A new in vitro cellular system for the analysis of mineral fiber biopersistence

Nguea, Hermine Dika; Reydellet, Aymon de; Lehuédé, Patrice; Méringo, Alain de; Robé, Anne; Faou, Alain Le; Rihn, Bertrand

doi:10.1007/s00204-007-0257-7

Cited by 12 publications

(7 citation statements)

References 31 publications

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“…The pattern of surface erosion depends on the nature of the fiber, and it was shown to be inversely related to fiber biopersistence in the animal model. Such surface erosion resembled the one observed in fibers from bronchalveolar lavage of exposed rat [57].…”

Section: Svf Degradationsupporting

confidence: 70%

“…For this purpose, besides the techniques described in this review, novel approach should be proposed. Examples include (1) in vitro assays to assess fiber related toxicity, (2) a test that mimics physiological conditions in order to study fiber surface degradation after incubation with macrophages without overloading [57] and study of a fiber component dissolution, compared to control in the same medium without cells, and (3) DNA microarrays chips that would show over-and underexpression of genes in response to fibers, a process that may lead to initiation of a chronic inflammation and thus prediction of eventual fibrogenic or carcinogenic properties of tested fibers [58]. However, the burden is always on the investigator to demonstrate the relevance of results obtained from in vitro studies, preferably by showing that similar results can be obtained using selected in vivo experiments.…”

Section: Resultsmentioning

confidence: 99%

“…Generally, for Si, Fe, and Al, the dissolution of fibers was greater in the flow-through culture system than in culture in wells in the presence of macrophages. However, with Escherichia coliactivated U-937 cells rock wool fiber dissolution occurred and was time-dependent [57]. Fiber degradation depends also on intrinsic properties of macrophages, e.g., expression of ferritin genes, which protein allows Fe uptake [58].…”

Section: Svf Degradationmentioning

confidence: 99%

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Macrophage Culture as a Suitable Paradigm for Evaluation of Synthetic Vitreous Fibers

Nguea¹,

Reydellet²,

Faou³

et al. 2008

Critical Reviews in Toxicology

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The ultimate goal of toxicologic investigation of synthetic vitreous fibers (SVFs) is to provide essential input for the assessment of human risk to their exposure. Toxicity of mineral fibers is usually evaluated by testing biopersistence in rodent model. However, a cellular model would be much appreciated in order to reduce, refine, and replace animal models. Pulmonary disorders triggered by inhalation of occupational or environmental mineral particulates can be the endpoints of a chronic inflammatory process in which alveolar macrophages (AMs) play a crucial role. Depending on the type of SVF involved, phagocytosis of fiber leads to activation of macrophages, resulting in release of fiber components and potent mediators, such as reactive oxygen or nitrogen species and cytokines. As a matter of fact, macrophages should be the cells of choice since SVF toxicity is the consequence of fibers and alveolar macrophages interaction. Today, monocytes and macrophages culture are firmly established as a paradigm in toxicology when several endpoints are assayed in macrophages: (1) fiber durability, (2) fiber surface changes, (3) oxidative stress and genotoxicity in macrophage, and (4) macrophage cell viability and apoptosis. This article is a review of up-to-date knowledge of in vitro studies involving macrophages, and assesses endpoints of macrophage toxicity with an emphasis on (1) dissolution, (2) scanning electron microscopy analysis, (3) cytotoxicity, and (4) gene expression.

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Section: Svf Degradationsupporting

confidence: 70%

Section: Resultsmentioning

confidence: 99%

Section: Svf Degradationmentioning

confidence: 99%

See 1 more Smart Citation

Macrophage Culture as a Suitable Paradigm for Evaluation of Synthetic Vitreous Fibers

Nguea¹,

Reydellet²,

Faou³

et al. 2008

Critical Reviews in Toxicology

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“…For cellular dissolution tests, alveolar macrophages are commonly used 16 , 17 . It must be pointed out that the cellular systems have a number of limitations, since the cells are not in their natural environment and the volumes of used media are small compared to in vivo systems 18 ; (ii) acellular in vitro tests, generally conducted by leaching of specific fibre constituents into the dissolution medium (e.g. simulated lung fluids = SLF), are performed both at pH = 4–4.5 and pH = 7.4 simulating the intracellular phagolysosome 19 and extracellular milieu 20 , respectively.…”

Section: Introductionmentioning

confidence: 99%

In vitro acellular dissolution of mineral fibres: A comparative study

Gualtieri

Pollastri

Gandolfi

et al. 2018

Sci Rep

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The study of the mechanisms by which mineral fibres promote adverse effects in both animals and humans is a hot topic of multidisciplinary research with many aspects that still need to be elucidated. Besides length and diameter, a key parameter that determines the toxicity/pathogenicity of a fibre is biopersistence, one component of which is biodurability. In this paper, biodurability of mineral fibres of social and economic importance (chrysotile, amphibole asbestos and fibrous erionite) has been determined for the first time in a systematic comparative way from in vitro acellular dissolution experiments. Dissolution was possible using the Gamble solution as simulated lung fluid (pH = 4 and at body temperature) so to reproduce the macrophage phagolysosome environment. The investigated mineral fibres display very different dissolution rates. For a 0.25 μm thick fibre, the calculated dissolution time of chrysotile is in the range 94–177 days, very short if compared to that of amphibole fibres (49–245 years), and fibrous erionite (181 years). Diffraction and SEM data on the dissolution products evidence that chrysotile rapidly undergoes amorphization with the formation of a nanophasic silica-rich fibrous metastable pseudomorph as first dissolution step whereas amphibole asbestos and fibrous erionite show minor signs of dissolution even after 9–12 months.

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“…There is the need to investigate in vitro and early-stage in vivo reactions as potential predictive markers for development of lung cancer or mesothelioma; the impact of physico-chemical properties and experimental factors should be considered (Kuempel et al 2017 ). Some mineral fibers may dissolve in the bronchoalveolar lavage, dependent on the specific fiber properties (Nguea et al 2008 ). An evaluation of man-made mineral fibers by the International Agency for Research on Cancer came to the conclusion that epidemiological studies only provide inadequate evidence for carcinogenicity in humans.…”

Section: Toxicity and Ecotoxicity Testing Of Nanomaterialsmentioning

confidence: 99%

Nanomaterials: certain aspects of application, risk assessment and risk communication

et al. 2017

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Development and market introduction of new nanomaterials trigger the need for an adequate risk assessment of such products alongside suitable risk communication measures. Current application of classical and new nanomaterials is analyzed in context of regulatory requirements and standardization for chemicals, food and consumer products. The challenges of nanomaterial characterization as the main bottleneck of risk assessment and regulation are presented. In some areas, e.g., quantification of nanomaterials within complex matrices, the establishment and adaptation of analytical techniques such as laser ablation inductively coupled plasma mass spectrometry and others are potentially suited to meet the requirements. As an example, we here provide an approach for the reliable characterization of human exposure to nanomaterials resulting from food packaging. Furthermore, results of nanomaterial toxicity and ecotoxicity testing are discussed, with concluding key criteria such as solubility and fiber rigidity as important parameters to be considered in material development and regulation. Although an analysis of the public opinion has revealed a distinguished rating depending on the particular field of application, a rather positive perception of nanotechnology could be ascertained for the German public in general. An improvement of material characterization in both toxicological testing as well as end-product control was concluded as being the main obstacle to ensure not only safe use of materials, but also wide acceptance of this and any novel technology in the general public.

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A new in vitro cellular system for the analysis of mineral fiber biopersistence

Cited by 12 publications

References 31 publications

Macrophage Culture as a Suitable Paradigm for Evaluation of Synthetic Vitreous Fibers

Macrophage Culture as a Suitable Paradigm for Evaluation of Synthetic Vitreous Fibers

In vitro acellular dissolution of mineral fibres: A comparative study

Nanomaterials: certain aspects of application, risk assessment and risk communication

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