A New <i>in vitro</i> Microsuperfusion Technique for Investigation of Human Detrusor Muscle

Palfrey, E. L. H.; Fry, CH; Shuttleworth, K. E. D.

doi:10.1111/j.1464-410x.1984.tb06134.x

Cited by 22 publications

(15 citation statements)

References 4 publications

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“…Small circular strips of muscle (2 x 0.5 mm) were obtained from segments of human ureter discarded at routine open urological operations. These were mounted in a muscle bath using a method similar to that described by Palfrey et al (1984) and superfused with a modified Tyrode solution at 36°C which had been equilibrated with 95% oxygen and 5% carbon dioxide. The composition of the Tyrode solution was: NaCl 118 mmol/l; KCl 4 mmol/l; NaHCO, 24 mmol/l; NaH2P0, 0.4 mmol/l; MgC126H20 1 mmol/l; CaCl, 1.8 mmol/l; Glucose 6 mmol/l; Na pyrurate 5 mmol/l.…”

Section: Methodssupporting

confidence: 90%

The Action of the Prostaglandins on Isolated Human Ureteric Smooth Muscle

1988

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A study has been carried out on the actions of the prostaglandins E2 and F, , and their synthesis inhibitors, indomethacin and diclofenac sodium, upon isolated human ureteric smooth muscle, using the technique of microsuperfusion designed to ensure good tissue viability. lndomethacin and diclofenac sodium were shown to abolish almost completely the contractile response of ureteric muscle to electrical field stimulation. Contractile activity, in the presence of the inhibitors, could be restored by prostaglandin E, or F2a or by increasing the external potassium concentration, [K+],, of the superfusate.Prostaglandin E, or F , , alone were shown to increase dramatically both the phasic and tonic component of the electrically stimulated contractions, on occasions inducing spontaneous activity.A possible mechanism of action was elucidated with an electrophysiological technique using intracellular microelectrodes. The mean membrane potential recorded was 54.7 mV (SD k 10 mV, n = 15). The depolarising action of raising [K+], was demonstrated and prostaglandin F2,(3 x 10-6M) was shown to produce a small depolarisation of the ureteric muscle cell membrane.

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Section: Methodssupporting

confidence: 90%

The Action of the Prostaglandins on Isolated Human Ureteric Smooth Muscle

1988

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“…Detrusor strips dissected from the bladder contain excitatory nerves which ramify through the muscle mass. Stimulation of such a strip with tetanic trains of short pulses (~< 0.1 ms) stimulates preferentially these nerves to release excitatory transmitters, which cause muscle contraction [51]. In guinea pig detrusor such stimulation evokes an excitatory junction potential (EJP) which, if large enough, can generate an action potential.…”

Section: Non-cholinergic Excitatory Transmittersmentioning

confidence: 99%

Electrophysiological properties of the bladder

Fry

Sui

1998

Int Urogynecol J

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The electrophysiological properties of detrusor smooth muscle are described, in particular with regard to their influence on the contractile properties of the tissue. The Ca2+ and K+ channel activities are most important in generating action potentials, but the role of several other ionic currents is described, including Cl-, Ca2+-activated, stretch-activated and ligand-gated channels. The variable appearance and functions of different ionic currents in disease states is discussed, as well as the question of whether electrical activity can transmit between adjacent smooth muscle cells. In addition, the precise role that electrophysiological phenomena play in the regulation of the contractile state of the smooth muscle cells, as well as the generation of bladder electromyograms, is discussed.

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“…Samples were transported to the laboratory in a Ca‐free Tyrode's solution (see below). Detrusor strips (< 0.5 mm diameter) were dissected from the biopsy after removing the mucosa [10] in Ca‐containing Tyrode's solution. Isolated cells were prepared by dissecting similar detrusor strips in the Ca‐free solution and then subjecting them to enzymatic digestion [11].…”

Section: Methodsmentioning

confidence: 99%

The actions of metabolic inhibition on human detrusor smooth muscle contractility from stable and unstable bladders

Hockey¹,

Wu²,

Fry³

2000

BJU International

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Objectives To determine the important cellular site(s) of action of a brief exposure to NaCN (chosen to reduce mitochondrial respiration and hence mimic cellular hypoxia) on the mechanical properties and regulation of intracellular [Ca 2+ (15)% in the two groups; the reduction in the unstable was signi®cantly less than in the stable group. NaCN similarly reduced the response to 10 mmol/L caffeine, but had no effect on the KCl-induced contraction. NaCN signi®cantly increased the resting sarcoplasmic [Ca 2+ ] and attenuated the calcium transients evoked by carbachol and caffeine, but again had no effect on the KClinduced transient. The reduction of the carbachol calcium transient was also less in cells from unstable bladders than in those from stable bladders. There was no effect of NaCN on intracellular pH, except for a brief, transient alkalosis. Conclusions NaCN reduces both the contraction and Catransient to carbachol by reducing Ca 2+ accumulation by intracellular stores, because the carbachol-and caffeine-evoked responses were similar. Any effect on transmembrane Ca 2+¯u x was minimal because there was no effect on KCl-induced responses. The greater resilience of tissue from unstable bladders to acute cellular hypoxia may re¯ect some adaptation acquired in vivo.

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A New in vitro Microsuperfusion Technique for Investigation of Human Detrusor Muscle

Cited by 22 publications

References 4 publications

The Action of the Prostaglandins on Isolated Human Ureteric Smooth Muscle

The Action of the Prostaglandins on Isolated Human Ureteric Smooth Muscle

Electrophysiological properties of the bladder

The actions of metabolic inhibition on human detrusor smooth muscle contractility from stable and unstable bladders

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