissues, including the nasal cavity or the maxillary facial structures, and eventually metastasize to neck lymph nodes and distant organs. 1 Similar to other malignant diseases, tumor metastasis is a great challenge for the treatment of OSCC. This notion is supported by the fact that only 50% of diagnosed OSCC patients can survive for 5 years, and this is mainly because OSCC patients continue to die from tumor relapse at regional or distant sites. 2 Despite advances in diagnosis and therapy in past decades, the promising strategy for treating patients with metastatic OSCC is still underdeveloped, due in part to the limited understanding of the molecular events involved in invasion and metastasis of OSCC. The underlying molecular basis of OSCC metastasis deserves intensive study.Focal adhesion kinase (FAK) is a nonreceptor tyrosine kinase and functionally involves the regulatory processes of multiple cellular events, including adhesion, migration, invasion, prolifer...

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“…JHU-029 (18) cells were a gift of David Sidranski (Johns Hopkins University, Baltimore, Maryland, USA). FaDU (72) and HaCAT (73) cells were a gift of James Rocco (Massachusetts General Hospital). HO1N1 (74), BICR-78 (75), CAL-33 (76), KYSE-30, and KYSE-150 (77) were a gift of S. Michael Rothenberg (Massachusetts General Hospital).…”

Section: Cell Linesmentioning

confidence: 99%

FGFR2 signaling underlies p63 oncogenic function in squamous cell carcinoma

Ramsey

Wilson²,

Ory³

et al. 2013

J. Clin. Invest.

102

101

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Oncogenic transcription factors drive many human cancers, yet identifying and therapeutically targeting the resulting deregulated pathways has proven difficult. Squamous cell carcinoma (SCC) is a common and lethal human cancer, and relatively little progress has been made in improving outcomes for SCC due to a poor understanding of its underlying molecular pathogenesis. While SCCs typically lack somatic oncogene-activating mutations, they exhibit frequent overexpression of the p53-related transcription factor p63. We developed an in vivo murine tumor model to investigate the function and key transcriptional programs of p63 in SCC. Here, we show that established SCCs are exquisitely dependent on p63, as acute genetic ablation of p63 in advanced, invasive SCC induced rapid and dramatic apoptosis and tumor regression. In vivo genome-wide gene expression analysis identified a tumor-survival program involving p63-regulated FGFR2 signaling that was activated by ligand emanating from abundant tumor-associated stroma. Correspondingly, we demonstrate the therapeutic efficacy of extinguishing this signaling axis in endogenous SCCs using the clinical FGFR2 inhibitor AZD4547. Collectively, these results reveal an unanticipated role for p63-driven paracrine FGFR2 signaling as an addicting pathway in human cancer and suggest a new approach for the treatment of SCC.

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A new human cell line (FaDu) from a hypopharyngeal carcinoma

Cited by 180 publications

References 6 publications

Spatially localized intermolecular zero‐quantum coherence spectroscopy for in vivo applications

Spatially localized intermolecular zero‐quantum coherence spectroscopy for in vivo applications

Tyrosine 397 phosphorylation is critical for FAK-promoted Rac1 activation and invasive properties in oral squamous cell carcinoma cells

FGFR2 signaling underlies p63 oncogenic function in squamous cell carcinoma

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