A new in vitro model applied 90Y microspheres to study the effects of low dose beta radiation on colorectal cancer cell line in various oxygenation conditions

Abstract

We propose a new in vitro model to assess the impact of 90Y-microspheres derived low-dose beta radiation on colorectal cancer cell line under various oxygenation conditions that mimic the tumor environment. Cancer cells (HCT116) proliferation was assessed using Alamar Blue (AB) assay after 48, 72, and 96 h. FLUKA code assessed changes in cancer cell populations relative to the absorbed dose. In normoxia, mitochondrial activity measured by Alamar Blue after 48–72 h was significantly correlated with the number of microspheres (48 h: r = 0.87 and 72 h: r = 0.89, p < 0.05) and absorbed dose (48 h: r = 0.87 and 72 h: r = 0.7, p < 0.05). In hypoxia, the coefficients were r = 0.43 for both the number of spheres and absorbed dose and r = 0.45, r = 0.47, respectively. Impediment of cancer cell proliferation depended on the absorbed dose. Doses below 70 Gy could reduce colorectal cancer cell proliferation in vitro. Hypoxia induced a higher resistance to radiation than that observed under normoxic conditions. Hypoxia and radiation induced senescence in cultured cells. The new in vitro model is useful for the assessment of 90Y radioembolization effects at the micro-scale.

PET/CT with 18F-choline or 18F-FDG in Hepatocellular Carcinoma Submitted to 90Y-TARE: A Real-World Study

“…It is expected that 90 Y-TARE might also have delayed effects, although this assumption is mainly based on the limitations of traditional imaging techniques in the early assessment of response to 90 Y-TARE. In a recently published in vitro study, in fact, 90 Y-microspheres were found to reduce colorectal cancer cell proliferation as early as within 96 h of observation [ 26 ]. In this perspective, a time interval of 8 weeks after procedure might represent a reasonable gap to assess 90 Y-microsphere effects on HCC.…”

Radiomic signatures from T2W and DWI MRI are predictive of tumour hypoxia in colorectal liver metastases

“…Hypoxia-driven elevation of proangiogenic factors is a hallmark of colorectal cancer and its liver metastasis [ 18 ]. Specifically, in the treatment of colorectal liver metastases, the hypoxic status has also been shown to influence the resistance to antiangiogenic drugs [ 19 ] or their susceptibility regarding radiation therapy, thereby impacting the dosimetric planning for Y-90 radioembolisation [ 20 ]. Moreover, in the era of immune therapies, hypoxia has also been revealed to play a major role in the current understanding of the immune microenvironment of colorectal liver metastases [ 21 ].…”

“…Hypoxia-driven elevation of proangiogenic factors is a hallmark of colorectal cancer and its liver metastasis [18]. Specifically, in the treatment of colorectal liver metastases, the hypoxic status has also been shown to influence the resistance to antiangiogenic drugs [19] or their susceptibility regarding radiation therapy, thereby impacting the dosimetric planning for Y-90 radioembolisation [20].…”