A new hydrophilic interaction liquid chromatographic (HILIC) procedure for the simultaneous determination of pseudoephedrine hydrochloride (PSH), diphenhydramine hydrochloride (DPH) and dextromethorphan hydrobromide (DXH) in cough-cold formulations

Ali, Mohammed Shahid; Ghori, Mohsin; Rafiuddin, Syed; Khatri, Aamer Roshanali

doi:10.1016/j.jpba.2006.06.038

Cited by 54 publications

(26 citation statements)

References 33 publications

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“…Relying on this report, Ali et al [18] found that ion exchange partially contributes to HILIC separation. A similar effect was also investigated by the group of Yoshida [11].…”

Section: The Effect Of Mobile Phasessupporting

confidence: 55%

The advantages of ESI‐MS detection in conjunction with HILIC mode separations: Fundamentals and applications

Nguyen

Schug²

2008

J of Separation Science

258

133

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The analysis of highly hydrophilic, ionic, and polar compounds has been performed by HILIC-ESI-MS for the last few years. The use of low aqueous/high polar organic solvent content in HILIC separation mobile phase is almost ideal for ESI-MS detection in many cases, resulting in increased sensitivity. Although the addition of modifiers such as acids or salts is necessary in some circumstances for a good separation, the optimum concentrations used are still highly amenable for ESI-MS analysis, showing few deleterious effects. In this review, the mechanism of HILIC separation and ESI ion generation will be briefly discussed, followed by a summary of method development and applications in several fields of research including pharmaceutical, biomolecular, food, metabolic, and environmental analysis.

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“…Relying on this report, Ali et al [18] found that ion exchange partially contributes to HILIC separation. A similar effect was also investigated by the group of Yoshida [11].…”

Section: The Effect Of Mobile Phasessupporting

confidence: 55%

The advantages of ESI‐MS detection in conjunction with HILIC mode separations: Fundamentals and applications

Nguyen

Schug²

2008

J of Separation Science

258

133

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“…3,4,5,6,7,8,9 The chromatographic selectivity for compounds using alcohol-based mobile phases differs significantly from that observed with acetonitrile. Also, due to the enhanced polarity of alcohols compared to acetonitrile, the overall retention decreases as well.…”

Section: 2mentioning

confidence: 92%

Characterization of enhanced-fluidity liquid hydrophilic interaction chromatography for the separation of nucleosides and nucleotides

Philibert

Olesik

2011

Journal of Chromatography A

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Hydrophilic Interaction Chromatography (HILIC) is a liquid chromatographic separation mechanism commonly used for polar biological molecules. While nucleotides are very polar analytes, only a few studies have been conducted on their separation using HILIC. Herein, the use of enhanced-fluidity liquid chromatography (EFLC) for the separation of nucleosides and nucleotides under HILIC conditions is investigated. Enhanced-fluidity liquid chromatography involves using common mobile phases with the addition of substantial proportions of a dissolved gas which provides enhanced mobile phase diffusivity and lower viscosity. The impact of varying mobile phase composition: buffer composition, type of base, salt used, salt concentration and mole fraction of CO 2 was studied to provide optimized HILIC separations. Each of these parameters plays a key role in the retention of the analytes, which demonstrates the complexity of the retention mechanism in HILIC. The tailing of phosphorylated compounds was overcome with the use of phosphate buffer and the addition of a strong base; efficiency and peak asymmetry were compared with the addition of either triethylamine (TEA), 1,4-diazabicyclo

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“…One disadvantage of using a high ratio of ACN in the mobile phase is its inability to provide good solubility for polar analytes at higher concentration [4].…”

Section: Mobile Phase Composition Impact On Hilic Selectivitymentioning

confidence: 99%

“…HILIC mobile phase is an aqueous-organic mixture such as water-methanol and water-ACN. The aqueous tetrahdrofuran, isopropanol and ethanol were also reported for HILIC applications [3,4]. Retention mechanism of HILIC is believed to be analyte partitioning between the bulk eluent and the water-rich layer that is partially immobilized on the surface of the stationary phase [1].…”

Section: Introductionmentioning

confidence: 99%

Impact of column temperature and mobile phase components on selectivity of hydrophilic interaction chromatography (HILIC)

Hao¹,

Xiao

Weng

2008

J of Separation Science

248

152

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The retention mechanism and chromatographic behavior for different polar analytes under hydrophilic interaction chromatography (HILIC) conditions have been studied by application of different mobile phases and stationary phases to various analytes at different temperatures. In addition to the commonly accepted mechanism of analyte liquid-liquid partitioning between mobile phase and water-enriched solvent layer which is partially immobilized onto the surface of the stationary phase, hydrogen-bonding, hydrophobic interaction, and ion-exchange interactions may also be involved. The predominant retention mechanism in HILIC separation is not always easily predictable. It can depend not only on the characteristics of the analytes but also on the selection of mobile and stationary phase compositions. The objective of this review is to evaluate the potential application of column temperature and mobile phase composition toward improving HILIC selectivity. The functional groups from analyte structures, stationary phase materials and organic mobile phase solvents will be highlighted.

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A new hydrophilic interaction liquid chromatographic (HILIC) procedure for the simultaneous determination of pseudoephedrine hydrochloride (PSH), diphenhydramine hydrochloride (DPH) and dextromethorphan hydrobromide (DXH) in cough-cold formulations

Cited by 54 publications

References 33 publications

The advantages of ESI‐MS detection in conjunction with HILIC mode separations: Fundamentals and applications

The advantages of ESI‐MS detection in conjunction with HILIC mode separations: Fundamentals and applications

Characterization of enhanced-fluidity liquid hydrophilic interaction chromatography for the separation of nucleosides and nucleotides

Impact of column temperature and mobile phase components on selectivity of hydrophilic interaction chromatography (HILIC)

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