A new indicator: The diagnostic value of CD8+T/B lymphocyte ratio in sepsis progression

Abstract

Objective To reveal the value of single lymphocyte subpopulation and their ratios in the progression of sepsis. Methods From January 2019 to March 2021, 39 sepsis patients, 16 septic shock patients, and 50 healthy volunteers were recruited in the Second Xiangya Hospital for this cross-sectional study. The absolute quantitation of CD4+T, CD8+T, B lymphocytes, and NK cells in peripheral blood were determined by flow cytometry. SPSS Software was used to analyze the results. Results On the whole, the numbers of lymphocytes in the sepsis group and in the septic shock group were lower than that in the healthy control group. Surprisingly, the percentage of CD8+T lymphocytes in the septic shock group was slightly higher than that in the sepsis group. The percentage of B lymphocytes in the sepsis group was higher than that in the healthy control group. The AUC of CD8+T/B was 0.724, with the sensitivity and specificity being 75.00% and 71.79%, respectively. Conclusion The immune expression pattern of patients with sepsis was not a simple decrease in the number of lymphocytes. The change in the ratios of lymphocyte subpopulation might be more meaningful along the development and progression of sepsis. The ratio of CD8+T/B could be used to diagnose the progression of sepsis and reduce the misdiagnosis rate to a certain extent.

Immune profile of patients‑a new approach in management of sepsis and septic shock?

“…The reported results regarding the changes of CD8 cells in septic shock are controversial. As in the present results, the CD8 cells decrease in septic shock is the finding of one study (39), but according to another study, the percentage of CD8+T lymphocytes in the septic shock group was slightly higher than that in the sepsis group (40).…”

Биологические Маркеры Сепсиса

“…Peng и др. [16] обнаружили, что соотношение CD8 + T-лимфоциты/B-лимфоциты может быть использовано для диагностики прогрессирования сепсиса -с чувствительностью и специфичностью 75.00 и 71.79% соответственно -и для оценки эффекта проводимой терапии.…”

Biological markers of sepsis

“…Li et al [15] reported that as sepsis progressed, CD3 + , CD4 + , and CD4 + /CD8 + counts in the blood of patients decreased, while elevated CD4 + and CD4 + /CD8 + counts correlated with patient recovery dynamics. Peng et al [16] found that the CD8 + T lymphocyte/B lymphocyte ratio can help diagnose the progress of sepsis (with sensitivity and specificity of 75.00% and 71.79%, respectively) as well as to assess the effect of the ongoing therapy.…”