A new in vitro model of venous hypertension: the effect of pressure on dermal fibroblasts

Healey, Chris; Forgione, Patrick; Lounsbury, Karen M.; Corrow, Kim; Osler, Turner M.; Ricci, Michael A.; Stanley, Andrew

doi:10.1016/s0741-5214(03)00556-1

Cited by 15 publications

(25 citation statements)

References 16 publications

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“…Expression of fibronectin is induced by elevated static pressure that corresponds to hypertension increases 46,47 . Mechanotransduction is highly associated with fibronectin and other interacting proteins, including cytokines that regulate the RhoAdependent response to mechanical stress [48][49][50] .…”

Section: Discussionmentioning

confidence: 99%

Cytoprotective activity of elevated static pressure against oxidative stress in normal human fibroblasts

Kwon

Lee

2011

Mol. Cell. Toxicol.

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DNA damage in normal cells by reactive oxygen species can trigger cellular senescence and tumorigenesis. Therefore, many strategies have been developed to inhibit DNA damage and identify molecules that can prevent various oxidative stress-related diseases, including cancer. To investigate the effect of pressure, preconditioning with two types of atmospheric pressure (hyperbaric mild hyperoxia compressed with air (EP) and hyperbaric normoxia with nitrogen gas (HN)) was applied to normal human lung fibroblasts (WI-38) for 1 or 3 days, and then cells were treated with hydrogen peroxide to induce oxidative damage. Here, we have elucidated two types of pressure that can protect WI-38 cells from oxidative stress-induced DNA damage. To profile the expression of genes that contribute to the cytoprotective effects of the pressures, microarray analyses were performed. Extracellular matrix-, cell adhesion-, and cell cycle-related genes were highly involved in the cytoprotective effects of the pressures. Among the significantly expressed genes, fibronectin was the most inducible in WI-38 cells under the cytoprotective conditions against oxidative damage. Additionally, cellular fibronectin, and not the secreted isoform, acted as a cytoprotective marker at these pressures. Together, these findings suggest that elevated static pressures exhibit cytoprotective activity against oxidative stress-induced DNA damage by inducing cellular fibronectin.

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Section: Discussionmentioning

confidence: 99%

Cytoprotective activity of elevated static pressure against oxidative stress in normal human fibroblasts

Kwon

Lee

2011

Mol. Cell. Toxicol.

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“…In the earlier studies, there were some reports that neonatal fibroblasts grown under elevated static pressure demonstrated inhibited growth rates, increased fibronectin production (Healey et al 2003), and senescent morphologies in an in vitro model (Stanley et al 2005). On the other hand, cardiac growth (hypertrophy of cardiac muscle cell and nonmuscle cell) in rat can be induced by pressure (Dowell and McManus 1978;Teiger et al 1996).…”

Section: Discussionmentioning

confidence: 99%

Comparison of the effects of 40% oxygen and two atmospheric absolute air pressure conditions on stress-induced premature senescence of normal human diploid fibroblasts

Oh¹,

Lee²,

Lee³

et al. 2008

Cell Stress and Chaperones

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The pressure during hyperbaric oxygen treatment may increase oxygen toxicity via an augmented oxygen pressure in the gas. Nevertheless, only a few reports have been published on the effect of cells grown under 2 atmospheric absolute (ATA) pressure. To evaluate the effect of pressure on oxygen toxicity and to study effects in addition to oxygen toxicity, we designed an experiment to compare the effects of normobaric mild hyperoxia (NMH, 40% oxygen) and hyperbaric air condition (HA, air with 2 ATA) on human diploid fibroblasts (HDF) in a hyperbaric incubator. HDFs in both the NMH and the HA condition had a similar oxidative stress response and exhibited premature senescence. To investigate differences in gene profiling in cells grown in the NMH and HA conditions, samples from cells exposed to each condition were applied to microarrays. We found no expression difference in genes related to aging and deoxyribonucleic acid damage, but the expression of genes including cell adhesion, stress response, and transcription were significantly increased in fibroblasts that were responsive to pressure. Among 26 statistically reliable genes, the expression of apoptosis related genes such as ADAM22, Bax, BCL2L14, and UBD, as well as tumor suppressor-related genes like Axin2 and ATF, and also mitogen-activated protein kinase-related genes like mitogen-activated protein kinase kinase kinase 1, histamine receptor, and RAB24, were significantly changed in cells responsive to pressureinduced oxidative stress.

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“…The direct effect of increased tissue pressures on dermal elements can be demonstrated by placing these cells in vitro under high vs. atmospheric pressure. Cells grown under pressure have reduced growth rates, increased fibronectin production, and abnormal morphologies similar to fibroblasts isolated from venous ulcers [104]. Such a model is a useful tool for direct analysis of the influences of venous hypertension on cells.…”

Section: High Pressure Treatmentmentioning

confidence: 99%

Techniques for Wound Healing with a Focus on Pressure Ulcers Elimination

Kuffler¹

2012

TOCVJ

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Pressure ulcers, also called decubitus ulcers, are a common challenge of humanity. They are wounds initiated by pressure on the skin that blocks circulation causing the skin and underlying tissues to die. Without care, pressure ulcers continue to grow in diameter and depth and are exceptionally difficult to heal. They have probably always existed, and are present on Egyptian mummies. Pressure ulcers are a serious health issue, very painful, a significant physiological challenge, can shorten the life of a patient, are an emotional and financial burden to the patient, those who deal with them, and in the United States alone annually costs in excess over $10 billion dollars for care and prolonged hospitalization. Pressure ulcers can develop with as little as two hours of pressure, and because even a brief lapse in avoidance of pressure leads to ulcers, they are frequently impossible to prevent, even for the most conscientious patient with an extensive support group and unlimited financial resources. Therefore, enormous effort is required to find effective and reliable techniques for preventing the initiation of ulcers and eliminating them once they develop. This review looks at the various approaches presently used and in development for the treatment of pressure ulcers, evaluates which may be best in eliminating ulcers, and discusses combinations of techniques that may be most effective in eliminating pressure ulcers.

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A new in vitro model of venous hypertension: the effect of pressure on dermal fibroblasts

Cited by 15 publications

References 16 publications

Cytoprotective activity of elevated static pressure against oxidative stress in normal human fibroblasts

Cytoprotective activity of elevated static pressure against oxidative stress in normal human fibroblasts

Comparison of the effects of 40% oxygen and two atmospheric absolute air pressure conditions on stress-induced premature senescence of normal human diploid fibroblasts

Techniques for Wound Healing with a Focus on Pressure Ulcers Elimination

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